Antidiabetic effect of Psychotria malayana Jack in induced type 1 diabetic rat

Fairuz, Fairuz; Dewi, Hasna; humaryanto, humaryanto

doi:10.30595/medisains.v18i1.6909

Cited by 2 publications

(3 citation statements)

References 18 publications

(22 reference statements)

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“…Percentage of DPPH scavenging activity = (A control − A sample )/A control × 100 (4) where A sample and A control represent the sample's or positive control's and negative control's absorbance, respectively [65].…”

Section: -Nbdg Uptake In 3t3-l1 Adipocyte Cellsmentioning

confidence: 99%

“…Psychotria malayana is a plant commonly available in Southeast Asian regions and is traditionally used to manage diabetes. The height of this plant is roughly about 1-4 m, and the Lombok people in Indonesia have utilized the water extract of this plant to prevent and treat some illnesses, including diabetes [3,4]. Despite its long history of traditional use as a therapeutic herb, the research on this plant's glucose uptake and antioxidant activity is limited.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary Phytochemical Screening, In Vitro Antidiabetic, Antioxidant Activities, and Toxicity of Leaf Extracts of Psychotria malayana Jack

Nipun

Khatib

Ahmed

et al. 2021

Plants

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Psychotria malayana Jack belongs to the Rubiacea and is widespread in Southeast Asian countries. It is traditionally used to treat diabetes. Despite its potential medicinal use, scientific proof of this pharmacological action and the toxic effect of this plant are still lacking. Hence, this study aimed to investigate the in vitro antidiabetic and antioxidant activities, toxicity, and preliminary phytochemical screening of P. malayana leaf extracts by gas chromatography-mass spectrometry (GC-MS) after derivatization. The antidiabetic activities of different extracts of this plant were investigated through alpha-glucosidase inhibitory (AGI) and 2-NBDG glucose uptake using 3T3-L1 cell line assays, while the antioxidant activity was evaluated using DPPH and FRAP assays. Its toxicological effect was investigated using the zebrafish embryo/larvae (Danio rerio) model. The mortality, hatchability, tail-detachment, yolk size, eye size, beat per minute (BPM), and body length were taken into account to observe the teratogenicity in all zebrafish embryos exposed to methanol extract. The LC50 was determined using probit analysis. The methanol extract showed the AGI activity (IC50 = 2.71 ± 0.11 μg/mL), insulin-sensitizing activity (at a concentration of 5 µg/mL), and potent antioxidant activities (IC50 = 10.85 μg/mL and 72.53 mg AAE/g for DPPH and FRAP activity, respectively). Similarly, the water extract exhibited AGI activity (IC50 = 6.75 μg/mL), insulin-sensitizing activity at the concentration of 10 μg/mL, and antioxidant activities (IC50 = 27.12 and 33.71 μg/mL for DPPH and FRAP activity, respectively). The methanol and water extracts exhibited the LC50 value higher than their therapeutic concentration, i.e., 37.50 and 252.45 µg/mL, respectively. These results indicate that both water and methanol extracts are safe and potentially an antidiabetic agent, but the former is preferable since its therapeutic index (LC50/therapeutic concentration) is much higher than for methanol extracts. Analysis using GC-MS on derivatized methanol and water extracts of P. malayana leaves detected partial information on some constituents including palmitic acid, 1,3,5-benzenetriol, 1-monopalmitin, beta-tocopherol, 24-epicampesterol, alpha-tocopherol, and stigmast-5-ene, that could be a potential target to further investigate the antidiabetic properties of the plant. Nevertheless, isolation and identification of the bioactive compounds are required to confirm their antidiabetic activity and toxicity.

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Section: -Nbdg Uptake In 3t3-l1 Adipocyte Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preliminary Phytochemical Screening, In Vitro Antidiabetic, Antioxidant Activities, and Toxicity of Leaf Extracts of Psychotria malayana Jack

Nipun

Khatib

Ahmed

et al. 2021

Plants

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“…18,19 In addition, central caseous necrosis is not always seen on small biopsies, and other granulomatous conditions are possible. 20 Low and high lymphocyte densities were found to have different proportions in LNTB patients. A total of 26 samples (86.7%) had low lymphocyte density, and 4 samples (13.3%) had high lymphocyte density.…”

Section: Discussionmentioning

confidence: 94%

Microscopic Features of Central Caseous Necrosis in Tuberculosis Lymphadenitis Patients: The Relationship with Lymphocyte Density

2023

bul.farmatera

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Tuberculosis lymphadenitis (TBLN) is the most common extra pulmonary TB (EPTB) case in the world with a range of 30-40% of all EPTB cases. When the body's immune system cannot kill the pathogen, the body will try to contain the pathogen (granuloma). The outer portion of the granuloma becomes calcified and the cells in the centre undergoes necrosis. The formation of central necrosis is associated with the emergence of cellular hypersensitivity with the features of local accumulation of macrophages and lymphocytes. The current study aimed to determine the relationship between the microscopic features of central caseous necrosis with lymphocyte density in patients with tuberculous lymphadenitis. Thirty histopathological slides of TBLN patients were observed under a microscope to see the features of central caseous necrosis and assess the lymphocyte density with the results assessed based on the cut-off point of the median of all lymphocyte mean values. Based on the microscopic examination, 25 samples of central caseous necrosis were found and the other 5 samples were not found to have caseous necrosis. Twenty-six patients had low lymphocyte density, and 4 patients had high lymphocyte density. The results of the Fisher Exact test obtained p = 0.009. There is a significant relationship between the features of central caseous necrosis and lymphocyte density in patients with TBLN.

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Antidiabetic effect of Psychotria malayana Jack in induced type 1 diabetic rat

Cited by 2 publications

References 18 publications

Preliminary Phytochemical Screening, In Vitro Antidiabetic, Antioxidant Activities, and Toxicity of Leaf Extracts of Psychotria malayana Jack

Preliminary Phytochemical Screening, In Vitro Antidiabetic, Antioxidant Activities, and Toxicity of Leaf Extracts of Psychotria malayana Jack

Microscopic Features of Central Caseous Necrosis in Tuberculosis Lymphadenitis Patients: The Relationship with Lymphocyte Density

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