Antidiabetic effects of flavonoids from Sophora flavescens EtOAc extract in type 2 diabetic KK-ay mice

Yang, Xinzhou; Yang, Jing; Xu, Chan; Mi, Huang; Zhou, Qi; Lv, Jingnan; Ma, Xiaochun; Ke, Chang‐Qiang; Yang, Yong; Gao, Shu; Zhao, Ping

doi:10.1016/j.jep.2015.05.043

Cited by 50 publications

(27 citation statements)

References 44 publications

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“…In this way, a moderate increase in the expression of GLUT4 is a good target for the treatment of IR in patients. In our present study, we established a L6-based GLUT4 translocation system to discover natural agents that may promote GLUT4 translocation to the cell membranes [46,47]. In an in vitro study, SMR exhibited strong stimulation of GLUT4 transportation onto plasma membranes.…”

Section: Discussionmentioning

confidence: 99%

Anti-diabetic activity of stigmasterol from soybean oil by targeting the GLUT4 glucose transporter

Wang

Yang

et al. 2017

Food & Nutrition Research

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104

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The present study investigated the anti-diabetic activity and potential mechanism of stigmasterol (SMR), which is a kind of phytosterols derived from the edible soybean oil in vitro and in vivo. SMR displayed a mild GLUT4 translocation activity by 1.44-fold in L6 cells. L6 cells were treated with different concentration of SMR, showing significant effects on the enhancing glucose uptake. SMR administrated orally to the KK-Ay mice significantly alleviated their insulin resistance and oral glucose tolerance with reducing fasting blood-glucose levels and blood lipid indexes such as triglyceride and cholesterol. Moreover, the GLUT4 expression in L6 cells, skeletal muscle and white adipose tissue had been also enhanced. In this paper we conclude that, stigmasterol seems to have potential beneficial effects on the treatment of type 2 diabetes mellitus with the probable mechanism of targeting GLUT4 glucose transporter included increasing GLUT4 translocation and expression.

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Section: Discussionmentioning

confidence: 99%

Anti-diabetic activity of stigmasterol from soybean oil by targeting the GLUT4 glucose transporter

Wang

Yang

et al. 2017

Food & Nutrition Research

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104

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“…A single dose in a particular animal species is administered for acute toxicity testing. During the 2-week observation period for acute oral toxicity testing (Yang et al, 2015), the measurements indicated no abnormality. The oral acute toxicity study on SB-EtOAc indicated that its single dose oral LD50 for KM mice was proposed to be more than 5400 mg/kg/day.…”

Section: Discussionmentioning

confidence: 98%

“…Based on this antidiabetic target, a cell-based GLUT4 translocation assay using stable L6 cells expressing pIRAP-mOrange cDNA was established to identify plants extracts, fractions, and their isolated compounds that may have potential antidiabetic effects, by evaluating their effects on the translocation of GLUT4 to the plasma membrane (Yang et al, 2015Wang et al, 2014). During the screening, SB-EtOAc exhibited strong stimulatory effects on GLUT4 translocation by 3.2-fold in L6 cells compared to the control group.…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic activity of perylenequinonoid-rich extract from Shiraia bambusicola in KK-Ay mice with spontaneous type 2 diabetes mellitus

Zhao

Xiong

et al. 2016

Journal of Ethnopharmacology

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“…The chemical review on Sophora tomentosa reveals that the presence of alkaloids, polysaccharides, isoprenylated flavonoids, isoflavonones, flavones, flavonols, their glycosides, and these compounds have been previously reported to have wide bioactivity including anti-diabetic, antioxidant, hepatoprotective and immune regulatory [5,6]. Recently, it was found that the ethyl acetate extract of roots of S. flavescens significantly controlled levels of insulin, through stimulation of GLUT4 which attenuated by AMP-activated protein kinase pathway and this extract was practically non-toxic for mice.…”

Section: The Hepatic Ameliorative Effect Of I Tricolor and S Tomentosamentioning

confidence: 99%

“…Compounds other than the bioactive flavonoids as alkaloids, coumarochromones, saponins, triterpene glycosides, phospholipids, polysaccharides, oligostilbenes, and fatty acids are reported in many Sophora species [5]. The roots of Sophora flavescens is one of the bitter and cold Chinese medicines commonly used to remove lung heat have been used to counteract diabetes mellitus (DM) and exerted good clinical effects for diabetic patients in some folk hospitals in Fujian province [6]. Thus, this study aims to evaluate the possible beneficial effect of Ipomoea tricolor and Sophora tomentosa crude extracts on serum lipid parameters in hypercholesterolemic (HC) and hyperglycemic (HG) rats as well as the histological examination of the liver and pancreas were carried out to confirm the biochemical findings and also to develop and utilize effective natural lipid and blood glucose regulators for more aggressive treatment of HC associated with HG.…”

Section: Introductionmentioning

confidence: 99%

Statistical Data Analysis Which Result From the Bio-Diagnosis and Bio-Treatment of Injured Rats With the Hyperlipidemia and Hyperglycemia Diseases

Abduallah¹,

Abd‐Alla²,

Ali³

et al. 2016

Asian J Pharm Clin Res

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Objective: This study in bioinformatics aims to investigate the potential effect of Ipomoea tricolor and Sophora tomentosa on liver function enzymes activity, serum lipid profile, oxidative stress biomarkers, and on blood glucose in high fat diet-induced hypercholesterolemia (HC) and STZ-induced hyperglycemia (HG) in rats.Methods: Blood glucose level, liver function enzymes, alanine aminotransferases and aspartate aminotransferases, alkaline phosphatase, and lactate dehydrogenase (LDH) were determined. Besides, lipid profile including total cholesterol (TC), triacylglycerol (TG), total lipid, and high-density lipoprotein-cholesterol was investigated. Moreover, oxidative stress biomarkers, lipid peroxide, and nitric oxide as well as non-enzymatic antioxidant, glutathione (GSH) were also examined in different therapeutic groups.Results: A significant increase in blood glucose level, liver function enzyme activities, LDH, lipid profile and oxidative stress markers, while significant decrease in LDH-C and GSH level in HC-HG induced rats compared to control one. A marked amelioration in all biochemical parameters under investigation on treatment of HC-HG rats with I. tricolor and S. tomentosa with different fluctuating percentages of improvement. Histopathological examination of liver and pancreas was also performed and declared HC-HG showed congestion in portal vessels and sinusoids with mild centrilobular hepatocyte degeneration, marked hepatocyte ballooning and hydropic degeneration, while HC-HG treated rats with I. tricolor and S. tomentosa showed normal lobular hepatic architecture with mild sinusoidal dilatation and congestion. On the other hand, a histological organization of pancreas of HC-HG rats showing disarrangement changes in pancreatic blood vessels and interlobular duct as well as disordered in acini. The treatment of HC-HG rats with I. tricolor and S. tomentosa showed enhancement in Langerhans cells and restore of most pancreatic tissue in comparison with standard drugs. Conclusion:The statistical results showed that each extract ameliorated high blood glucose level liver injury, HC and oxidative stress indicating relieving of oxidative damage associated with the complexity of HG and HC. These results demonstrated that these two plants extracts may be a candidate intelligent antioxidant, hypolipidemic, hypoglycemic, and hepatoprotective nutraceuticals which need further clinical investigation to be applied effectively to reduce perturbation in HC associated diabetes.

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Antidiabetic effects of flavonoids from Sophora flavescens EtOAc extract in type 2 diabetic KK-ay mice

Cited by 50 publications

References 44 publications

Anti-diabetic activity of stigmasterol from soybean oil by targeting the GLUT4 glucose transporter

Anti-diabetic activity of stigmasterol from soybean oil by targeting the GLUT4 glucose transporter

Antidiabetic activity of perylenequinonoid-rich extract from Shiraia bambusicola in KK-Ay mice with spontaneous type 2 diabetes mellitus

Statistical Data Analysis Which Result From the Bio-Diagnosis and Bio-Treatment of Injured Rats With the Hyperlipidemia and Hyperglycemia Diseases

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