Antidiabetic potential: <i>in vitro</i> inhibition effects of some natural phenolic compounds on α‐glycosidase and α‐amylase enzymes

Taslimi, Parham; Gülçın, İlhami

doi:10.1002/jbt.21956

Cited by 127 publications

(75 citation statements)

References 56 publications

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“…The results of IC 50 value for PLs indicate that HBA with 2.34 to 3.70 μM of IC 50 had lower PL inhibitory effects than that of a natural PL inhibitor, lipstatin (IC 50 , 0.14 μM), whereas HBA had efficacious PL inhibitory effects than those of gallic acid (active hydroxybenzoic acids, 59.4 μM), ferulic acid (123.9 μM) of active hydroxycinnamic acids, and kaempferol (2.5 μM) of active flavonoids . In addition, HBA with 9.08 μM of IC 50 had efficacious α‐glycosidase inhibitory effects than those of a standard α‐glycosidase inhibitor, acarbose (22.8 μM); however, the values were higher than those of some phenolic compounds …”

Section: Discussionmentioning

confidence: 96%

p‐Hydroxybenzyl alcohol inhibits four obesity‐related enzymes in vitro

Choi

Yeo

Kim

et al. 2018

J Biochem & Molecular Tox

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Recently, antiobesity studies using the method of inhibiting enzymatic activity of obesity‐related enzymes as targets have received considerable attention. The aims of the current study were to investigate whether p‐hydroxybenzyl alcohol (HBA), identified from Cudrania tricuspidata fruits with antiobesity effects, inhibits the activity of digestive and obesity‐related enzymes and acts as an inhibitor against four target enzymes in kinetic studies. In vitro enzyme assays showed HBA at the highest concentration significantly reduced the enzymatic activity of four targets: pancreatic lipase (IC50 = 2.34‐3.70 μM), α‐glycosidase (IC50 = 9.08 μM), phosphodiesterase IV (IC50 = 4.99 μM), and citrate synthase (IC50 = 2.07 μM) enzymes. Based on the results of kinetic assays, the types of inhibition were investigated. Our findings indicate that HBA could have antiobesity efficacy, and it deserves further study.

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Section: Discussionmentioning

confidence: 96%

p‐Hydroxybenzyl alcohol inhibits four obesity‐related enzymes in vitro

Choi

Yeo

Kim

et al. 2018

J Biochem & Molecular Tox

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“…Column flow rate was 20 mL/h, and 4 mL fractions were collected . Also, the protein flow in the column eluates was spectrophotometrically detected at 280 nm as explained formerly …”

Section: Methodsmentioning

confidence: 99%

Intermolecular amination of allylic and benzylic alcohols leads to effective inhibitions of acetylcholinesterase enzyme and carbonic anhydrase I and II isoenzymes

Atmaca

Yıldırım

Taslimi

et al. 2018

J Biochem & Molecular Tox

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In this study, we aimed to determine the inhibition effects of novel synthesized sulfamates (2a-g), sulfonamides (3b-f), carbonyl sulfonamides (3h and i), and carbonyl sulfamates (4h and 4i), which were tested against two human cytosolic carbonic anhydrase I and II isozymes (hCA I and II) and acetylcholinesterase (AChE) enzyme. For inhibition properties of allylic sulfamates, the half maximal inhibitory concentration (IC ) and inhibition constant (K ) were calculated for each novel compounds. The allylic sulfamates showed that K values are in the range of 187.33-510.31 pM for hCA I, 104.22-290.09 pM against hCA II, and 12.73-103.63 pM against AChE. The results demonstrated that all newly synthesized compounds had shown effective inhibition against hCA I and II isoenzymes and AChE enzyme.

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“…Nonetheless, these drugs can cause many side effects, which include flatulence, abdominal discomfit, and diarrhea; thus, discovering natural inhibitory alternatives with lower side effects and higher treatment effectiveness to replace these drugs is urgently needed (Pantidos et al, ). As reported in the previous researches, some natural phenolic compounds extracted from plant materials have great potential to be used as natural inhibitors on digestive enzymes (Taslimi & Gülçin, ; Taslimi, Akıncıoglu, & Gülçin, ; Taslimi, Caglayan, & Gülçin, ).…”

Section: Introductionmentioning

confidence: 94%

Optimization of arabinogalactan and taxifolin extraction process from Dahurian larch (Larix gmelinii ) and evaluation of the effects on activities of α-amylase, α-glycosidase, and pancreatic lipase in vitro

et al. 2018

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Double experimental designs, Plackett–Burman design coupled with Box–Behnken design, were used to optimize the simultaneous extraction process of arabinogalactan and taxifolin from Larix gmelinii by ultrasonic microwave synergistic extraction (UMSE) method. The optimal extraction conditions were determined with the extraction yields of 223.40 ± 7.82 mg/g for larch arabinogalactan and 125.02 ± 5.30 mg/g for taxifolin. Compared to conventional methods, UMSE method showed the merits of higher extraction efficiency, saving time, and energy. The molar ratio of galactose to arabinose is found to be 6:1 through the monosaccharide composition analysis of larch arabinogalactan. The obtained taxifolin structure was identified by mass spectrometry, Fourier transform infrared spectroscopy, and nuclear magnetic resonance. Interestingly, larch arabinogalactan and taxifolin showed completely opposite influences on α‐amylase, α‐glycosidase, and pancreatic lipase; it was inferred that larch arabinogalactan could act as an inhibitor while taxifolin as an accelerator. Practical applications In this study, response surface methodology of Plackett–Burman design and Box–Behnken design performed well to obtain the optimal conditions and satisfactory extraction yields for the extraction of arabinogalactan and taxifolin from Larix gmelinii by ultrasonic microwave synergistic extraction method, which could be a good paradigm for the efficient isolation of bioactive substances from plant matrix in the field of separation science. Meanwhile, the results from the evaluation of the effects of two target compounds on activities of α‐amylase, α‐glycosidase, and pancreatic lipase in vitro revealed that larch arabinogalactan and taxifolin enjoyed the completely opposite influences on the three digestive enzymes, namely larch arabinogalactan could act as an inhibitor while taxifolin an accelerator. These results will facilitate the application of larch arabinogalactan and taxifolin in the food and pharmaceutical industries.

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Antidiabetic potential: in vitro inhibition effects of some natural phenolic compounds on α‐glycosidase and α‐amylase enzymes

Cited by 127 publications

References 56 publications

p‐Hydroxybenzyl alcohol inhibits four obesity‐related enzymes in vitro

p‐Hydroxybenzyl alcohol inhibits four obesity‐related enzymes in vitro

Intermolecular amination of allylic and benzylic alcohols leads to effective inhibitions of acetylcholinesterase enzyme and carbonic anhydrase I and II isoenzymes

Optimization of arabinogalactan and taxifolin extraction process from Dahurian larch (Larix gmelinii ) and evaluation of the effects on activities of α-amylase, α-glycosidase, and pancreatic lipase in vitro

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