Antidiabetic potential of Bauhinia forficata Link leaves: a non-cytotoxic source of lipase and glycoside hydrolases inhibitors and molecules with antioxidant and antiglycation properties

Alves, Victor Hugo Mota; Zabisky, Luiz Fernando Ribeiro; Justino, Allisson Benatti; Martins, Mário Machado; Saraiva, André Lopes; Goulart, Luíz Ricardo; Espindola, Foued Salmen

doi:10.1016/j.biopha.2019.109798

Cited by 39 publications

(21 citation statements)

References 42 publications

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“…The glycation reaction is highly accelerated under hyperglycemia, implicating the pathogenesis of diabetics and aging. In addition, oxidative stress plays a role in forming AGEs, and thus which has been implicated in causing the progression of diabetes [ 34 ]. In view of this, the possible functions of anti-glycation of DBT with or without L. plantarum fermentation were determined here.…”

Section: Resultsmentioning

confidence: 99%

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Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Guo

Gao

et al. 2020

Chin Med

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Background Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix at a ratio of 5: 1, is prescribed for menopausal women. Flavonoids and its flavonoid glycosides are considered as the major active ingredients within the herbal decoction; however, their amount is not controllable during the preparation. Besides, the aglycons within DBT are believed to have better gut absorption and pharmacological efficacy. Methods The herbal extract of DBT was fermented with Lactobacillus plantarum. The amounts of flavonoid glucosides and its aglycones in the fermented product were analyzed by using UPLC-MS/MS. In addition, in vitro assays were employed to evaluate the efficacy of the fermented DBT in regulating the activities of α-glucosidase, α-amylase and lipase, as well as their antioxidant capacity (DPPH and T-AOC assays) and anti-glycation property (BSA-methylglyoxal, BSA-fructose, and arginine-methylglyoxal models). Results The fermentation of DBT with L. plantarum drove a completed conversion of calycosin-7-O-β-D-glucoside and ononin to calycosin and formononetin, respectively. The chemical transformation could be probably mediated by β-glycosidase within the fermented product. Several in vitro assays corresponding to anti-diabetic functions were compared between parental DBT against its fermented product, which included the activities against α-glucosidase, α-amylase and lipase, as well as anti-oxidation and anti-glycation. The fermented DBT showed increased activities in inhibiting α-glycosidase, suppressing DPPH radical-scavenging and anti-glycation, as compared to the original herbal product. Conclusion These results suggested that DBT being fermented with the probiotic L. plantarum could pave a new direction for fermentation of herbal extract, as to strengthen its pharmacological properties in providing health benefits.

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Section: Resultsmentioning

confidence: 99%

“…Pancreatic lipase: The pancreatic lipase activity was performed using PNPP as substrate [ 34 ]. PNPP was used as a substrate in a solution containing: 40 mg PNPP in isopropanol added to 50 mM Tris–HCl buffer (pH 8.0), 40 mg gum Arabic, 80 mg sodium deoxycholate, and Triton X-100.…”

Section: Methodsmentioning

confidence: 99%

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Guo

Gao

et al. 2020

Chin Med

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show abstract

“…The glycation reaction is highly accelerated under hyperglycemia, implicating the pathogenesis of diabetics and aging. In addition, oxidative stress plays a role in forming AGEs, and thus which has been implicated in causing the progression of diabetes [30]. In view of this, the possible functions of anti-glycation of DBT with or without L. plantarum fermentation were determined here.…”

Section: Methods Development and Optimizationmentioning

confidence: 99%

“…Pancreatic lipase: The pancreatic lipase activity was performed using PNPP as substrate [30]. PNPP was used as a substrate in a solution containing: 40 mg PNPP in isopropanol added to 50 mM Tris-HCl buffer (pH 8.0), 40 mg gum Arabic, 80 mg sodium deoxycholate, and Triton X-100.…”

Section: Uplc-ms/ms Analysismentioning

confidence: 99%

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Guo

Gao

et al. 2020

Preprint

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Background Danggui Buxue Tang (DBT), an ancient Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix at a ratio of 5: 1, is prescribed for menopausal women. Flavonoids and its flavonoid glycosides are considered as the major active ingredients within the herbal decoction; however, their amount is not controllable during the preparation. Besides, the aglycons within DBT are believed to have better gut absorption and pharmacological efficacy. Methods In order to enhance the therapeutic efficacy of DBT, the herbal extract was fermented together with Lactobacillus plantarum, a Gram-positive Lactobacillus commonly found in fermented food products. Results The fermentation of DBT with L. plantarum drove a completed conversion of calycosin-7-O-β-D-glucoside and ononin to calycosin and formononetin, respectively, as determined by UPLC-MS/MS analysis. The chemical transformation could be probably mediated by β-glycosidase within the fermented product. Several in vitro assays corresponding to anti-diabetic functions were compared between parental DBT against its fermented product, which included the activities against α-glucosidase, α-amylase and lipase, as well as anti-oxidation (e.g. DPPH radical-scavenging and total antioxidant capacities) and anti-glycation (e.g. BSA-methylglyoxal, BSA-fructose and arginine-methylglyoxal models). The fermented DBT showed increased activities in inhibiting α-glycosidase, suppressing DPPH radical-scavenging and anti-glycation, as compared to the original herbal product. Conclusion The results supported the possible application of L. plantarum or other Lactobacillus in fermenting herbal extract, as such to increase the yield and efficacy of the final products.

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“…Previous researches have shown that some types of tea exhibited pronounced inhibitory effects towards the activities of α-amylase, lipase and in ammation [8,9]. At present, many treatments involving the use of medicinal plants have been recommended, since most contain compounds with antidiabetic, anti-in ammatory, anti-pyretic and analgesic effects and with low indices of toxicity [5,10,11]. Plants are huge source of bioactive secondary metabolites such as glycosides, saponins, avonoids, steroids, tannins, alkaloids and terpenes, which act as strong antioxidant to protect body from oxidative damage [12].…”

Section: Introductionmentioning

confidence: 99%

Ephedra alata extracts exhibits anti-obesity, anti-hyperlipidaemic, anti-hyperglycemia, anti-antipyretic and analgesic effects through the inhibition of lipase, α-amylase and inflammation

Tiss¹,

Souiy²,

Boujbiha³

et al. 2020

Preprint

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In this present study, phytochemical screening, anti-obesity, anti- antipyretic and analgesic effect of Ephedra alata (EA) extracts were evaluated. Results of this study showed that the methanol EA extract (MEAE) had a stronger inhibition activity on key enzyme related to obesity as lipase (IC50 = 1.296 mg/ml) as compared to water and ethyl acetate extracts ( with IC50 = 1.639 and 1.897 respectively). Also results revealed that ethyl acetate extract depicted high activity toward key enzyme related to hyperglycemia as α-amylase (IC50 = 0.284 mg/mL) while water and methanol extracts showed good to moderate inhibitory activity (IC50 = 1,525 and 1,103 mg/ml respectively). In HFFD rats, the administration of MEAE at 200 mg/kg inhibited key enzyme related to fat digestion and absorption as lipase in the intestine, pancreas and serum by 53, 40 and 53% respectively,which leads to decrease in body weight by 20% and also modulation of lipid profile. In addition, the administration of MEAE to HFFD-rats exerts an inhibitory effect on α-amylase activity in the intestine, pancreas and serum by 43, 26 and 46% respectively and this leads to decrease in blood glucose level by 35%. Also, MEAE exert antipyretic and analgesic activities. This study demonstrates for the first time that MEAE are efficient in preventing obesity and hyperglycemia and liver toxicity. This data generated during study shows antipyretic and analgesic activities of MEAE.

show abstract

Antidiabetic potential of Bauhinia forficata Link leaves: a non-cytotoxic source of lipase and glycoside hydrolases inhibitors and molecules with antioxidant and antiglycation properties

Cited by 39 publications

References 42 publications

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Fermentation of Danggui Buxue Tang, an ancient Chinese herbal mixture, together with Lactobacillus plantarum enhances the anti-diabetic functions of herbal product

Ephedra alata extracts exhibits anti-obesity, anti-hyperlipidaemic, anti-hyperglycemia, anti-antipyretic and analgesic effects through the inhibition of lipase, α-amylase and inflammation

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