Antidiarrheal effect of sodium hydrosulfide in diabetic rats: In vitro and in vivo studies

Saghazadeh‐Dezfuli, M.; Fanaei, Hamed; Gharib-Naseri, Mohammad Kazem; Nasri, Sima; Mard, Seyyed Ali

doi:10.1111/nmo.13273

Cited by 5 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although different biological effects in some studies, H 2 S is viewed as one of three important gas biological molecules(together with NO and CO). Some studies showed that high concentration H 2 S induce IBD pathogenesis because of making toxicity for cells and genes by upregulating proin ammatory cytokines and damaging mucous layer and epithelial cells [16][17][18]. H 2 S is produced by two ways as biologically endogenous and exogenous ways.…”

Section: Discussionmentioning

confidence: 99%

Effect of Clostridium Butyricum to promote the intestinal barrier of DSS colitis by regulating endogenous hydrogen sulfide enzyme

Shen

Cheng

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Although Clostridium Butyricum ameliorates active ulcerative colitis (UC), the precise mechanism remains largely unclear. To investigate the mechanism of Clostridium Butyricum in protecting intestinal barrier of DSS colitis by regulating endogenous hydrogen sulfide enzyme. Methods Forty C57BL/6J male mice were randomly divided into control group, DSS colitis group, low-dose (107CFU/ml) Clostridium Butyricum group and high-dose (108CFU/ml) Clostridium Butyricum group. We use qRT-PCR and Western blot to assess the expression of CSE and CBS as endogenous hydrogen sulfide enzyme in colonic tissue of each group. However, the expression of ZO-1,Occludin, E-cadherin, Lypd8, MIF and DAI score, colon length, histological damage were separately used to evaluate the epithelial tighten junction, mucous barrier and epithelial inflammation in intestine. Result Both high-dose and low-dose live Clostridium Butyricum could significantly diminish the expression of CSE, CBS of colonic tissue in DSS colitis (P<0.05). The live Clostridium Butyricum dose-dependently inhibited the colonic length shorten, DAI scores and histological damage of DSS colitis with down-regulating the expression of MIF and increasing the expression of ZO-1, Occludin, E-cadherin and Lypd8 in DSS colitis (P<0.05). Conclusions The live Clostridium Butyricum ameliorates DSS colitis by suppressing the expression of CSE and CBS with the mechanism related to regulate the epithelial mucous barrier protein, protect the epithelial tighten junction and inhibit the inflammatory factor with the possible down-regulating the expression of hydrogen sulfide (H2S).

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Clostridium Butyricum to promote the intestinal barrier of DSS colitis by regulating endogenous hydrogen sulfide enzyme

Shen

Cheng

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Apoptosis of enteric glial cells may aggravate neuronal loss [1]. Another possible mechanism may be reduced synthesis of sodium hydrosulphide, which acts as an inhibitor of intestinal smooth muscles [45]. There are conflicting results regarding the role of bile acid malabsorption in diabetic diarrhoea, but the theory has recently gained new impetus [12,46].…”

Section: Discussionmentioning

confidence: 99%

Diabetic diarrhoea: A study on gastrointestinal motility, pH levels and autonomic function

2021

View full text Add to dashboard Cite

Background. Chronic diarrhoea is a common, but poorly investigated diabetes complication. Autonomic neuropathy is a leading pathophysiological theory founded on old, small studies. Studies of gastrointestinal motility and pH levels are lacking.Objectives. Using new diagnostic methods, we aimed to find out if diabetic diarrhoea was associated with alterations in gastrointestinal motility, pH levels and autonomic function.Methods. Fifty-seven patients (42 women, 46 with type 1 diabetes) were prospectively included. Symptoms were evaluated with the gastrointestinal symptom rating scale, defining ≥4 points as cases with diarrhoea. Patients scoring <4 were used as controls. We used the wireless motility capsule to measure gastrointestinal transit times, pH levels and contractility parameters. Autonomic function was assessed by measuring heart rate variability, baroreflex sensitivity and orthostatic hypotension.Results. Seventeen patients (30%) had diarrhoea. Compared with controls, cases had slower gastric emptying (21:46 vs. 4:14, h:min, p = 0.03) and faster colonic transit (18:37 vs. 54:25, p < 0.001). Cases had increased intraluminal pH in the antrum (2.4 vs. 1.2, p = 0.009), caecum (7.3 vs. 6.4, p = 0.008) and entire colon (7.1 vs. 6.7, p = 0.05). They also had a decreased pH difference across the pylorus (3.3 vs. 4.9, p = 0.004) and ileocaecal junction (0.6 vs 1.0, p = 0.009). The groups did not differ in autonomic function, but diastolic blood pressure drop correlated r s = −0.34 (p = 0.04) with colonic transit time. Conclusions.Patients with diabetic diarrhoea had altered gastrointestinal transit and intraluminal pH levels, but minimal changes in autonomic function. Our results suggest that tests of gastrointestinal function are clinically useful in diabetic diarrhoea.

show abstract

“…In the digestive system, CBS exists in the gastric mucosa, colonic epithelium, small intestine, jejunum, and ileum. [30][31][32][33] CBS is also significantly expressed in the spleen. 34 CBS has been suggested to play an important role in the female reproductive system since it is well expressed in the ovary and uterus but is relatively low in the prostate and testis.…”

Section: Enzymes That Synthesize H S 21 | the Distribution Of Cbs And...mentioning

confidence: 99%

“…CBS content in other tissues is relatively low. In the digestive system, CBS exists in the gastric mucosa, colonic epithelium, small intestine, jejunum, and ileum 30–33 . CBS is also significantly expressed in the spleen 34 .…”

Section: Enzymes That Synthesize H2smentioning

confidence: 99%

Recent advances in the role of endogenous hydrogen sulphide in cancer cells

Chen

Qin

et al. 2023

Cell Proliferation

View full text Add to dashboard Cite

Hydrogen sulphide (H2S) is a gaseous neurotransmitter that can be self‐synthesized by living organisms. With the deepening of research, the pathophysiological mechanisms of endogenous H2S in cancer have been increasingly elucidated: (1) promote angiogenesis, (2) stimulate cell bioenergetics, (3) promote migration and proliferation thereby invasion, (4) inhibit apoptosis and (5) activate abnormal cell cycle. However, the increasing H2S levels via exogenous sources show the opposite trend. This phenomenon can be explained by the bell‐shaped pharmacological model of H2S, that is, the production of endogenous (low concentration) H2S promotes tumour growth while the exogenous (high concentration) H2S inhibits tumour growth. Here, we review the impact of endogenous H2S synthesis and metabolism on tumour progression, summarize the mechanism of action of H2S in tumour growth, and discuss the possibility of H2S as a potential target for tumour treatment.

show abstract

Antidiarrheal effect of sodium hydrosulfide in diabetic rats: In vitro and in vivo studies

Cited by 5 publications

References 21 publications

Effect of Clostridium Butyricum to promote the intestinal barrier of DSS colitis by regulating endogenous hydrogen sulfide enzyme

Effect of Clostridium Butyricum to promote the intestinal barrier of DSS colitis by regulating endogenous hydrogen sulfide enzyme

Diabetic diarrhoea: A study on gastrointestinal motility, pH levels and autonomic function

Recent advances in the role of endogenous hydrogen sulphide in cancer cells

Contact Info

Product

Resources

About