Antidiarrhoael and antimicrobial effects of ethanol root bark extract from <i>Salacia lehmbachii</i>

Akuodor, Godwin Christian; Essiet, Grace Akanimo; Ekenjoku, John A.; Udoh, Francis V.; Ogiji, Emeka; Ibiam, Gideon Ama; Nnorom, Friday Onyemazuwa

doi:10.34172/jhp.2021.20

Cited by 3 publications

(3 citation statements)

References 24 publications

(23 reference statements)

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“…The procedure outlined by Akuodor et al [13] was used for the test. Thirty Wistar rats were used for this test and were divided in five groups of six rats each.…”

Section: Castor Oil-induced Diarrheal Modelmentioning

confidence: 99%

Evaluation of Medicago sativa ethanol leaf extract for antidiarrheal activity in Wistar rats

Anele Donatus Onyebuchi,

Akuodor Godwin Christian,

Okoroama Casmir Emeka

et al. 2024

GSC Biol. Pharm. Sci.

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Aim: Medicago sativa leaf is traditionally used for the treatment of gastrointestinal disorders in Nigeria. This research investigates the in vivo antidiarrheal activity of M. sativa (Alfalfa) leaf extract in Wistar rats. Method: The study employed three models: castor oil-induced diarrhea, gastrointestinal motility (charcoal meal), and castor oil-induced intestinal fluid accumulation. The phytochemical analysis as well acute toxicity tests were carried out in the leaf extract. Results: The results demonstrate a dose-dependent and significant antidiarrheal effect of M. sativa leaf extract. In the castor oil-induced diarrhea model, the extract reduced fecal frequency, delayed onset, and lowered severity, with the highest effect at 600 mg/kg compared to a positive control (Loperamide). Gastrointestinal motility was inhibited by the extract in a dose-dependent manner, achieving maximum effect at 600 mg/kg, comparable to atropine sulfate. The castor oil-induced intestinal fluid accumulation model revealed a significant decrease in fluid volume at 600 mg/kg, exhibiting a potent inhibitory effect. The oral LD50 values obtained were greater than 5000 mg/kg in rats Conclusion: This study provides compelling evidence of Medicago sativa leaf extract's potential as an antidiarrheal agent in Wistar rats. Further investigations could explore its mechanism of action and safety profile, contributing valuable insights to the development of novel antidiarrheal therapies.

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“…The procedure outlined by Akuodor et al [13] was used for the test. Thirty Wistar rats were used for this test and were divided in five groups of six rats each.…”

Section: Castor Oil-induced Diarrheal Modelmentioning

confidence: 99%

Evaluation of Medicago sativa ethanol leaf extract for antidiarrheal activity in Wistar rats

Anele Donatus Onyebuchi,

Akuodor Godwin Christian,

Okoroama Casmir Emeka

et al. 2024

GSC Biol. Pharm. Sci.

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“…The minimum inhibitory concentration (MIC) of the extract was assayed through micro dilution method. 25 Ethanol root bark extract of H. annuus which inhibits growth of one or more microorganisms was examined for MIC. Serial dilutions of the extract were prepared to the concentrations of 6.25, 12.5, 25, 50, 100 and 125 μg/ ml.…”

Section: Determination Of Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…The MIC of ethanol root bark extract of H. annuus against the organisms were obtained as follows: S. aureus (20), K. pneumonia (22), C. albicans (35), Aspergillus niger (40) and E. coli (25).…”

Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

Evaluation of the antiulcer and antimicrobial activities of methanol leaf extract of Helianthus annuus

Ajegi

Ajegi²,

Ajaegbu³

et al. 2023

Int J Basic Clin Pharmacol

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Background: Medicinal plants are widely used in treating and preventing specific diseases and are known to play an important role in health care. Helianthus annuus is one of such plants whose therapeutic applications no doubt have numerous folkloric background. This study aimed at assessing Helianthus annuus methanol leaf extract for antiulcer and antimicrobial potentials Methods: The antiulcer activity was evaluated using aspirin, ethanol and histamine methods in Wistar rats, while the antimicrobial activity was carried out on selected microorganisms. The acute toxicity test and phytochemical screening of the extract were also conducted. Results: The methanol leaf extract exhibited significant (p<0.05 and p<0.01) antiulcer effect in both model. The ulcer protection effect of the extract is comparable to omeprazole. The extract also significantly inhibited the growth of test organisms. The acute toxicity test produced no lethality in rats, whereas the phytochemical analysis revealed the presence of alkaloids, saponins, tannins, flavonoids, terpenoids and steroids. Conclusions: The findings confirm the ethnomedicinal use of H. annuus leaf as a valuable natural agent for the treatment of ulcer and microbial infections.

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Anti-nociceptive, anti-inflammatory and antipyretic activities of the ethanol root bark extract of Salacia lehmbachii in rats and mice

Akuodor¹,

Ohadoma

Ofor

et al. 2021

Int J Basic Clin Pharmacol

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Background: The decoction of the roots of Salacia lehmbachi is used in traditional medicine for the treatment different diseases such as malaria pains diabetes and microbial infections.Methods: Phytochemical screening and oral acute toxicity tests were carried out on the ethanol root extract of the plant. Anti-nocicetive activity using acetic acid induced writhing and tail immersion method in mice, anti-inflammatory activity using carrageenan induced paw oedema in rats and xylene induced ear oedema test in mice and antipyretic activity using Brewer’s yeast and D-amphetamine induced pyrexia in rats were determined at 50 mg/kg, 100 mg/kg and 200 mg/kg doses of the root extract.Results: The ethanol root extract contain alkaloids, saponins, tannins, flavonoids, terpenoids, steroids and cardiac glycosides. The oral acute toxicity tests was found to be greater than 5000 mg/kg. The root extract and the standard drug (Aspirin) significantly (p<0.05 and p<0.01) decreased the number of writhes caused by acetic acid. The extract and morphine significantly (p<0.05 and p<0.01) prolonged reaction time in tail immersion model. The extract produced significant (p<0.05 and p<0.01) dose dependent inhibition of oedema which was comparable to aspirin in carrageenan induced paw oedema model. The root extract also demonstrated significant (p<0.05 and p<0.01) effect in xylene induced mouse ear oedema test compared to dexamethasone. The extract significantly decreased high temperature in both Brewer’s yeast and d-amphetamine induced pyrexia.Conclusions: Findings show that S. lehmbachii may provide a good source of plant compounds with analgesic, anti-inflammatory and antipyretic activities.

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Antidiarrhoael and antimicrobial effects of ethanol root bark extract from Salacia lehmbachii

Cited by 3 publications

References 24 publications

Evaluation of Medicago sativa ethanol leaf extract for antidiarrheal activity in Wistar rats

Evaluation of Medicago sativa ethanol leaf extract for antidiarrheal activity in Wistar rats

Evaluation of the antiulcer and antimicrobial activities of methanol leaf extract of Helianthus annuus

Anti-nociceptive, anti-inflammatory and antipyretic activities of the ethanol root bark extract of Salacia lehmbachii in rats and mice

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