Antidiarrhoeal Effects of Hydromethanolic Leaves Extract of Ipomea asarifolia in Albino Rat Model

Abstract

Aim: To evaluate the antidiarrhoea effect of hydromethanolic leave extract of I. asarifolia (HLEIA) on castor oil-induced diarrhea.
 Place and Duration of Study: Department of Biochemistry, Faculty of Life sciences, Kebbi State University of Science and Technology, Aliero, Kebbi state, Nigeria. P.M.B.1144. Kebbi State. Nigeria, between February 2015 and September 2016.
 Methodology: In a continuous effort to search for bioactive agents from medicinal plants, the antidiarrhoea activity of I. asarifolia was investigated. The effect of hydromethanolic leave extract of I. asarifolia (HLEIA) on castor oil-induced diarrhoea, gastrointestinal transit and intestinal fluid accumulation (enteropooling) were assessed in albino rats. Qualitative phytochemical analysis was carried out using standard procedures while acute oral toxicity studies was determined using the staircase method.
 Results: The phytochemical analysis showed the presence of alkaloid, terpenoid, tannin, saponin, phenols. The LD50 was estimated to be greater than 3000 mg/kg since there was no mortality recorded after 14 days of acute oral toxicity studies. Sub-chronic administration of graded doses (150 – 600 mg/kg) of HLEIA significantly (p<0.05) reduced diarrhoea episodes, decreased gastro intestinal movement and inhibited intestinal fluid accumulation compared to the control. The antidiarrhoea effect of treated group (600 mg/kg ) was comparable to that of the standard drug Loperamide.
 Conclusion: The findings of the present study scientifically validate the use of I. asarifolia in the treatment of diarrhoea.

Ethnopharmacological Survey, Phytochemical Screening and Antimicrobial Activities of Medicinal Plants Used in the Treatment of Diarrhea in Southeastern Gabon

“…Indeed, it has been shown in the literature that phenols, flavonoids and alkaloids can reduce or eliminate infectious microorganisms and have phospholipid activity [24]. Furthermore, it has been demonstrated by numerous works that the anti-diarrheal properties of medicinal plants are conferred by the presence of alkaloids, saponosides, terpenoids, flavonoids and sterols [25][26][27]. These phytomolecules are able to absorb electrolytes and water at the intestinal lumen [25], thus decreasing gastrointestinal motility [27].…”

“…Furthermore, it has been demonstrated by numerous works that the anti-diarrheal properties of medicinal plants are conferred by the presence of alkaloids, saponosides, terpenoids, flavonoids and sterols [25][26][27]. These phytomolecules are able to absorb electrolytes and water at the intestinal lumen [25], thus decreasing gastrointestinal motility [27]. The presence of these metabolites in plants used for the treatment of diarrhea by the populations in the Haut-Ogooué and Ogooué-Lolo provinces in Gabon suggests their anti-diarrheal properties and proven efficacy.…”

Evaluation of Anti-Diarrheal Activities of the 80% Methanol Extract and Solvent Fractions of Maesa lanceolata Forssk (Myrsinaceae) Leaves in Mice

“…Furthermore, ricinoleic acid causes localized intestinal mucosa irritation and inflammation, which causes the release of PG and an increase in the net secretion of water and electrolytes into the small intestine. 50,51 It is indicated that prostaglandin biosynthesis inhibitors prolong the time of onset of diarrhea caused by castor oil. 51,52 Another study revealed that the leaf extract of Maesa lanceolata has in vitro Cyclooxygenase 1 and 2 inhibitory activity.…”

Wound Healing Potentials of Methanolic Leaf Extract of <i>Piliostigma thonningii</i> Schum

“…), cytokines epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), granulocyte-macrophage colony-stimulating factor (GMCSF) and extracellular matrix (Martin and Nunan, 2015).Tissue regeneration is mostly sustained by multiple mechanisms involving constituents of the plants that induce healing (Nayak and Pereira, 2006;Akinmoladun et al, 2007).These constituents include various chemical families like alkaloids, essential oils, flavonoids, tannins, terpenoids, saponins, and phenolic compounds (Edeoga et al, 2005). Ukwuani-Kwaja et al, (2019), similarly detected the presence of saponins, tannins, alkaloids, anthraquinones, steroids and glycosides in the methanolic extract of P. thonningii leaves. The presence of these phytoconstituents which are largely phenolics as shown in this study,have been demonstrated to promote wound healing via various cellular-protein binding biochemical interactions (Dharmananda, 2003;Sanjay et al, 2018;Selvi et al, 2011;Scalbert 1991;Maikai and Kobo,2009).…”

“…Dry pods are roasted and ground into powder and mixed with tobacco powder and ashes of the red-leaf Amaranthus to make cooking soda (Coates Palgrave, 2002). Though several conventional drugs are known to promote wound healing, these drugs are however expensive with limited availability especially in rural communities (Ukwuani-kwaja et al, 2019). This study was designed to investigate the wound healing potential of P. thonningii leaves using wistar rats.…”