Antidyslipidemia and Antioxidant Activity of Andrographolide Compound From Sambiloto (Andrographis Paniculata) Herb

Abstract

Objective: The aim of this study was to observe the antidyslipidemic and antioxidant activity of andrographolide (AND) compound from Sambiloto (Andrographis paniculata) herb. The antidyslipidemic activity could be described by lowering TC (total cholesterol), TG (triglyceride), and LDL (low-density lipoprotein) level in the blood and by increasing HDL (high-density lipoprotein) level in the blood. The antioxidant activity could be described by inhibiting the ox-LDL (oxidized low-density lipoprotein) formation.Methods: AND was separated from the sambiloto ethanolic extract. Atherosclerosis in rats was induced by rich fat food (5% yolk, 10% lard), 1% calcium and 20.000 IU vitamin D3 for 90 d. The rats were treated with AND compound on the 61st day for 30 d. On the 90th day, the blood of rats had been collected, and its blood vessel was also extracted. Antidyslipidemic activity was measured by estimating the level of TC, TG, LDL and HDL in the blood. Antioxidant activity was measured by calculating the ox-LDL concentration in blood and histopathology parameter.Results: 18 mg/kg BW AND could reduce the level TC, TG, and LDL in blood, and could increase HDL blood level significantly (p<0.05). This compound can also significantly inhibit LDL oxidation (p<0.05), and the histopathology result showed that this compound could protect the vessel from adhesive lipid plaques.Conclusion: AND compound from sambiloto herb showed the antidyslipidemic and antioxidant effect in atherogenic rats.

Effects of Andrographis paniculata Extract on Toll-Like Receptor-4 (TLR4) Expression and Aorta Calcification in Rats Exposed to Cigarette Smoke

“…-NF--ERK2 on platelets. [6,17,18] In this study, the Andrographis paniculata extract was shown to decrease the expression of TLR4 with a decreasing effect starting to appear in treatment group 1, namely at a dose of 18 mg/kg BW, and there was a tendency for effect to increase with increasing dose. It was found that the dose of Andrographis Vol.8; Issue: 10; October 2021 paniculata extract in treatment group 3 (40 mg/kg BW) gave the highest TLR4 reduction effect.…”

“…[6,7] Andrographolide showed the antiatherosclerosis effect of Andrographis paniculata also as an anti-inflammation and had a potent hypolipidemic effect by lowering total cholesterol levels such as triglycerides, HDL, and LDL. [6,7] Andrographis paniculata is also known to be able to reduce inflammation and is said to have antioxidant properties by preventing the production of free radicals. [4,8] The purpose of this study was to prove whether the administration of Andrographis paniculata extract could reduce the expression of Toll Like Receptor-4 in rats exposed to cigarette smoke and could reduce the incidence of aortic calcification in rats exposed to cigarette smoke.…”

Pharmacological Potential of Andrographis paniculata (Burm. f.) Nees in Preventing Atherosclerosis: A Review

“…In both in vivo and human clinical trials, AP can enhance lipid profiles by lowering total cholesterol, triglycerides, LDL, and boosting HDL blood levels. 16,20,21,23,29,30,34 . This ability is caused by AP inhibition of the HMG-CoA reductase enzyme.…”

Employment of Atr-Ftir and HPLC-Uv Method for Detection and Quantification of Andrographolide

“…This compound becomes the point of interest since A. paniculata herb is widely used as a traditional medicine in several Asian countries such as India, Indonesia, China, Malaysia, and Thailand [1,2]. A large number of studies were conducted to isolate, identify, and quantify this substance [3][4][5] as well as assess its pharmacological activities [6][7][8], and its safety [9,10]. Andrographolide is reported to exhibit various biological activities, including antidiabetic [11], anticancer, anti-inflammatory [6], antivirus, and hepatoprotective activities.…”