Antiestrogenic and antigenotoxic activity of bee pollen from Cystus incanus and Salix alba as evaluated by the yeast estrogen screen and the micronucleus assay in human lymphocytes

Pinto, Barbara; Caciagli, Francesca; Riccio, Elisabetta; Reali, Daniela; Šarić, Ana; Balog, Tihomir; Likić, Saša; Scarpato, Roberto

doi:10.1016/j.ejmech.2010.06.001

Cited by 39 publications

(32 citation statements)

References 51 publications

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“…9 Kaempferol and quercetin can be used as markers for determining the quality of bee pollen, suggesting their potential role in prevention of free radical-implicated diseases such as cancer, cardiovascular diseases and also diabetes, among others. 10,11 Bee pollen has been shown to possess antimicrobial, 12,13 antifungal, 12,13 antioxidant, 11,13,14 anti-radiation, 15 hepatoprotective, 16,17 antiestrogenic, 18,19 antigenotoxic/antimutagenic, 13,18 anticancerogenic, 18 antiinflammatory, 13 and various anti-histopathologic capacities. 20 Pollen has been described as "nature's perfect food" for centuries and is a highly concentrated food source.…”

Section: Introductionmentioning

confidence: 99%

Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

Toman¹,

Hájková²,

Hluchý³

2015

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Context: Bee pollen has been related to therapeutic properties including antibiotic, antineoplasic, antidiarrhoeic and antioxidant activities. However, little is known about the effect of pollen feeding on the intestinal structures and functions. Aims: The objective of this study was to evaluate the small intestine morphology in rats fed with three different pollen levels. Materials and Methods: Forty rats were randomly separated into 4 groups of 10 rats each. Control group (C) was fed with a basic diet, group L received a diet supplemented with 0.2% (w/w), group M with 0.5% (w/w) and group H with 0.75% (w/w) bee pollen for 90 days. The relative volume of intestinal mucosa structures, length of villi and Lieberkühn crypts formation were evaluated. Results: Quantitative morphometry and histology methods revealed significant increase in the relative volume of epithelium (P<0.0001) and decrease in the connective tissue volume (P<0.0001) of jejunum in groups M and H as compared to the control. The intestinal villi length significantly (P<0.0001) increased in all experimental groups. On the other hand, the Lieberkühn crypts depth significantly (P<0.0001) increased only in groups L and M but decreased (P<0.0001) in the highest pollen-exposed group H. Conclusion: The most significant results were recorded in the medium pollen exposed group. We conclude that the bee pollen concentration of 0.5% is the most effective in the increase of relative volume of the intestinal epithelium and villi length. The results demonstrated that bee pollen affects the small intestine development in a concentration-dependent manner and could be beneficial for intestinal function.Key words: Bee pollen, Histology, morphometry, Rat, Small intestine. PICTORIAL ABSTRACT SUMMARY• Bee pollen enhances the develop ment of the small intestine in rats from 4 weeks to 4 months of age • Intestinal epithelium volume, villi length and crypts depth increase.• Bee pollen acts in a concentration-dependent manner.Abbreviations used: w/w: Percent Concentration Weight/Weight.

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Section: Introductionmentioning

confidence: 99%

Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

Toman¹,

Hájková²,

Hluchý³

2015

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“…When analyzing and studying the nutritional and therapeutic properties of bee pollen, modern science has made it possible to specify its valuable antimicrobial [2], antifungal [3], antioxidant [4], anti-radiation [5], hepatoprotective [6], chemopreventive [7], anticancer [8] and antiinflammatory activities [9].…”

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confidence: 99%

Chemical composition and antioxidant activity of <i>Apis mellifera</i> bee pollen from northwest Algeria

Rebiai¹,

Lanez²

2015

J. Fundam and Appl Sci.

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The aim of this study was to assess the nutritional composition and antioxidant activity of bee pollen produced in the North western region of Algeria. The content of total phenolic compounds, flavonoids, and antioxidant activity using the molybdate ion reduction method were determined. The mean contents of phenolic compounds and total flavonoids were 30.46 ± 8.22 mg of GAE.g -1 pollen and 8.92 ± 5.5 mg of RE.g -1 pollen, respectively. High antioxidant activities were found for the molybdate ion reduction method, with values that ranged from 71.95 to 101.5 μg of GAE.g -1 pollen. The bee pollen of Boufarik showed high antioxidant activity probably due to the high content of phenolic compounds present in pollen.

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“…Some of them are achilleine, apigenin, luteolin, azulene, camphor, coumarin, inulin, menthol, quercetin, rutin, succinic, salicylic and caffeic acids 3 . On the other hand, luteolin, quercetin, chrysin, and kaempferol have antiestrogenic effect 8 .…”

Section: Introductionmentioning

confidence: 99%

Radiolabeling of methanol extracts of yarrow (Achillea millefolium l) in rats

Cekic

Kılçar²,

Müftüler

et al. 2012

Acta Cir. Bras.

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PURPOSE: Current study is focused on extraction with methanol, purification, labeling with 131 I using iodogen method of the yarrow plant and investigating in vivo biological activity using biodistribution and imaging studies on healthy animal models. The aim of the study is to contribute plant extracts to discover new drugs in the diagnosis and treatment of several diseases. METHODS: Nine female and nine male healthy Wistar albino rats, which were approximately 100-150 g in weight, were used for biodistribution studies. For imaging studies four healthy male Balb-C mice were used. Quality control studies were done utilizing thin layer radio chromatography (TLRC) and high performance liquid chromatography (HPLC) methods. For biodistribution studies, 131 I radiolabeled Peak 7 ( 131 I-Peak 7) was sterilized and injected into the tail veil of rats and imaging studies were obtained using Kodak FX PRO in vivo Imaging System. RESULTS: The radiolabeling yield of each purified the bioactive extracts of the yarrow plant, seven peaks was between 79 and 92%. The highest radiolabeling yield was calculated for 131 I radiolabeled seventh peak ( 131 I-Peak 7) (92.78±5.04, n=5). For this reason the biodistribution and imaging studies were done for 131 I-Peak 7. That's why; these studies with Peak 7 were carried out. CONCLUSION: Peak 7 was radiolabeled with 131 I in high yield for using imaging and therapeutic studies in nuclear medical applications. Key words: Achillea. Luteolin. Iodine Isotopes. Chromatography, Liquid. Diagnostic Imaging. Rats. RESUMO OBJETIVO:O atual estudo tem por objetivo a extração com metanol, purificação, marcação com I 131 usando o método direto de marcação da planta Achillea, para investigar in vivo a atividade biológica usando biodistribuição e estudos de imagem em modelos animais saudáveis. O objetivo do estudo é contribuir com extratos de plantas para descobrir novas drogas para o diagnóstico e tratamento de várias doenças. MÉTODOS: Nove fêmeas e nove machos ratos Wistar albino saudáveis, com aproximadamente 100 a 150g de peso foram usados para estudos de biodistribuição. Para estudos de imagem, quatro camundongos Balb-C machos e saudáveis foram usados. Estudos de controle de qualidade foram realizados usando métodos de cromatografia de camada fina e cromatografia líquida de alta performance. Para estudos de biodistribuição, pico 5 radiografado com I 131 (I 131 -Peak 7) foi esterilizado e injetado na veia da cauda dos ratos e estudos de imagem foram obtidos usando Sistema de Imagem Kodak FX PRO in vivo. RESULTADOS: O retorno radiomarcado de cada extrato bioativo purificado da planta Achillea sete picos estavam entre 79 e 92%. O retorno com maior marcação foi calculado para I 131 sétimo pico (I 131 -Peak 7) (92,78±5,04, n=5). Por esta razão os estudos de biodistribuição e de imagem foram feitos para I 131 -Peak 7. CONCLUSÃO: Peak 7 foi radiomarcado com I 131 em alto retorno para uso em estudos terapêuticos e de imagens nas aplicações médicas nucleares. Descritores: Achillea. Luteolina. Isótop...

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Antiestrogenic and antigenotoxic activity of bee pollen from Cystus incanus and Salix alba as evaluated by the yeast estrogen screen and the micronucleus assay in human lymphocytes

Cited by 39 publications

References 51 publications

Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

Chemical composition and antioxidant activity of <i>Apis mellifera</i> bee pollen from northwest Algeria

Radiolabeling of methanol extracts of yarrow (Achillea millefolium l) in rats

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