Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium via Modulation of Oxidative Stress Signaling in a Mouse Model

Abstract

Antrodia cinnamomea, a folk medicinal mushroom, has numerous biological effects. In this study, we aim to assess whether the antifatigue effects of A. cinnamomea mycelia (AC) and its underlying mechanisms are related to oxidative stress signaling using behavioral mouse models and biochemical indices detection. Mice were orally treated with AC at doses of 0.1, 0.3, and 0.9 g/kg for three weeks. AC had no effect on the spontaneous activities of mice indicating its safety on central nervous system. Furthermore, results obtained from weight-loaded forced swimming test, rotary rod test, and exhausted running test confirmed that AC significantly enhanced exercise tolerance of mice. Biochemical indices levels showed that these effects were closely correlated with inhibiting the depletion of glycogen and adenosine triphosphate stores, regulating oxidative stress-related parameters (superoxide dismutase, glutathione peroxidase, reactive oxygen species, and malondialdehyde) in serum, skeletal muscle, and liver of mice. Moreover, the effects of AC may be related with its regulation on the activations of AMP-activated protein kinase, protein kinase B, and mammalian target of rapamycin in liver and skeletal muscle of mice. Altogether, our data suggest that the antifatigue properties of AC may be one such modulation mechanism via oxidative stress-related signaling in mice.

Flavonoids in Cancer Metastasis

“…The effectiveness of chemotherapy in colorectal cancer patients evaluated as combined with the herbal agent MB-6 composed of fermented soybean extract, green tea extract, Antrodia camphorata mycelia, spirulina, grape seed extract, and curcumin extract. Major constituents of MB-6 [ 122 ] are known to contain various flavonoids [ 123 , 124 , 125 , 126 , 127 ]. A proof-of-concept clinical study conducted on 72 metastatic colorectal cancer patients randomized to receive leucovorin, 5-fluoroacil, and oxaliplatin in combination with MB-6 or placebo for a period of 16 weeks.…”

Griseococcin (1) from Bovistella radicata (Mont.) Pat and antifungal activity

“…Previously, many authors reported the various biological activity of fermentation broth from puffball like anticancer activity [26,27], antioxidant activity [28], antifatigue effect [29], etc. In the present study, the antifungal activity of B. radicata was another important biological function.…”

Triterpenoids Extracted From Antrodia cinnamomea Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice via Suppression Inflammatory Response

“…Antrodia cinnamomea mycelia were obtained by submerged fermentation as previously described (Liu et al, 2017a ). Triterpenoids were extracted in 80% ethanol twice at 80°C for 100 min.…”