According to the survey materials of the waters of Jiaozhou in April and August 1981, the author determined the variation range of temperature, location, and the variation process of isothermal water mass in the waters of Jiaozhou Bay. The results showed that in April, high-temperature water masses were formed in the coastal waters of Jiaozhou Bay from the northeast to the northwest, and the water temperature reached 12.82–13.70°C, where the length of the interval of seawater temperature change was 0.88°C. In August, the high-temperature water masses were formed in the coastal waters of Jiaozhou Bay from the northwest to the north with the high temperature within 27.32–27.37°C, where the length of the interval was 0.05°C. In April and August, the high-temperature water masses were formed in the northern coastal waters where the depth was 1.00–5.00 m. The heat of high-temperature water masses was sourced from solar radiation energy. In August and in the coastal waters near the estuary of Haibo River, high-temperature water masses were formed, with a high water temperature of 28.00–30.90°C. The high temperature of the Haibo River provided heat to the waters of Jiaozhou Bay. In April, a circular low-temperature water mass was formed in the central waters of the bay, and the water temperature reached a low temperature of 7.52–8.51°C, which indicated that the central waters of the bay still maintained the uniform low temperature in winter and formed a low-temperature water mass. Therefore, the innovation of this paper is to put forward the spatio-temporal variation of the isothermal water mass in Jiaozhou Bay, established the block diagram of temperature changes in the waters of Jiaozhou Bay in April and August, and further elaborated the characteristics of isothermal water masses. (1) At the same time change, both the high-temperature water mass and the low-temperature water mass have stability. (2) In different time changes, whether it is a high-temperature water mass with a relatively higher water temperature or a high-temperature water mass with a very high water temperature, the water mass is stable.