Antifertility activity of dihydroelatericin a in the female mouse

Shohat, B.; Beemer, A. M.; Gitter, S.; Lavie, David

doi:10.1007/bf01946171

Cited by 27 publications

(10 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histological studies of the ovaries show that this drug inhibits ovulation for 4 months in experimental animals at 27.5"C. The action mechanism seems to be related with the endocrine biochemistry of the reproductive system (Shohat et al, 1972).…”

Section: Anovulatory Activitymentioning

confidence: 98%

“…The semisynthetic dihydro-cucurbitacin D presents antifertility activity when orally administered to female mice over a minimum of 2 weeks. Histological studies of the ovaries show that this drug inhibits ovulation for 4 months in experimental animals at 27.5"C. The action mechanism seems to be related with the endocrine biochemistry of the reproductive system (Shohat et al, 1972).…”

Section: Anovulatory Activitymentioning

confidence: 99%

See 1 more Smart Citation

Cucurbitacins and their pharmacological effects

Miró

1995

Phytotherapy Research

181

View full text Add to dashboard Cite

show abstract

Section: Anovulatory Activitymentioning

confidence: 98%

Section: Anovulatory Activitymentioning

confidence: 99%

Cucurbitacins and their pharmacological effects

Miró

1995

Phytotherapy Research

181

View full text Add to dashboard Cite

show abstract

“…Withaferin A and withanolide D have, among others, the following effects: Withaferin A inhibits adjuvant arthritis in rats and the graft versus host rection in chicken [9]. Withaferin A and withanolide D inhibit the xenogenic graft versus host reaction in rats [10] and the formation of human E and EAC rosettes [10]. The steroidal alkaloid solasodine has been shown to cause thymolysis in rats [11] and to have antiphlogistic properties in experimental arthritis in rats and in experimental bums in rabbit ears [12].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulating Properties of 5,20α(R)-Dihydroxy-6α,7α-epoxy-1-oxo-(5α)-witha-2,24-dienolide and Solasodine

Bähr¹,

Hänsel²

1982

Planta Med

View full text Add to dashboard Cite

show abstract

“…2 Since the early discovery of WFA during the 1960s, the major interest has been on its anti-tumor cytotoxic activities. 3,4 However, the non-cytotoxic anti-inflammatory 5 and immunomodulatory mechanisms 6 of WFA have thus far remained rather poorly characterized. These latter disease-altering activities are highly pertinent to the practice of ayurveda, a traditional form of Indian medicine, which has borne out many effective formulations from W. somnifera, especially for the treatment of chronic human diseases such as arthritis and female bleeding disorders.…”

mentioning

confidence: 99%

Development of withaferin A analogs as probes of angiogenesis

Yokota¹,

Bargagna‐Mohan²,

Ravindranath³

et al. 2006

Bioorganic & Medicinal Chemistry Letters

View full text Add to dashboard Cite

The natural product withaferin A (WFA) is a potent angiogenesis inhibitor and it targets the ubiquitin-proteasome pathway in vascular endothelial cells. We generated a biotinylated affinity analog WFA-LC 2 B for use as a probe to study angiogenesis. WFA-LC 2 B inhibits angiogenic sprouting in vitro and it causes levels of ubiquitinated proteins to increase in tumor necrosis factor-α-treated human umbilical vein endothelial cells, confirming the retention of WFA's biological activity. We show that WFA-LC 2 B forms protein adducts in endothelial cells which are competed by free WFA in vivo. This WFA-LC 2 B analog will be useful to isolate the biological target of WFA. KeywordsBiotinylated analog; Natural product; Binding protein; Ubiquitin; Angiogenesis inhibitor Withaferin A (WFA), an important prototype of the withanolide class of natural products ( Fig. 1), is a highly oxygenated steroidal lactone that is found in the medicinal plant Withania somnifera and its related solanaceas species. 1 The withanolides are known to exert very potent and diverse cytotoxic, anti-stress, cardioactive, central nervous system, and immunomodulatory activities. 2 Since the early discovery of WFA during the 1960s, the major interest has been on its anti-tumor cytotoxic activities. 3,4 However, the non-cytotoxic anti-inflammatory 5 and immunomodulatory mechanisms 6 of WFA have thus far remained rather poorly characterized. These latter disease-altering activities are highly pertinent to the practice of ayurveda, a traditional form of Indian medicine, which has borne out many effective formulations from W. somnifera, especially for the treatment of chronic human diseases such as arthritis and female bleeding disorders. 2 Angiogenesis, which is the growth of new blood vessels from preexisting vasculature, is a pathogenic manifestation in cancers, 7 and it is also widely recognized to be critically involved in the pathogenesis of arthritis, endometriosis, age-related macular degeneration, diabetic retinopathy, etc. 7 Since these non-malignant inflammatory diseases could also benefit from anti-angiogenic therapeutics, 8 that such extracts could possess heretofore unrecognized inhibitors of angiogenesis. In fact, we demonstrated that W. somnifera extracts containing non-cytotoxic levels of withanolides, and also WFA, the derived active principle of these extracts, exert potent anti-angiogenic activity in vivo at very low doses. 10 Furthermore, at low nanomolar concentrations, we showed that WFA directly targets endothelial cell proliferation and exerts cytostatic cell cycle G 1 arrest in human umbilical vein endothelial cells (HUVECs). Interestingly, noncytotoxic sub-to-low micromolar concentrations of WFA also inhibit in vitro vessel formation 10,11 in the three-dimensional endothelial cell sprouting assay (3D-ECSA). At such doses, WFA potently inhibits TNF-α-induced NF-κB-DNA-binding activity, a mechanism which is associated with stabilization of phosphorylated IκB-α in the cytoplasm. Our findings suggest that WFA does not in...

show abstract

Antifertility activity of dihydroelatericin a in the female mouse

Cited by 27 publications

References 3 publications

Cucurbitacins and their pharmacological effects

Cucurbitacins and their pharmacological effects

Immunomodulating Properties of 5,20α(R)-Dihydroxy-6α,7α-epoxy-1-oxo-(5α)-witha-2,24-dienolide and Solasodine

Development of withaferin A analogs as probes of angiogenesis

Contact Info

Product

Resources

About