Antifouling Activities of Antagonistic Marine Bacterium Pseudomonas putida Associated with an Octopus

Viju, Nadarajan; Satheesh, Sathianeson; Punitha, S.

doi:10.1007/s40011-015-0691-x

Cited by 14 publications

(10 citation statements)

References 43 publications

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“…was incorporated into synthetic wood polish (Varnish) and tested for antifouling activity in the coastal waters. Many previous studies have also reported the antifouling performance of coatings developed with the crude bacterial extracts (Satheesh et al 2012;Viju et al 2014Viju et al , 2017. However, these studies including the present study were conducted for a short period of field trials.…”

Section: Discussionmentioning

confidence: 78%

“…A small quantity of TLC purified EPS was placed on the face of a highly polished KBr salt plate and another KBr plate was positioned on the top to spread the compound as a thin layer. The frequencies were measured as wave numbers over the range of 400 to 4,000 cm -1 (Viju et al 2017).…”

Section: Analysis Of the Functional Groups Present In The Eps Using Fmentioning

confidence: 99%

“…The bacteria associated with marine algae and other organisms are reported to produce a higher percentage of bioactive compounds comparing to free floating bacteria (Zheng et al 2005). Hence, a consequent attention has been paid on marine bacteria associated with the surface of invertebrates for the isolation of novel antifouling compounds (Satheesh et al 2012;Viju et al 2017). The production of compounds such as Giffinisterone B, Lobocompacto and Dicitrinin A etc.…”

Section: Introductionmentioning

confidence: 99%

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Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Viju¹,

Ezhilraj²,

Shankar³

et al. 2018

Nova Biotechnologica Et Chimica

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Bacteria associated with surfaces have been frequently cited as a potential source for the isolation of bioactive metabolites. In this study, bacteria associated with marine gastropod, Babylonia sp. were isolated and screened for antibacterial activity against biofilm-forming bacteria. The antibiofilm and antifouling effect of the selected surface- associated bacterial strains were examined under in vitro and in vivo conditions. Results showed that the extracellular polymeric substances (EPS) of the bacterial strain CML associated with gastropod species considerably reduced the adhesion of biofilm-forming bacteria on glass coupons. Besides, the antifouling coat prepared by incorporating of this EPS into polyurethane varnish prevented the settlement of biofoulers on test substratum submerged in marine waters. The functional groups present in the EPS were analyzed using FT-IR. The bacterium responsible for the production of the bioactive EPS was identified as Bacillus subtilis subsp. by 16S rRNA gene sequencing. More detailed characterization of the identified bioactive EPS could lead to the isolation of a novel natural antifouling product.

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Section: Discussionmentioning

confidence: 78%

Section: Analysis Of the Functional Groups Present In The Eps Using Fmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Viju¹,

Ezhilraj²,

Shankar³

et al. 2018

Nova Biotechnologica Et Chimica

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“…The organic layer was concentrated using the vacuum rota evaporator. The resulting crude extract was recuperated in DMSO and bioassayed against test strains [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Spectroscopic and chromatographic identification of bioprospecting bioactive compounds from cow feces: Antimicrobial and antioxidant activities evaluation of gut bacterium Pseudomonas aeruginosa KD155

Gupta

Rana

2021

Biotechnology Reports

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Highlights Isolation and screening of P. aeruginosa from cow dung having antagonistic potential. Molecular characterization of the isolate and thermodynamic stability study. Antimicrobial activity of extracellular crude extract of P. aeruginosa against microorganisms of medical importance. Antioxidant activity of extracellular crude extracts using DPPH scavenging activity. Isolation and characterisation of bioactive compound using HP-TLC, FTIR and GC-MS.

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“…The PCR reaction steps such as denaturation (at 95°C for 30 s), annealing (at 52°C for 30 s) and extension (at 60°C for 4 min) were carried out until the production of an adequate number of nucleotides (bps). The obtained sequence was analysed using the Basic Local Alignment Search Tool (BLAST), and their similarity was compared with the other bacterial sequences available in the National Center for Biotechnology Information (NCBI) (Viju et al, 2017). The sequence data of all the three symbiotic bacteria (B. firmus, B. cereus and B. subtilis) were deposited in GenBank, and their accession numbers have been assigned by NCBI.…”

Section: Identification Of Bioactive Bacterial Symbiontsmentioning

confidence: 99%

Antibiofilm activity of symbiotic Bacillus species associated with marine gastropods

Viju¹,

Punitha²,

Satheesh

2020

Ann Microbiol

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Purpose: Generally, symbiotic marine bacteria are renowned for the synthesis of compounds with bioactive properties, and this has been documented in many previous studies. Therefore, the present study was aimed to isolate novel bacterial symbionts of gastropods that have the ability to synthesize bioactive compounds. These bioactive compounds could be used effectively as antibiofilm agents in order to overcome the problems associated with biofilm. Methods: The bacteria associated with the surface of marine gastropods were isolated and characterized. Following this, the bacterial metabolites were extracted and their antibiofilm effect was evaluated on biofilm-forming bacteria on artificial substrates. Moreover, the biofilm-forming bacterium Alteromonas sp. was treated with the extracts of symbiotic bacteria in order to evaluate the influence of extracts over the synthesis of extracellular polymeric substance (EPS). Besides, the biologically active chemical constituents of the extracts were separated using thin-layer chromatography and subjected to gas chromatography and mass spectrometry (GC-MS) analysis for characterization. Results: Three bacterial strains belonging to the species Bacillus firmus, Bacillus cereus and Bacillus subtilis were identified from the bacterial community associated with the gastropods. The antibiofilm assays revealed that the extract of three symbiotic bacteria significantly (p < 0.05) reduced the biofilm formation by the marine bacterium Alteromonas sp. on artificial materials. Also, the EPS synthesis by Alteromonas sp. was significantly inhibited due to symbiotic bacterial extract treatment. The chemical composition of the bioactive fraction isolated from the symbiotic bacteria extract revealed that most of the detected compounds were belonging to aromatic acid, fatty acid and carboxylic acid. Conclusion: The results of this study clearly revealed that the bacteria belonging to the above listed Bacillus species can be considered as a promising source of natural antibiofilm agents.

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Antifouling Activities of Antagonistic Marine Bacterium Pseudomonas putida Associated with an Octopus

Cited by 14 publications

References 43 publications

Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Spectroscopic and chromatographic identification of bioprospecting bioactive compounds from cow feces: Antimicrobial and antioxidant activities evaluation of gut bacterium Pseudomonas aeruginosa KD155

Antibiofilm activity of symbiotic Bacillus species associated with marine gastropods

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