Antifouling Activity of Prodigiosin from Estuarine Isolate of Serratia marcescens CMST 07

Priya, Kumutha; Satheesh, Sathianeson; Ashokkumar, Balasubramaniem; Varalakshmi, Perumal; Selvakumar, G.; Sivakumar, N.

doi:10.1007/978-81-322-1087-0_2

Cited by 22 publications

(16 citation statements)

References 37 publications

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“…The MIC values for different Bacillus sp. strains were in the range between 5 and 7 mg L -1 (MIC values for B. amyloliquefaciens FZB42 was 6.1 ± 1.2 mg L -1 , B. licheniformis ATTC9445A was 6.9 ± 2.2 mg L -1 , B. mycoides was 1.2 mg L -1 , B. subtilis NCIB3610 was 5.2 ± 1.4 mg L -1 , B. subtilis ATCC6051 was 5.9 mg L -1 and B. subtilis PS-216 was 5.9 mg L -1 ) and were lower as previously reported ( Stankovic et al, 2012 ; Priya et al, 2013 ). Approximately the same MIC values for different Bacillus species may suggest that prodigiosin has the same mode of action on all tested Bacillus sp.…”

Section: Resultssupporting

confidence: 41%

Prodigiosin Induces Autolysins in Actively Grown Bacillus subtilis Cells

et al. 2016

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Prodigiosin produced by marine bacterium Vibrio ruber DSM 14379 exhibits a potent antimicrobial activity against a broad range of Gram positive and Gram negative bacteria. The mechanism of prodigiosin antimicrobial action, however, is not known. In this work, the effect of prodigiosin on Bacillus subtilis growth, cell membrane leakage, and induction of autolysins was studied. Treating B. subtilis with prodigiosin resulted in rapid decline of optical density and increased cell membrane leakage measured by β-galactosidase activity. Cell lysis was initiated immediately after treatment with prodigiosin in the middle exponential phase and was completed within 2 h. Lytic activity of prodigiosin in mutant strains with impaired autolysin genes lytABCD decreased for 80% compared to the wild type strain, while in lytABCDEF mutant strain prodigiosin had no bacteriolytic but only bacteriostatic effect. Fast prodigiosin lytic activity on individual B. subtilis cells was confirmed by a modified comet assay. The results indicate that prodigiosin autolysin induction in B. subtilis is growth phase dependent.

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Section: Resultssupporting

confidence: 41%

Prodigiosin Induces Autolysins in Actively Grown Bacillus subtilis Cells

et al. 2016

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“…Prodigiosin is known to have antibiotic activity; however, its activity against Pseudomonas species is discussed controversially ( Samrot et al, 2011 ; Gulani et al, 2012 ; Priya et al, 2013 ; Sumathi et al, 2014 ). Reasons may include different experimental set-ups and strain specific characteristics.…”

Section: Resultsmentioning

confidence: 99%

“…Reasons may include different experimental set-ups and strain specific characteristics. We therefore analyzed susceptibility of the here used P. putida strain KT2440 in a simple disk diffusion assay in direct comparison to Bacillus subtilis and human pathogen Staphylococcus aureus that were previously reported as susceptible ( Samrot et al, 2011 ; Gulani et al, 2012 ; Priya et al, 2013 ), and in comparison to E. coli , an alternative heterologous host for prodigiosin production. The zones of inhibition observed for B. subtilis and S. aureus confirmed previous reports ( Figure 4A ).…”

Section: Resultsmentioning

confidence: 99%

Efficient recombinant production of prodigiosin in Pseudomonas putida

et al. 2015

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Serratia marcescens and several other bacteria produce the red-colored pigment prodigiosin which possesses bioactivities as an antimicrobial, anticancer, and immunosuppressive agent. Therefore, there is a great interest to produce this natural compound. Efforts aiming at its biotechnological production have so far largely focused on the original producer and opportunistic human pathogen S. marcescens. Here, we demonstrate efficient prodigiosin production in the heterologous host Pseudomonas putida. Random chromosomal integration of the 21 kb prodigiosin biosynthesis gene cluster of S. marcescens in P. putida KT2440 was employed to construct constitutive prodigiosin production strains. Standard cultivation parameters were optimized such that titers of 94 mg/L culture were obtained upon growth of P. putida at 20°C using rich medium under high aeration conditions. Subsequently, a novel, fast and effective protocol for prodigiosin extraction and purification was established enabling the straightforward isolation of prodigiosin from P. putida growth medium. In summary, we describe here a highly efficient method for the heterologous biosynthetic production of prodigiosin which may serve as a basis to produce large amounts of this bioactive natural compound and may provide a platform for further in-depth studies of prodiginine biosynthesis.

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“…However, no antimicrobial activity was found against E. coli. In another study, Priya et al (2013), found that B. subtilis (8 mm), P. aeruginosa (6 mm), Alteromonas sp. (17 mm) and Gallionella sp.…”

Section: Discussionmentioning

confidence: 96%

Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

Lapenda

Silva

Vicalvi

et al. 2014

World J Microbiol Biotechnol

132

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Prodigiosin is an alkaloid and natural red pigment produced by Serratia marcescens. Prodigiosin has antimicrobial, antimalarial and antitumor properties and induces apoptosis in T and B lymphocytes. These properties have piqued the interest of researchers in the fields of medicine, pharmaceutics and different industries. The aim of the present study was to evaluate the antimicrobial activity of prodigiosin against pathogenic micro-organisms. The red pigments produced by S. marcescens exhibited absorption at 534 nm, Rf of 0.59 and molecular weight of 323 m/z. Antimicrobial activity was tested against oxacillin-resistant Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Streptococcus pyogenes, Acinetobacter sp. and oxacillin-resistant S. aureus. The standard antibiotics employed were ampicillin, chloramphenicol, gentamicin and oxacillin. The disc-diffusion tests demonstrated significant inhibition zones for S. aureus (35 ± 0.6), E. faecalis (22 ± 1.0) and S. pyogenes (14 ± 0.6). However, prodigiosin showed resistance to E. coli, P. aeruginosa and acinetobacter, where no significant formation of inhibitory halos were observed. We determined the inhibitory minimum concentrations and bactericidal for 20 strains of oxacillin-resistant S. aureus (ORSA). The pattern was the antibiotic oxacillin. The minimum inhibitory concentrations observed ranged from 1, 2 and 4.0 μg/mL, respectively, while the minimum bactericidal concentrations ranged from 2, 4, 8 and 16 μg/mL. The S. marcescens prodigiosin produced by showed bactericidal and bacteriostatic effect showing promising antimicrobial activity and suggesting future studies regarding its applicability in antibiotics therapies directed ORSA.

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Antifouling Activity of Prodigiosin from Estuarine Isolate of Serratia marcescens CMST 07

Cited by 22 publications

References 37 publications

Prodigiosin Induces Autolysins in Actively Grown Bacillus subtilis Cells

Prodigiosin Induces Autolysins in Actively Grown Bacillus subtilis Cells

Efficient recombinant production of prodigiosin in Pseudomonas putida

Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398

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