Antifouling biocides: Impairment of bivalve immune system by chlorothalonil

Guerreiro, Amanda da Silveira; Rola, Regina Coimbra; Rovani, Monique Tomazele; Costa, Simone Rutz da; Sandrini, Juliana Zomer

doi:10.1016/j.aquatox.2017.06.012

Cited by 47 publications

(10 citation statements)

References 38 publications

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“…Chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile) is one of the most commonly used non-absorbable broad-spectrum fungicides, which is widely used in fruits, vegetables, grains and other crops[1]. Due to the widespread use of chlorothalonil, it is frequently reported that chlorothalonil remains in the atmosphere, water, soil and other environmental media[2-3].…”

Section: Instructionsmentioning

confidence: 99%

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

Gong

Yang

et al. 2020

Preprint

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A whole-cell biosensor developed with yeast Saccharomyces cerevisia to detect the toxicity of chlorothalonil has been developed. This biosensor relied on the inhibition effect for metabolism by toxicants to provide detection and the degree of toxicity to yeast cells. In order to further improve the toxic sensitivity of yeast cells biosensor, the effect of the action time, the initial pH value of the medium and the temperature on inhibiting cell growth were investigated. 10Response surface regression analysis was conducted to obtain optimal culture conditions. Th effects of treated yeast morphology, ROS, DNA, caspase 3 activity were analyzed. This optimized yeast as a biosensor was used to detect chlorothalonil and heavy metals. The results are as follows: at optimal culture conditions, EC50 values of chlorothalonil to yeast biosensor determined at incubation time 4 h increased from 0.25 µg·mL -1 in the control to 0.006 µg·mL -1 , which increased 15 by 41.67 times. Compared with the control yeast cells, the morphology of optimized yeast cells were more transparent, with significantly increased intracellular vesicle rate and cell membrane permeability, intracelluar ROS increased siginificantly, DNA bands extracted was ladder, and caspase 3 activity was stimulated. The yeast biosensor had a high sensitivity to heavy metals. After analysis, many treated cells were apoptosis which was the main reason for the increasing 20 sensitivity to detect harmful substances. It was found that the method provides a new idea for the detection of harmful substances in the environment.

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Section: Instructionsmentioning

confidence: 99%

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

Gong

Yang

et al. 2020

Preprint

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“…In 1987 was created an orthotopic model of CRC in mice with injection of tumor cells in the ceacum, which enabled the study of local tumor invasion as well as metastatic dissemination—it was a more patient‐like animal tumor model . The success of this model was very high, turning it into a valuable asset in the study of the CRC, and amply used, with even some adaptations as injection of tumor cells in the rectum, and even exploring microvascular patterns of the colon concerning the differences between the mesenteric and antimesenteric side…”

Section: What Type Of Crc Models Exist?mentioning

confidence: 99%

The role of mouse models in colorectal cancer research—The need and the importance of the orthotopic models

Oliveira

Abrantes

Tralhão

et al. 2020

Anim Models and Exp Med

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Colorectal cancer is a worldwide health burden, with high incidence and mortality, especially in the advanced stages of the disease. Preclinical models are very important and valuable to discover and validate early and specific biomarkers as well as new therapeutic targets. In order to accomplish that, the animal models must replicate the clinical evolution of the disease in all of its phases. In this article, we review the existent mouse models, with their strengths and weaknesses in the replication of human cancer disease progression, with major focus on orthotopic models. K E Y W O R D Scolorectal cancer, mouse model, orthotopic model

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“…Studies were done on several cell types; from embryonic cells and spermatozoa to adult cells such as haemocytes, gill cells and digestive gland cells. Among many marine species, most of the studies were done on the blue mussel (Mytilus edulis) [81-88], Mediterranean mussel (Mytilus galloprovincialis) [89][90][91][92][93][94][95][96][97][98][99][100] and bay mussel (Mytilus trossulus) [101,102] although there are studies done on several other mussels such as the Asian green mussel (Perna viridis) [103][104][105], New Zealand greenlipped mussel (Perna canaliculus) [106], brown mussel (Perna perna) [107,108] as well as the hydrothermal vent mussel (Bathymodiolus azoricus) [109]. The comet assay was also done on several other species of oysters, scallops, shells and clams, namely the Pacific oyster (Crassostrea gigas) [110][111][112][113][114], eastern oyster (Crassostrea virginica) [115,116], marine rock oyster (Saccostrea cucullata) [117,118], Farrer's scallop (Chlamys farreri) [119,120], grooved carpet shell (Ruditapes decussatus) [99,121,122], peppery furrow shell (Scrobicularia plana) [123][124][125], pullet carpet shell (Venerupis pullastra) [94], bean clam (Donax faba)…”

Section: Bivalvesmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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Antifouling biocides: Impairment of bivalve immune system by chlorothalonil

Cited by 47 publications

References 38 publications

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

The role of mouse models in colorectal cancer research—The need and the importance of the orthotopic models

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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