“Antifreeze” Glycoproteins from Polar Fish

Harding, Margaret M.; Anderberg, Pia I.; Haymet, A. D. J.

doi:10.1002/chin.200338271

Cited by 34 publications

(54 citation statements)

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“…One of the antifreezing strategies is to accumulate antifreezing agents in subfreezing conditions to minimize intracellular ice formation. Natural antifreezing agents include glycerol, glucose trehalose, sorbitol, polyols in various insects (Clark and Worland, 2008;Doucet et al, 2009) and proteinaceous antifreeze molecules in polar fishes (Harding et al, 2003), insects (Clark and Worland, 2008;Doucet et al, 2009), and diatoms (Gwak et al, 2009). In laboratory environments, similar challenges were faced in the development of technologies to control biological activities in live samples by cryopreservation.…”

Section: Introductionmentioning

confidence: 98%

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Tsang

Chow

2010

Birth Defects Research Pt C

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Rodent transgenesis and human-assisted reproductive programs involve multistep handling of preimplantation embryos. The efficacy of production and quality of results from conventionally scheduled programs are limited by temporal constraints other than the quality and quantities of embryos per se. The emergence of vitrification, a water ice-free cryopreservation technique, as a reliable way to arrest further growth of preimplantation embryos, provides an option to eliminate the time constraint. In this article, current and potential applications of cryopreservation to facilitate laboratory animal experiments, colony management, and human-assisted reproductive programs are reviewed. Carrier devices developed for vitrification in the last two decades are compared with an emphasis on their physical properties that infer cooling rate of samples and sterility assurance. Biological impacts of improved cryopreservation on preimplantation embryos are also discussed.

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Section: Introductionmentioning

confidence: 98%

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Tsang

Chow

2010

Birth Defects Research Pt C

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“…T he formation and growth of ice are highly unfavourable in most biological systems, with the exception of certain extremophiles including numerous Antarctic and Arctic fish species 1,2 . Ice formation and growth not only lead to mechanical damage on a cellular level, but also osmotic shock as the concentration of extracellular solutes rises as the liquid water volume fraction decreases 3 .…”

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confidence: 99%

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

et al. 2014

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The cryopreservation of cells, tissue and organs is fundamental to modern biotechnology, transplantation medicine and chemical biology. The current state-of-the-art method of cryopreservation is the addition of large amounts of organic solvents such as glycerol or dimethyl sulfoxide, to promote vitrification and prevent ice formation. Here we employ a synthetic, biomimetic, polymer, which is capable of slowing the growth of ice crystals in a manner similar to antifreeze (glyco)proteins to enhance the cryopreservation of sheep and human red blood cells. We find that only 0.1 wt% of the polymer is required to attain significant cell recovery post freezing, compared with over 20 wt% required for solvent-based strategies. These results demonstrate that synthetic antifreeze (glyco)protein mimics could have a crucial role in modern regenerative medicine to improve the storage and distribution of biological material for transplantation.

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“…2 Evidence shows the loss of organized ice structure at the interface as being fairly gradual, occurring over approximately 10 Å. 3 Very recently Ebbinghaus et al 7 have also questioned the previous assumption of hydrogen bonding as a dominant mechanism. By using terahertz spectroscopy they showed that antifreeze activity could be induced because the AFGP perturbs the aqueous solvent over long distances.…”

Section: Proposed Mechanism Of Antifreeze Activitymentioning

confidence: 97%

Antifreeze glycoprotein agents: structural requirements for activity

2011

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Antifreeze glycoproteins (AFGPs) are considered to be the most efficient means to reduce ice damage to cell tissues since they are able to inhibit growth and crystallization of ice. The key element of antifreeze proteins is to act in a non-colligative manner which allows them to function at concentrations 300-500 times lowers than other dissolved solutes. During the past decade, AFGPs have demonstrated tremendous potential for many pharmaceutical and food applications. Presently, the only route to obtain AFGPs involves the time consuming and expensive process of isolation and purification from deep-sea polar fishes. Unfortunately, it is not amenable to mass production and commercial applications. The lack of understanding of the mechanism through which the AFGPs inhibit ice growth has also hampered the realization of industrial and biotechnological applications. Here we report the structural motifs that are essential for antifreeze activity of AFGPs, and propose a unified mechanism based on both recent studies of short alanine peptides and structure activity relationship of synthesized AFGPs.

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“Antifreeze” Glycoproteins from Polar Fish

Abstract: For Abstract see ChemInform Abstract in Full Text.

Cited by 34 publications

References 1 publication

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

Antifreeze glycoprotein agents: structural requirements for activity

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