Antifungal Actions of Glycinin Basic Peptide against Aspergillus niger through the Collaborative Damage to Cell Membrane and Mitochondria

Feng, L.H.; Li, Yingqiu; Wang, Zhaosheng; Qi, Lianqing; Mo, Haizhen

doi:10.1007/s11483-018-9561-4

Cited by 13 publications

(7 citation statements)

References 46 publications

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“…In normal cells, Rhodamine 123 can enter the mitochondrial matrix through a normal MMP. When membrane integrity is damaged, Rhodamine 123 is released from the mitochondrial matrix and emits a strong yellow-green fluorescent signal . In this study, the change in the MMP of P.…”

Section: Results and Analysismentioning

confidence: 99%

“…Then, the plates were placed in a plastic box containing 0, 50, 100, and 200 mg/L of ClO 2 . After the plates were cultured for 6 d at 25 °C, approximately 5 × 7 mm mycelial segments were taken from the edge of the culture and fixed with 2.5% (v/v) glutaraldehyde at 4 °C for 12 h. Next, the fixed sample was washed three times with distilled water, dehydrated in an ethanol series (30,50,70, and 95%) for 20 min in each concentration, and placed in absolute ethanol for 45 min. Subsequently, the samples were dried in a vacuum freeze dryer and platinized with an ion sputter coater.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Chlorine Dioxide Controls Green Mold Caused by Penicillium digitatum in Citrus Fruits and the Mechanism Involved

Liu

Jiao

et al. 2020

J. Agric. Food Chem.

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Green mold caused by Penicillium digitatum is the main postharvest disease in citrus fruits. The goal of this study is to evaluate the antifungal activity of chlorine dioxide (ClO 2 ) against P. digitatum both in vivo and in vitro and to elucidate the underlying mechanism using flow cytometry and scanning electron microscopy. The results showed that 200−1800 mg/L of ClO 2 significantly inhibited the incidence of green mold on kumquats, mandarins, Peru's oranges, and grapefruits caused by P. digitatum. Additionally, 200 mg/L of ClO 2 significantly induced cell apoptosis of P. digitatum by increasing the fluorescence intensity of the mitochondrial membrane potential from 118 to 1225 and decreased the living cell rate from 96.8 to 6.1%. Further study demonstrated that the content of malondialdehyde and nucleic acid leakage (OD 260 ) of P. digitatum markedly increased, and the mycelial morphology was seriously damaged with increased ClO 2 concentration. These results indicated that ClO 2 could inhibit fungal growth by destroying the membrane integrity of P. digitatum, and the use of ClO 2 may be an alternative strategy to control green mold in postharvest citrus fruits.

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Section: Results and Analysismentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Chlorine Dioxide Controls Green Mold Caused by Penicillium digitatum in Citrus Fruits and the Mechanism Involved

Liu

Jiao

et al. 2020

J. Agric. Food Chem.

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“…The sterilised blade was used to accurately cut a 5 mm diameter rose bengal agar medium, and then, it was inverted into centre of the medium with microcapsules (the equivalent of CA was 1.5MIC) according to the methods reported with modification (Afkhami et al ., 2019; Feng et al ., 2019). It was kept at 28°C for 2 days, and then the treated sample was fixed overnight (4°C) in glutaraldehyde solution (2.5%, v/v).…”

Section: Methodsmentioning

confidence: 99%

Tannic acid modulated the wall compactness of cinnamaldehyde‐loaded microcapsules and enhanced inhibitory effect on Aspergillus brasiliensis

Liu

Wang

Lin

et al. 2022

Int J of Food Sci Tech

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Summary The evaluation of antifungal properties in cinnamaldehyde (CA)‐loaded microcapsules is significant to clarify potential food applications in various food matrices and systems. The impact of tannic acid (TA) crosslinking on the release and the antifungal properties of CA microcapsules was investigated based on gelatin/gum Acacia complex coacervates. The inhibition zone diameter of microencapsulated CA against Aspergillus brasiliensis was dependent on the TA addition and retained 26.1 ± 0.1 mm after 180‐day storage at 25 °C. Specifically, TA enhanced hydrogen bond interactions between the microcapsule wall materials and favoured a sustained release of CA during the incubation with Aspergillus brasiliensis. Meanwhile, confocal laser scanning microscopic observation revealed that the microstructure of multinuclear microcapsules was more compact and intact under the crosslinking of 0.4% TA. The comparation of microscopic morphology and membrane potential of Aspergillus brasiliensis exerted a synergistic antifungal effect between CA and TA crosslinked microcapsules.

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“…A modified method from Feng et al . was used to investigate morphological changes in A. flavus 21 . Aspergillus flavus spore suspensions in PDB were treated with Sub3 at concentrations of 0, 0.1 and 0.15 g L −1 at 28 °C for 6 h. Samples were centrifuged at 8000 × g to obtain spores, then washed twice and resuspended in phosphate‐buffered saline (PBS, 10 mmol L −1 , pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

“…Determination of morphological changes of A. flavus A modified method from Feng et al was used to investigate morphological changes in A. flavus. 21 Aspergillus flavus spore suspensions in PDB were treated with Sub3 at concentrations of 0, 0.1 and 0.15 g L −1 at 28°C for 6 h. Samples were centrifuged at 8000 × g to obtain spores, then washed twice and resuspended in phosphate-buffered saline (PBS, 10 mmol L −1 , pH 7.4). Spores were excited at 488 nm with an argon ion laser, and their positions on forward scatter contour (FSC) versus side scatter contour (SSC) plots were measured using an Accuri C6 flow cytometer (BD Biosciences, San Diego, CA, USA).…”

Section: Antifungal Activity Assaymentioning

confidence: 99%

Sub3 inhibits Aspergillus flavus growth by disrupting mitochondrial energy metabolism, and has potential biocontrol during peanut storage

Zhang

et al. 2020

J Sci Food Agric

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Background: Aspergillus flavus, a saprophytic fungus, is regularly detected in oil-enriched seeds. During colonization, this organism releases aflatoxins that pose a serious risk to food safety and human health. Therefore, an eco-friendly biological approach to inhibit the pathogen is desirable. Results: Experimental results indicated that A. flavus spores could not germinate in potato dextrose broth culture medium, when the concentration of Sub3 exceeded 0.15 g L −1. Morphological evaluation performed by flow cytometry and scanning electron microscopy indicated that spores were shrunken and pitted following Sub3 exposure. Physiological assessment using propidium iodide, 5,5 0 ,6,6 0-tetrachloro-1,1 0 ,3,3 0-tetraethylbenzimidazolocarbocyanine iodide, 2,7-dichlorodihydrofluorescein diacetate and 4 0 ,6-diamidino-2-phenylindole staining revealed damaged cell membranes, decreased mitochondrial membrane potential, increased intracellular reactive oxygen species levels, and elevated large nuclear condensation and DNA fragmentation. Moreover, mitochondrial dehydrogenase activity was reduced by 29.42% and 45.48% after treatment with 0.1 and 0.15 g L −1 Sub3, respectively. Additionally, colonization capacity in peanut was significantly decreased, and the number of spores on seeds treated with Sub3 was decreased by 26.86% (0.1 g L −1) and 77.74% (0.15 g L −1) compared with the control group. Conclusion: Sub3 likely inhibits A. flavus by crossing the cell wall and targeting the cell membrane, disrupting mitochondrial energy metabolism, and inducing DNA damage, leading to spore death. Thus, Sub3 may provide a useful biocontrol strategy to control A. flavus growth in peanuts.

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Antifungal Actions of Glycinin Basic Peptide against Aspergillus niger through the Collaborative Damage to Cell Membrane and Mitochondria

Cited by 13 publications

References 46 publications

Chlorine Dioxide Controls Green Mold Caused by Penicillium digitatum in Citrus Fruits and the Mechanism Involved

Chlorine Dioxide Controls Green Mold Caused by Penicillium digitatum in Citrus Fruits and the Mechanism Involved

Tannic acid modulated the wall compactness of cinnamaldehyde‐loaded microcapsules and enhanced inhibitory effect on Aspergillus brasiliensis

Sub3 inhibits Aspergillus flavus growth by disrupting mitochondrial energy metabolism, and has potential biocontrol during peanut storage

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