Antifungal activities of an endophytic Pseudomonas fluorescens strain Pf1TZ harbouring genes from pyoluteorin and phenazine clusters

Kilani-Feki, Olfa; Khiari, Oussama; Culioli, Gérald; Ortalo-Magné, Annick; Zouari, Nabil; Blache, Yves; Jaoua, Samir

doi:10.1007/s10529-010-0286-9

Cited by 15 publications

(5 citation statements)

References 13 publications

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“…The crude culture supernatants were used as positive controls and the organic solvent as a negative one. After incubation for 2 h at 4°C, the plates were incubated at 27°C for 48 h. The activities were estimated by measuring the inhibition diameter around the wells (Kilani-Feki et al 2010). For each strain, all tests were carried out in triplicate.…”

Section: Antifungal Bioassaysmentioning

confidence: 99%

Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of a Burkholderia cepacia strain collection

Kilani-Feki

Zouari

Culioli

et al. 2011

World J Microbiol Biotechnol

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Twenty epiphytic and rhizospheric bacterial strains harbouring strong antifungal activities were isolated from the Tunisian environment. This group of bacteria was identified as Burkholderia cepacia genomovar I using 16S rDNA and recA fragment gene sequence analyses for two selected strains and RFLP technique for the eighteen other ones. This identification did not show variability between isolates despite the significant differences in the antifungal activities of their culture supernatant and the organic crude extract against Aspergillus niger and other phytopathogenic fungi. Chromatographic and mass spectrometric analyses of these extracts allowed us to confirm the difference between strains of the group. Their metabolic production showed differences in term of contents and quantities of secreted molecules, particularly those which were identified to be involved in the antifungal activities. Two metabolites, named Bc-255 and Bc-257 secreted by the entire group at different amounts, have been purified and tested separately against A. niger. Bc-255 showed an activity twice as high as those shown by Bc-257. The structural characterization of these two compounds by mass spectrometry and nuclear magnetic resonance spectroscopy allowed their identification as two analogous 2-alkylquinolones with only one difference at the alkyl chain.

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Section: Antifungal Bioassaysmentioning

confidence: 99%

Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of a Burkholderia cepacia strain collection

Kilani-Feki

Zouari

Culioli

et al. 2011

World J Microbiol Biotechnol

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“…2F ; see Table S3 in the supplemental material). Pseudomonas is an important biocontrol bacterium that can effectively inhibit Fusarium culmorum ( 24 ), the oomycete Pythium ( 25 ), Fusarium oxysporum , Geotrichum candidum ( 26 ), and other phytopathogenic fungi. Additionally, the proportion of Bacillus in Pt-Cg increased ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities

Zhang

Meng

et al. 2023

Microbiol Spectr

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Our findings revealed that the inoculation of Colletotrichum gloeosporioides has a greater effect on the fungal community than the bacterial community. In addition, coumarins, organic acids, and flavonoids may have recruitment effects on phyllosphere microorganisms, while indoles may have inhibitory effects on these organisms. These findings may provide the theoretical basis for the prevention and control of poplar anthracnose.

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“…Early reports showed the importance of Pseudomonas fluorescens for biological control of take-all disease [ 98 , 99 ]. In this sense, several antagonistic molecules produced by P. fluorescens , such as pyolotuerin, phenazine, HCN, pyrrolnitrin, and 2,4-diacetylphloroglucinol, have been reported [ 100 , 101 ]. However, the early interest in P. fluorescens as a tool for the biological control of plant pathogens focused on examining it as a single strain, rather than using a set of microorganisms associated with the rhizosphere of plants [ 101 ].…”

Section: Ecological Roles Of Microorganisms From Specific Disease-suppressive Soilsmentioning

confidence: 99%

“…In this sense, several antagonistic molecules produced by P. fluorescens , such as pyolotuerin, phenazine, HCN, pyrrolnitrin, and 2,4-diacetylphloroglucinol, have been reported [ 100 , 101 ]. However, the early interest in P. fluorescens as a tool for the biological control of plant pathogens focused on examining it as a single strain, rather than using a set of microorganisms associated with the rhizosphere of plants [ 101 ]. This biocontrol ability was attributed to the production of the antibiotic 2,4-diacetylphloroglicinol (2,4-DAPG) [ 102 , 103 , 104 ].…”

Section: Ecological Roles Of Microorganisms From Specific Disease-suppressive Soilsmentioning

confidence: 99%

Engineering Multigenerational Host-Modulated Microbiota against Soilborne Pathogens in Response to Global Climate Change

Durán

Sadowsky

Viscardi

et al. 2021

Biology

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Crop migration caused by climatic events has favored the emergence of new soilborne diseases, resulting in the colonization of new niches (emerging infectious diseases, EIDs). Soilborne pathogens are extremely persistent in the environment. This is in large part due to their ability to reside in the soil for a long time, even without a host plant, using survival several strategies. In this regard, disease-suppressive soils, characterized by a low disease incidence due to the presence of antagonist microorganisms, can be an excellent opportunity for the study mechanisms of soil-induced immunity, which can be applied in the development of a new generation of bioinoculants. Therefore, here we review the main effects of climate change on crops and pathogens, as well as the potential use of soil-suppressive microbiota as a natural source of biocontrol agents. Based on results of previous studies, we also propose a strategy for the optimization of microbiota assemblages, selected using a host-mediated approach. This process involves an increase in and prevalence of specific taxa during the transition from a conducive to a suppressive soil. This strategy could be used as a model to engineer microbiota assemblages for pathogen suppression, as well as for the reduction of abiotic stresses created due to global climate change.

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Antifungal activities of an endophytic Pseudomonas fluorescens strain Pf1TZ harbouring genes from pyoluteorin and phenazine clusters

Cited by 15 publications

References 13 publications

Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of a Burkholderia cepacia strain collection

Correlation between synthesis variation of 2-alkylquinolones and the antifungal activity of a Burkholderia cepacia strain collection

Effects of Colletotrichum gloeosporioides and Poplar Secondary Metabolites on the Composition of Poplar Phyllosphere Microbial Communities

Engineering Multigenerational Host-Modulated Microbiota against Soilborne Pathogens in Response to Global Climate Change

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