Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian

Wang, Yong; Sun, Yang; Zhang, Ying; Xing, Zhang; Feng, Juntao

doi:10.3390/molecules21060756

Cited by 38 publications

(37 citation statements)

References 39 publications

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“…Interestingly, we found that the color of mycelia in the strains treated with cuminic acid at the EC 50 in PDA plates was visibly lighter than that in control, and mycelia were abnormal by SEM. In addition, the cell membrane permeability and glycerol content were significantly enhanced, which was consistent with the activity of cuminic acid against Phytophthora capsic [ 24 ], which indicated that the mechanism of action of cuminic acid against plant pathogens might be through damaging the mycelial structure and inducing intracellular plasma leakage.…”

Section: Discussionsupporting

confidence: 65%

“…In previous study, cuminic acid and cuminic aldehyde, as the major bioactive constituents of C. cyminum seed, were reported to possess broad spectrum antifungal activities [ 23 , 25 ]. Cuminic acid as a representative chemical of the benzoic acid group, also exhibited a significant antifungal activity and enhanced the defense capacity of plants against Phytophthora capsic [ 24 ]. This study was focused on the biochemistry and physiology alterations in Fusarium oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

“…Fusarium oxysporum f. sp. niveum were collected from infected watermelon plant and maintained on potato dextrose agar (PDA) [ 24 ] medium provided by the Laboratory of Research and Development Center of Biorational Pesticide, Northwest A & F University. Cuminic acid (98%) and carbendazim (98.0%) used in the experiment were purchased from the Sigma Co. (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

“…EC 50 values of cuminic acid against mycelial growth of P. capsic and S. sclerotiorum were only 19.7 µg/mL and 7.3 µg/mL, respectively [ 25 ], which were lower than the EC 50 value of other benzoic acid derivatives in previous reports [ 20 , 21 , 22 ]. In pot experiments, after the application of cuminic acid at 1000 μg/mL, control efficacies of over 60% against P. capsic and S. sclerotiorum were obtained, which was comparable with the efficacy of metalaxyl (250 µg/mL) [ 24 ] and procymidone (100 μg/mL) [ 25 ].…”

Section: Introductionmentioning

confidence: 97%

“…Cuminic acid ( p -isopropylbenzoic acid), isolated from the seed of Cuminum cyminum . L. [ 23 ], belongs to the benzoic acid chemical group [ 24 ]. In a previous study, it has been reported that cuminic acid possessed good inhibition towards several plant pathogens, such as Sclerotinia sclerotiorum , Phytophthora capsici , Rhizoctonia cerealis , and Fusarium oxysporum .…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Activity and Action Mode of Cuminic Acid from the Seeds of Cuminum cyminum L. against Fusarium oxysporum f. sp. Niveum (FON) Causing Fusarium Wilt on Watermelon

et al. 2017

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In order to develop a novel biofungicide, the antifungal activity and action mode of cuminic acid from the seed of Cuminum cyminum L. against Fusarium oxysporum f. sp. niveum (FON) on watermelon was determined systematically. In this study, the median effective concentration (EC50) value for cuminic acid in inhibiting mycelial growth of FON was 22.53 μg/mL. After treatment with cuminic acid, the mycelial morphology was seriously influenced; cell membrane permeability and glycerol content were increased markedly, but pigment and mycotoxin (mainly fusaric acid) were significantly decreased. Synthesis genes of bikaverin (Bike1, Bike2 and Bike3) and fusaric acid (FUB1, FUB2, FUB3 and FUB4) both were downregulated compared with the control, as confirmed by quantitative RT-PCR. In greenhouse experiments, cuminic acid at all concentrations displayed significant bioactivities against FON. Importantly, significant enhancement of activities of SOD, POD, CAT and decrease of MDA content were observed after in vivo cuminic acid treatment on watermelon leaves. These indicated that cuminic acid not only showed high antifungal activity, but also could enhance the self-defense system of the host plant. Above all, cuminic acid showed the potential as a biofungicide to control FON.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Antifungal Activity and Action Mode of Cuminic Acid from the Seeds of Cuminum cyminum L. against Fusarium oxysporum f. sp. Niveum (FON) Causing Fusarium Wilt on Watermelon

et al. 2017

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Gelatin–chitosan–PVA hydrogels and their application in agriculture

López‐Velázquez

Rodríguez‐Rodríguez

Espinosa‐Andrews

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: Hydrogels are materials with great potential in agricultural applications. Biodegradable hydrogels are used as preparations, for example to increase the substratum water capacity, and improve soil structure or agrochemical-controlled release. This work aimed to develop biodegradable hydrogels based on chitosan, gelatin and polyvinyl alcohol (PVA) for releasing inulin isolated from Dahlia tubers to induce protection in chili plants against Phytophthora capsici. The hydrogels were characterized by water absorption capacity, Fourier transform infrared (FTIR), scanning electron microscopy (SEM) and stereomicroscopy microscopy, and degradation capacity using the soil burial test with sterile and inoculated soil. RESULTS: This work demonstrated the ability of a fabrication process in the preparation of gelatin-chitosan-PVA hydrogelsfor potential agricultural applications. The hydrogels showed a dense, tridimensional, interconnected and reticulated structure that was more evident in the hydrogel loaded with inulin. The hydrogels showed a water absorption capacity of ≤12 times its mass. FTIR and light microscopy demonstrated that the hydrogels were biodegradable. The percentage of degradation of hydrogels in inoculated soil was higher than in sterile soil using the soil burial test. Hydrogel loaded with inulin was found to be capable of inducing resistance in chili plants against Phytophthora capsici. CONCLUSION:The hydrogels prepared for the described methodology have great potential for use in the agricultural sector as a reservoir for agrochemicals and inductors in plant resistance treatments. Furthermore, the hydrogels were proven to be biodegradable, offering a promising tool in crop protection. Surface morphology of hydrogelsThe hydrogels have a polyhedral irregular shape. Figure 5(a) shows that the surface morphologies of the hydrogels revealed the formation of a reticulate heterogeneous network. When the hydrogel was loaded with inulin and freeze-dried again, inulin aggregates between the macro channels of the structure [Fig. 5(b)]. Biodegradation test of the hydrogelThe degradation of the hydrogels (H and HI), that were buried in the sterile and inoculated soil were monitored after 7, 14, J Chem Technol Biotechnol 2019; 94: 3495-3504

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Discovery of zeylenone from Uvaria grandiflora as a potential botanical fungicide

Dou

Xie

et al. 2021

Pest Management Science

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BACKGROUND: Botanical pesticides play an important role in organic agricultural practices and are widely used in integrated pest management (IPM). Uvaria grandiflora was mainly reported as traditional medicines and possessed antibacterial, antioxidant, and antiprotozoal activities. Therefore, important biological activities of U. grandiflora may suggest that they have the potential to be used as botanical pesticides.RESULTS: The extract of U. grandiflora exhibited broad-spectrum inhibitory activity toward phytopathogenic fungi and oomycetes, particularly against Colletotrichum musae and Phytophthora capsici, and its secondary metabolite zeylenone also displayed strong antifungal and anti-oomycete activities against phytopathogens. Particularly, half maximal effective concentration (EC 50 ) values of zeylenone against Phytophthora capsici and C. musae were 6.98 and 3.37 ∼g mL −1 , showing better inhibitory effects than those of commercial fungicides (azoxystrobin and osthole). Additionally, the pot experiments showed that the extract of U. grandiflora could effectively control Pseudoperonospora cubensis, Phytophthora infestans, Phytophthora capsici and Podosphaera xanthii. In the field experiment, 5% microemulsion of U. grandiflora extract exhibited 79.72% efficacy against cucumber powdery mildew at 87.5 g ha −1 on the 14th day after two sprayings, which was better than that of 21.5% trifloxystrobin and 21.5% fluopyram SC at 200.9 g ha −1 . Surprisingly, 5% microemulsion of U. grandiflora extract could promote cucumber growth significantly. Furthermore, the action mechanism analysis indicated that zeylenone may damage the cytoderm and affect energy metabolism of Phytophthora capsici.CONCLUSION: It is the first time that the extract of U. grandiflora and zeylenone have been discovered leading to broad application prospects in the development as botanical fungicides.

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Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian

Cited by 38 publications

References 39 publications

Antifungal Activity and Action Mode of Cuminic Acid from the Seeds of Cuminum cyminum L. against Fusarium oxysporum f. sp. Niveum (FON) Causing Fusarium Wilt on Watermelon

Antifungal Activity and Action Mode of Cuminic Acid from the Seeds of Cuminum cyminum L. against Fusarium oxysporum f. sp. Niveum (FON) Causing Fusarium Wilt on Watermelon

Gelatin–chitosan–PVA hydrogels and their application in agriculture

Discovery of zeylenone from Uvaria grandiflora as a potential botanical fungicide

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