Antifungal activity and genomic characterization of the biocontrol agent Bacillus velezensis CMRP 4489

Baptista, Júlia; Teixeira, Gustavo Manoel; Jesus, Maria Luiza Abreu de; Bertê, Rosiana; Higashi, Allan Yukio; Mosela, Mirela; Silva, Daniel Vieira da; Oliveira, João Paulo de; Sanches, Danilo Sipoli; Brancher, Jacques Duílio; Balbi-Peña, María Isabel; Pereira, Ulisses de Pádua; Oliveira, Admilton Gonçalves de

doi:10.1038/s41598-022-22380-0

Cited by 17 publications

(4 citation statements)

References 63 publications

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“…There are some studies on the effect of biocontrol strain on pathogens, such as the ability of B. subtilis Y2 to inhibit the growth of Alternaria brassicicola in pear fruit [41]. The antagonistic activity of B. velezensis CMRP 4489 could inhibit the growth of pathogens by the formation of biofilm [42].…”

Section: Discussionmentioning

confidence: 99%

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Zheng

Sun

et al. 2023

JoF

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Bacillus mojavensis D50, a biocontrol strain, is used to prevent and treat the fungal plant pathogen Botrytis cinerea. Bacillus mojavensis D50’s biofilms can affect its colonization; thus, the effects of different metal ions and culture conditions on biofilm formation were determined in this study. The results of medium optimization showed that Ca2+ had the best ability to promote biofilm formation. The optimal medium composition for the formation of biofilms contained tryptone (10 g/L), CaCl2 (5.14 g/L), and yeast extract (5.0 g/L), and the optimal fermentation conditions included pH 7, a temperature of 31.4 °C, and a culture time of 51.8 h. We found that the antifungal activity and abilities to form biofilms and colonize roots were improved after optimization. In addition, the levels of expression of the genes luxS, SinR, FlhA, and tasA were up-regulated by 37.56-, 2.87-, 12.46-, and 6.22-fold, respectively. The soil enzymatic activities which related biocontrol-related enzymes were the highest when the soil was treated by strain D50 after optimization. In vivo biocontrol assays indicated that the biocontrol effect of strain D50 after optimization was improved.

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Section: Discussionmentioning

confidence: 99%

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Zheng

Sun

et al. 2023

JoF

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“…Auxin is an important factor in phosphorus regulation [160]. PSB can also help Solanaceae resist pathogens after being soaked in bacterial fertilizers, such as reducing Fusarium oxysporum [161], Aspergillus, Penicillium [162], and Botrytis cinerea by 48% [163], as well as Macropomina phaseolina [164] and Pseudomonas aeruginosa [165], and this may be related to the discovery of antibiotic-resistance genes in PSB [166]. Studies have shown that under lowphosphorus stress, bacteria are more sensitive to phosphorus enrichment and recruitment than fungi [167].…”

Section: Colonization Of Bacterial Communities In Plants Is Influence...mentioning

confidence: 99%

Influence and Role of Fungi, Bacteria, and Mixed Microbial Populations on Phosphorus Acquisition in Plants

Luo,

Ma,

Feng

et al. 2024

Agriculture

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Phosphorus (P) stands as a pivotal macroelement in relation to the growth of plants. It plays a significant role in physiological processes, as components of biofilms and nucleotides, and in metabolic activities within plants. The deprivation of phosphorus detrimentally impacts the growth and developmental of plants. However, the rhizosphere’s beneficial fungi and bacteria augment the efficacy of phosphorus uptake, participate in the molecular regulation of phosphorus, stimulate physiological alterations in plants, and facilitate signal transmission. In order to give readers a better understanding of the effects and positive roles of soil beneficial fungi and bacteria in regulating plant phosphorus acquisition and transport, this present review introduces the role and influence of rhizosphere microorganisms (fungi and bacteria) in assisting plant phosphorus absorption, and summarizes the key phosphorus transporters found in their interaction with plants. Using mixed microbial populations as composite microbial fertilizers has a positive effect on plants under phosphorus-deficiency conditions. It will be conducive to a better understanding of the mutualistic relationship between fungi, bacteria, and plants to provide a way to reduce the application of phosphorus fertilizers efficiently, and to provide a research background for the development of microbiological fertilizers.

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“…The complete genome was then deposited in DDBJ / EMBL / GenBank under the accession number CP023748 (Baptista et al, 2018). Additionally, LABIM40 was deposited as strain CMRP 4489 in the Coleções Microbiológicas da Rede Paranaense (CMRP) network at the Federal University of Paraná, in Curitiba, Brazil (Baptista et al, 2022). Prior to each experiment, the LABIM40 strain was activated from the cryopreserved stock culture on nutrient agar (NA) plates and incubated at 30 °C with a 12 hour photoperiod until colony formation occurred.…”

Section: Isolation and Identification Of B Velezensis Labim40 Strainmentioning

confidence: 99%

In vitro control of phytopathogenic fungi and damping-off of tomato by Bacillus velezensis LABIM40 (CMRP 4489)

Almeida,

Baptista,

Higashi

et al. 2023

Sem. Ci. Agr.

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The in vitro antagonistic activity of Bacillus velezensis LABIM40 (strain CMRP 4489) was assessed against Alternaria linariae, Botryotinia squamosa, Colletotrichum lindemuthianum, Gibberella zeae, and Rhizoctonia solani. An experiment was conducted using treated seeds under growth chamber conditions to determine the impact of various LABIM40 formulations on tomato seedling growth and the biocontrol of damping-off caused by R. solani. The treatments included the use of LABIM40 cell suspension, LABIM40 cell-free supernatant (CFS), 10 times concentrated CFS (10× CFS), commercial products based on Bacillus amyloliquefaciens (CP_1) and Bacillus subtilis (CP_2), and water. The effects of these products were assessed on tomato seedlings grown in sterile substrate or substrate inoculated with R. solani. In a dual culture test, B. velezensis LABIM40 inhibited the mycelial growth of the aforementioned fungal pathogens by 46.6%, 67.4%, 64.7%, 49.0%, and 54.4%, respectively. The minimum inhibitory concentration against each fungus was determined using varying concentrations of CFS in potato dextrose agar medium, followed by a regression analysis of mycelial growth inhibition. Except for A. linariae, the logarithmic model provided the best fit in all cases. Tomato seedlings from seeds treated with 10× CFS in inoculated substrate exhibited a survival rate 57% higher than that exhibited by the control treatment. However, no growth promotion was observed in tomato plants from seeds treated with LABIM40 cells or its CFS metabolites. In summary, these findings highlight the antagonistic activity of B. velezensis LABIM40 against A. linariae, B. squamosa, C. lindemuthianum, G. zeae, and R. solani, as demonstrated by dual culture and CFS diffusion tests. This suggests its potential as a biocontrol agent for damping-off in tomatoes.

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Antifungal activity and genomic characterization of the biocontrol agent Bacillus velezensis CMRP 4489

Cited by 17 publications

References 63 publications

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Influence and Role of Fungi, Bacteria, and Mixed Microbial Populations on Phosphorus Acquisition in Plants

In vitro control of phytopathogenic fungi and damping-off of tomato by Bacillus velezensis LABIM40 (CMRP 4489)

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