Antifungal activity and patterns of N -acetyl-chitooligosaccharide degradation via chitinase produced from Serratia marcescens PRNK-1

Moon, Chaeyeong; Seo, Dong‐Jun; Song, Yuanlin; Hong, Sin-Hyoung; Choi, Seung-Hee; Jung, Woo‐Jin

doi:10.1016/j.micpath.2017.10.039

Cited by 32 publications

(26 citation statements)

References 33 publications

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“…Colloidal chitin was widely used as an inducer for the production of chitinase in various studies. For example, the expression of chitinases from Serratia marcescens PRNK-1 [ 18 ], Pedobacter sp. PR-M6 [ 19 ], and Bacillus licheniformis B307 [ 20 ] were incubated in 0.5% (w/v) colloidal chitin.…”

Section: Resultsmentioning

confidence: 99%

A Broad-Specificity Chitinase from Penicillium oxalicum k10 Exhibits Antifungal Activity and Biodegradation Properties of Chitin

Yan

Peng

et al. 2021

Marine Drugs

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Penicillium oxalicum k10 isolated from soil revealed the hydrolyzing ability of shrimp chitin and antifungal activity against Sclerotinia sclerotiorum. The k10 chitinase was produced from a powder chitin-containing medium and purified by ammonium sulfate precipitation and column chromatography. The purified chitinase showed maximal activity toward colloidal chitin at pH 5 and 40 °C. The enzymatic activity was enhanced by potassium and zinc, and it was inhibited by silver, iron, and copper. The chitinase could convert colloidal chitin to N-acetylglucosamine (GlcNAc), (GlcNAc)2, and (GlcNAc)3, showing that this enzyme had endocleavage and exocleavage activities. In addition, the chitinase prevented the mycelial growth of the phytopathogenic fungi S. sclerotiorum and Mucor circinelloides. These results indicate that k10 is a potential candidate for producing chitinase that could be useful for generating chitooligosaccharides from chitinous waste and functions as a fungicide.

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Section: Resultsmentioning

confidence: 99%

A Broad-Specificity Chitinase from Penicillium oxalicum k10 Exhibits Antifungal Activity and Biodegradation Properties of Chitin

Yan

Peng

et al. 2021

Marine Drugs

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“…Prolonged incubation (up to 240–300 min) resulted in a further increase in (GlcNAc) concentration. Another study on chitinase was conducted by Moon et al (2017). They used partially purified chitinase from Serratia marcescens to investigate the patterns of degradation of (GlcNAc) 2−4 .…”

Section: Enzymatic Modification Of Chitin and Chitosanmentioning

confidence: 99%

Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides

Kaczmarek

Struszczyk‐Świta

et al. 2019

Front. Bioeng. Biotechnol.

288

157

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Chitin and its N-deacetylated derivative chitosan are two biological polymers that have found numerous applications in recent years, but their further deployment suffers from limitations in obtaining a defined structure of the polymers using traditional conversion methods. The disadvantages of the currently used industrial methods of chitosan manufacturing and the increasing demand for a broad range of novel chitosan oligosaccharides (COS) with a fully defined architecture increase interest in chitin and chitosan-modifying enzymes. Enzymes such as chitinases, chitosanases, chitin deacetylases, and recently discovered lytic polysaccharide monooxygenases had attracted considerable interest in recent years. These proteins are already useful tools toward the biotechnological transformation of chitin into chitosan and chitooligosaccharides, especially when a controlled non-degradative and well-defined process is required. This review describes traditional and novel enzymatic methods of modification of chitin and its derivatives. Recent advances in chitin processing, discovery of increasing number of new, well-characterized enzymes and development of genetic engineering methods result in rapid expansion of the field. Enzymatic modification of chitin and chitosan may soon become competitive to conventional conversion methods.

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“…The role played by chitinases in the metabolism of S. marcescens is still unclear, but they were detected in RPA1 as well as in RPH1. Partially purified chitinases of S. marcescens PRNK-1, an isolated from cockroach, strongly inhibited the fungal growth of Rhizoctonia solani and Fusarium oxysporum in vitro (Moon et al, 2017). Chitinases secreted in the TDT could be especially important to protect its microbiota from infection by entomopathogenic fungi, i.e., Beauveria bassiana, Gliocladium virens, Metarhizium anisopliae , and Isaria fumosorosea (Vázquez-Martínez et al, 2014; Forlani et al, 2015; Huang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract

et al. 2019

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Serratia marcescens is a bacterium with the ability to colonize several niches, including some eukaryotic hosts. S. marcescens have been recently found in the gut of hematophagous insects that act as parasite vectors, such as Anopheles, Rhodnius, and Triatoma. While some S. marcescens strains have been reported as symbiotic or pathogenic to other insects, the role of S. marcescens populations from the gut microbiota of Rhodnius prolixus, a vector of Chagas’ disease, remains unknown. Bacterial colonies from R. prolixus gut were isolated on BHI agar. After BOX-PCR fingerprinting, the genomic sequences of two isolates RPA1 and RPH1 were compared to others S. marcescens from the NCBI database in other to estimate their evolutionary divergence. The in vitro trypanolytic activity of these two bacterial isolates against Trypanosoma cruzi (DM28c clone and Y strain) was assessed by microscopy. In addition, the gene expression of type VI secretion system (T6SS) was detected in vivo by RT-PCR. Comparative genomics of RPA1 and RPH1 revealed, besides plasmid presence and genomic islands, genes related to motility, attachment, and quorum sensing in both genomes while genes for urea hydrolysis and type II secretion system (T2SS) were found only in the RPA1 genome. The in vitro trypanolytic activity of both S. marcescens strains was stronger in their stationary phases of growth than in their exponential ones, with 65–70 and 85–90% of epimastigotes (Dm28c clone and Y strain, respectively) being lysed after incubation with RPA1 or RPH1 in stationary phase. Although T6SS transcripts were detected in guts up to 40 days after feeding (DAF), R. prolixus morbidity or mortality did not appear to be affected. In this report, we made available two trypanolytic S. marcescens strains from R. prolixus gut to the scientific community together with their genomic sequences. Here, we describe their genomic features with the purpose of bringing new insights into the S. marcescens adaptations for colonization of the specific niche of triatomine guts. This study provides the basis for a better understanding of the role of S. marcescens in the microbiota of R. prolixus gut as a potential antagonist of T. cruzi in this complex system.

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Antifungal activity and patterns of N -acetyl-chitooligosaccharide degradation via chitinase produced from Serratia marcescens PRNK-1

Cited by 32 publications

References 33 publications

A Broad-Specificity Chitinase from Penicillium oxalicum k10 Exhibits Antifungal Activity and Biodegradation Properties of Chitin

A Broad-Specificity Chitinase from Penicillium oxalicum k10 Exhibits Antifungal Activity and Biodegradation Properties of Chitin

Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides

In vitro Trypanocidal Activity, Genomic Analysis of Isolates, and in vivo Transcription of Type VI Secretion System of Serratia marcescens Belonging to the Microbiota of Rhodnius prolixus Digestive Tract

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