Antifungal Activity of a Bowman–Birk‐type Trypsin Inhibitor from Wheat Kernel

Chilosi, G.; Caruso, Carla; Caporale, Carlo; Leonardi, Luca; Bertini, Laura; Buzi, Adva; Nobile, Monica; Magro, P.; Buonocore, Vincenzo

doi:10.1046/j.1439-0434.2000.00527.x

Cited by 70 publications

(40 citation statements)

References 27 publications

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“…The results of the study of Chen et al (25) revealed that the resistance of certain corn genotypes to fungal infection might be related to the action of trypsin inhibitor in lowering the production of fungal ␣-amylase and its activity and consequently reducing the availability of simple sugars for fungal growth. A trypsin inhibitor from wheat kernel also elicited a potent antifungal BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS effect (26). A 28-kDa alkaline protease inhibitor from Streptomyces (27), a 24-kDa cysteine protease inhibitor from pearl millet (28), and the HIV-1 protease inhibitors (29) exhibited antifungal activity.…”

Section: Discussionmentioning

confidence: 99%

A Bowman–Birk-Type Trypsin-Chymotrypsin Inhibitor from Broad Beans

Rao

2001

Biochemical and Biophysical Research Communications

112

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Section: Discussionmentioning

confidence: 99%

A Bowman–Birk-Type Trypsin-Chymotrypsin Inhibitor from Broad Beans

Rao

2001

Biochemical and Biophysical Research Communications

112

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“…Another three genes induced by aluminum in wheat (wali3, wali5 and wali6) have also been found to be similar to some sugarcane genes. These wheat genes encode Bowman-Birk type trypsin inhibitors, an extensively studied family of protease inhibitors (Laskowsky and Kato, 1980), whose homologs in soybean have been shown to have antifungal activity (Chilosi et al, 2000) and have been suggested to be a defense against insect feeding (Belzunces et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Prospecting sugarcane genes involved in aluminum tolerance

et al. 2001

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Aluminum is one of the major factors that affect plant development in acid soils, causing a substantial reduction in yield in many crops. In South America, about 66% of the land surface is made up of acid soils where high aluminum saturation is one of the main limiting factors for agriculture. The biochemical and molecular basis of aluminum tolerance in plants is far from being completely understood despite a growing number of studies, and in the specific case of sugarcane there are virtually no reports on the effects of gene regulation on aluminum stress. The objective of the work presented in this paper was to prospect the sugarcane expressed sequence tag (SUCEST) data bank for sugarcane genes related to several biochemical pathways known to be involved in the responses to aluminum toxicity in other plant species and yeast. Sugarcane genes similar to most of these genes were found, including those coding for enzymes that alleviate oxidative stress or combat infection by pathogens and those which code for proteins responsible for the release of organic acids and signal transducers. The role of these genes in aluminum tolerance mechanisms is reviewed. Due to the high level of genomic conservation in related grasses such as maize, barley, sorghum and sugarcane, these genes may be valuable tools which will help us to better understand and to manipulate aluminum tolerance in these species.

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“…3 BowmaneBirk inhibitors (BBIs) typically comprise 60e90 amino acids, and perform dual inhibition of trypsin-and/or chymotrypsin-like proteases via disulfide-bonded b-hairpin loop motifs, which bind at catalytic sites via canonical protease recognition sequences. 4,5 In the early 1990s it was demonstrated that synthetic peptide mimics of the BBI b-hairpin loop motif retain biological activity.…”

Section: Introductionmentioning

confidence: 99%

Sunflower trypsin inhibitor (SFTI-1) analogues of synthetic and biological origin via N→S acyl transfer: potential inhibitors of human Kallikrein-5 (KLK5)

Shariff¹,

Zhu²,

Cowper³

et al. 2014

Tetrahedron

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a b s t r a c tSunflower Trypsin Inhibitor (SFTI-1) analogues have been prepared from simple linear precursors produced either by chemical synthesis or following purification from Escherichia coli. We have shown, for the first time that these linear SFTI-1 derived peptide sequences can be converted to circular peptides via selective consecutive acyl transfer reactions, and that the products derived from synthetic and bacterial origin are identical. Preliminary analysis of the semi-synthetic SFTI-1 analogues confirmed SFTI-I10H as an inhibitor of Kallikrein-5 (KLK5) protease that could also mediate its action on human keratinocytes. The preliminary results obtained serve as a useful starting point for the biological production of SFTI-1 based, selective KLK5 inhibitors for the treatment of atopic dermatitis.

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Antifungal Activity of a Bowman–Birk‐type Trypsin Inhibitor from Wheat Kernel

Cited by 70 publications

References 27 publications

A Bowman–Birk-Type Trypsin-Chymotrypsin Inhibitor from Broad Beans

A Bowman–Birk-Type Trypsin-Chymotrypsin Inhibitor from Broad Beans

Prospecting sugarcane genes involved in aluminum tolerance

Sunflower trypsin inhibitor (SFTI-1) analogues of synthetic and biological origin via N→S acyl transfer: potential inhibitors of human Kallikrein-5 (KLK5)

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