Antifungal activity of Bacillus mojavensis D50 against Botrytis cinerea causing postharvest gray mold of tomato

Zheng, Lining; Gu, Xuehu; Xiao, Yue; Wang, Shengyi; Liu, Ling; Pan, Hongyu; Zhang, Hao

doi:10.1016/j.scienta.2023.111841

Cited by 17 publications

(2 citation statements)

References 44 publications

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“…Currently, the primary way to control pathogens is the use of chemical pesticides [5]. They are inexpensive and highly efficient.…”

Section: Introductionmentioning

confidence: 99%

“…mojavensis D50, which was isolated from tomato rhizosphere soil, has been reported to have a substantial effect on B. cinerea. Moreover, its fermentation supernatant also had great antifungal effect on tomato gray mold [5]. However, there have been few studies on the promotion of biofilm formation and biocontrol effects by the manipulation of concentrations of metal ions and culture conditions.…”

Section: Introductionmentioning

confidence: 99%

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Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Zheng

Sun

et al. 2023

JoF

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Bacillus mojavensis D50, a biocontrol strain, is used to prevent and treat the fungal plant pathogen Botrytis cinerea. Bacillus mojavensis D50’s biofilms can affect its colonization; thus, the effects of different metal ions and culture conditions on biofilm formation were determined in this study. The results of medium optimization showed that Ca2+ had the best ability to promote biofilm formation. The optimal medium composition for the formation of biofilms contained tryptone (10 g/L), CaCl2 (5.14 g/L), and yeast extract (5.0 g/L), and the optimal fermentation conditions included pH 7, a temperature of 31.4 °C, and a culture time of 51.8 h. We found that the antifungal activity and abilities to form biofilms and colonize roots were improved after optimization. In addition, the levels of expression of the genes luxS, SinR, FlhA, and tasA were up-regulated by 37.56-, 2.87-, 12.46-, and 6.22-fold, respectively. The soil enzymatic activities which related biocontrol-related enzymes were the highest when the soil was treated by strain D50 after optimization. In vivo biocontrol assays indicated that the biocontrol effect of strain D50 after optimization was improved.

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“…Currently, the primary way to control pathogens is the use of chemical pesticides [5]. They are inexpensive and highly efficient.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Zheng

Sun

et al. 2023

JoF

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Caffeic acid improves biocontrol effect of Pantoea jilinensis D25 against tomato gray mold

Zheng,

Wang

et al. 2024

Pest Management Science

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BACKGROUNDGray mold in tomato caused by Botrytis cinerea is a destructive disease, which can be treated using biocontrol agents. Pretreatment of biocontrol strains with oxidative‐stress‐ameliorating compounds can enhance their tolerance to oxidative microenvironment in infected fruit wounds. In this study, we aimed to determine the effect of caffeic acid (CA) on the biocontrol efficacy of Pantoea jilinensis D25 in gray mold in cherry tomato.RESULTSD25 strain pretreated with CA exhibited enhanced oxidative stress response in cherry tomato wounds. CA‐treated D25 cells recovered from cherry tomato wounds had lower ROS level and cellular oxidative damage than D25 cells not treated with CA. The antioxidation‐related genes (CAT and GPX) were upregulated and colonization ability of D25 strain was improved in cherry tomato wounds after CA application. Furthermore, the biocontrol effects of CA‐treated D25 strain were improved. CA pretreatment to D25 strain could significantly improve the expression of defense‐related genes (SlMYC‐2, SlLoxD, SlEIN2, and SlEIN3) and activity of defense‐related enzymes in cherry tomato wounds.CONCLUSIONApplication of CA to D25 strain could improve its ability to combat antioxidant stress and biocontrol efficacy against postharvest tomato gray mold. © 2024 Society of Chemical Industry.

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Design, synthesis, molecular docking and antimicrobial evaluation of benzoylurea derivatives containing difluoromethyl (trifluoromethyl) pyrimidine

Wu,

An,

Lan

et al. 2024

Pest Management Science

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BACKGROUNDThe reduction in agricultural product quality and yield caused by fungal and bacterial plant diseases has led to considerable economic losses in global crop production and poses a threat to human health. The primary method of control remains the use of chemical agents. In an effort to develop novel and highly effective antimicrobial agents, a series of benzoylurea derivatives incorporating a difluoromethyl (trifluoromethyl) pyrimidine structure were designed and synthesized.RESULTSIn this study, we designed and synthesized a series of novel benzoylurea derivatives containing difluoromethyl (trifluoromethyl) pyrimidine fragments. Several of the synthesized compounds exhibited notable antifungal activity in vitro against PS, CBC, BBC and TBC. Their efficacy surpassed that of the positive controls HM and Pyr. Notably, 6s demonstrated an EC50 value of 4.10 μg mL−1, significantly lower than the 31.25 μg mL−1 for Pyr. In antibacterial assays, 6s also showed an 87.49% inhibition rate against Xoc. Moreover, in vivo tests against CBC revealed a protective efficacy of 59.39% at a concentration of 25 μg mL−1. Molecular docking simulations further supported its strong activity. To explore the mechanism of action of 6s on CBC, we conducted scanning electron microscopy, succinate dehydrogenase enzyme assays, and measurements of dry weight, membrane permeability, cellular contents, and ROS.CONCLUSIONThis study underscores the potential of benzoylurea derivatives containing difluoromethyl (trifluoromethyl) pyrimidine fragments as lead compounds for the management of CBC. The results offer important insights and pave the way for the development of novel fungicides, contributing to improved crop protection strategies in agriculture. © 2024 Society of Chemical Industry.

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Antifungal activity of Bacillus mojavensis D50 against Botrytis cinerea causing postharvest gray mold of tomato

Cited by 17 publications

References 44 publications

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Caffeic acid improves biocontrol effect of Pantoea jilinensis D25 against tomato gray mold

Design, synthesis, molecular docking and antimicrobial evaluation of benzoylurea derivatives containing difluoromethyl (trifluoromethyl) pyrimidine

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