Antifungal Activity of Bacterial Strains from Maize Silks against Fusarium Verticillioides

Diniz, Gisele de Fátima Dias; Cota, L. V.; Figueiredo, J. E. F.; Aguiar, F. M.; Silva, Dagma Dionísia da; Lana, U. G. de P.; Santos, Vera Lúcia dos; Marriel, I. E.; Paiva, C. A. O.

doi:10.21203/rs.3.rs-469163/v1

Cited by 3 publications

(4 citation statements)

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“…In another study, Diniz et al (2021), analyzing the in vitro antifungal activity of four endophytic (ISD04 and IPR45) and epiphytic (CT02 and IM14) bacterial strains from maize silks, found inhibition values from 58.5 to 100%. In addition, the four isolates changed the hyphae morphology and inhibited the conidial germination by up to 100% (strain IPR45).…”

Section: Discussionmentioning

confidence: 99%

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Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides

et al. 2022

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The mycotoxigenic fungus Fusarium verticillioides is the primary maize pathogen and causes the maize stalk and ear rot diseases with significant economic losses. Furthermore, the excessive use of fungicides to control F. verticillioides constitutes threats to the environment and human health. Thus, sustainable alternatives such as biological control are needed to minimize the hazards associated with the current method. Although much is known about the vulnerability of the maize silks as a gateway for several fungal pathogens invading the developing grains, studies on the chemical properties of silk extracts and their resident microbiota are scarce. This study isolated and characterized bacteria and fungi that colonize the maize stigma to assess new potential biocontrol agents. The samples were collected from maize fields in the Brazilian localities of Sete Lagoas-MG, Sidrolândia-MS, Sertaneja-PR, and Goiânia-GO. One hundred sixty-seven microorganisms were isolated, 46% endophytic and 54% epiphytic. First, the antagonist activity was evaluated by the agar disc diffusion method performed in triplicate, and 83% of the isolates showed antagonist activity against F. verticillioides. Then, the 42 most efficient isolates were identified based on the partial sequencing of the bacterial 16S rRNA gene and fungi ITS region. The bacteria belong to the genera Bacillus (57.1%), Burkholderia (23.8%), Achromobacter (7.1%), Pseudomonas (2.4%), and Serratia (2.4%), while the fungi are Penicillium (2.4%), Candida (2.4), and Aspergillus (2.4%). The results showed that microorganisms from maize stigma might represent new promising agents for F. verticillioides control.

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Section: Discussionmentioning

confidence: 99%

“…In another study, Diniz et al (2021) tested four endophytic and epiphytic bacteria from maize silks in vitro experimental plots), Sidrolândia-MS (field maize), Goiânia-GO (Embrapa Rice and bean, experimental plots) and Sertaneja-PR (field maize) in the 2016 harvest were randomly collected.…”

Section: Introductionmentioning

confidence: 99%

Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides

et al. 2022

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“…Beneficial microbes can interact with pathogens directly or indirectly by inducing host resistance [43][44][45]. A recent study reported culturing of bacteria from maize silks in Brazil and found promising candidates for biocontrol of Fusarium verticillioides stalk rot and stored grains [46,47]. This study sampled environmentally exposed silk tissue from cobs randomly collected from fields.…”

Section: Introductionmentioning

confidence: 99%

The Cultured Microbiome of Pollinated Maize Silks Shifts after Infection with Fusarium graminearum and Varies by Distance from the Site of Pathogen Inoculation

Thompson,

Shrestha,

Rinne

et al. 2023

Pathogens

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Styles transmit pollen-derived sperm nuclei from pollen to ovules, but also transmit environmental pathogens. The microbiomes of styles are likely important for reproduction/disease, yet few studies exist. Whether style microbiome compositions are spatially responsive to pathogens is unknown. The maize pathogen Fusarium graminearum enters developing grain through the style (silk). We hypothesized that F. graminearum treatment shifts the cultured transmitting silk microbiome (TSM) compared to healthy silks in a distance-dependent manner. Another objective of the study was to culture microbes for future application. Bacteria were cultured from husk-covered silks of 14 F. graminearum-treated diverse maize genotypes, proximal (tip) and distal (base) to the F. graminearum inoculation site. Long-read 16S sequences from 398 isolates spanned 35 genera, 71 species, and 238 OTUs. More bacteria were cultured from F. graminearum-inoculated tips (271 isolates) versus base (127 isolates); healthy silks were balanced. F. graminearum caused a collapse in diversity of ~20–25% across multiple taxonomic levels. Some species were cultured exclusively or, more often, from F. graminearum-treated silks (e.g., Delftia acidovorans, Klebsiella aerogenes, K. grimontii, Pantoea ananatis, Stenotrophomonas pavanii). Overall, the results suggest that F. graminearum alters the TSM in a distance-dependent manner. Many isolates matched taxa that were previously identified using V4-MiSeq (core and F. graminearum-induced), but long-read sequencing clarified the taxonomy and uncovered greater diversity than was initially predicted (e.g., within Pantoea). These isolates represent the first comprehensive cultured collection from pathogen-treated maize silks to facilitate biocontrol efforts and microbial marker-assisted breeding.

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“…There are many kinds of active substances that can eradicate fungi, including antifungal proteins (Chlebek et al, 2020), polypeptides (Li et al, 2021), phenolic weight fungal cell wall hydrolases or oxidases. Secretion of antifungal proteins is one of the important mechanisms by which bacteria kill the infected fungi, and antifungal proteins also constitute the basis of biocontrol of fungal diseases and plant antifungal genetic engineering (Diniz et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Identification of postharvest fruit biocontrol strain Burkholderia contaminans against fungi decay

Shi¹,

Du²

2023

Europ.J.Hortic.Sci.

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Botrytis cinerea (gray mold) infection can causepostharvest quality decline of various fruits, resulting in huge economic losses. The use of fungicides to maintain fruit quality will lead to potential environmental pollution, harming human health. successfully applied to control postharvest diseases of fruits. In the present study, bacterial strain B-1 with a high antifungal activity against Botrytis cinerea was isolated from the surface of apricots. Combining morphological and biochemical characterization with 16S rDNA and recA gene sequencing, this bacterial Burkholderia contaminans, which is a kind of B. cepacia complex (Bcc). In order to further reveal its antifungal mechanism, the bacterial cells were lysed, and a protein with antifungal activity was isolated by DEAE-52 ionexchange chromatography and sephadex G-100 gel a molecular weight of 17.5 kD was analyzed by mass spectrometry and retrieved from ncbiprot database, and was determined to be as superoxide dismutase.The bacterial protein could effectively inhibit mycelia growth of B. cinerea, Penicillium expansum, and Alternaria tenuissima in vitro. The protein exhibited a broad-spectrum antifungal activity against nine pathogenic fungi. Moreover, it could cinee mildew in grape and strawberry fruits. After six days of inoculation with antifungal crude protein, the inhibition rates of grape and strawberry fruits were 88.97% and 86.55%, respectively. In addition, it could affect the DNA level, reactive oxygen species and mitochondrial membrane potential in pathogenic fungi. This study demonstrated that the B. contaminans B-1 strain had a great potential to be used a biocontrol microorganism to protect against postharvest fruit fungal decay.

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Antifungal Activity of Bacterial Strains from Maize Silks against Fusarium Verticillioides

Cited by 3 publications

References 47 publications

Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides

Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides

The Cultured Microbiome of Pollinated Maize Silks Shifts after Infection with Fusarium graminearum and Varies by Distance from the Site of Pathogen Inoculation

Identification of postharvest fruit biocontrol strain Burkholderia contaminans against fungi decay

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