Antifungal Activity of Bioactive Compounds Produced by the Endophytic Fungus Paecilomyces sp. (JN227071.1) against Rhizoctonia solani

Hawar, Sumaiya Naeema; Taha, Zainab K.; Hamied, Atyaf Saied; Al-Shmgani, Hanady S.; Sulaiman, Ghassan M.; Elsilk, Sobhy E.

doi:10.1155/2023/2411555

Cited by 12 publications

(2 citation statements)

References 53 publications

(64 reference statements)

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“…Propenoic acid, tridecyl ester, a bioactive molecule produced by Paecilomyces sp. (JN227071.1) endophytic fungus reported to exhibit antifungal action against Rhizoctonia solani (Hawar et al, 2023). As a result, the current study focuses on the antibacterial properties of Xenomyrothecium tongaense to battle multi-drug-resistant bacterial infections.…”

Section: Discussionmentioning

confidence: 99%

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Sundar,

Arunachalam

2024

Front. Microbiol.

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IntroductionEndophytes refer to microorganisms residing within the endosphere of plants, particularly perennials, without inflicting noticeable injury or inducing obvious morphological variations to their host plant or host organism. Endophytic fungi, although often overlooked microorganisms, have garnered interest due to their significant biological diversity and ability to produce novel pharmacological substances.MethodsIn this study, fourteen endophytic fungi retrieved were from the stem of the perennial plant Polianthes tuberosa of the Asparagaceae family. These fungal crude metabolites were tested for antagonistic susceptibility to Multi-Drug Resistant (MDR) pathogens using agar well diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) assays. The chequerboard test was used to assess the synergistic impact of active extract.Results and discussionIn early antibacterial screening using the Agar plug diffusion test, three of fourteen endophytes demonstrated antagonism against Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin-resistant Enterococcus (VRE). Three isolates were grown in liquid medium and their secondary metabolites were recovered using various organic solvents. Eight extracts from three endophytic fungi displayed antagonism against one or more human pathogens with diameters ranging from 11 to 24 mm. The highest antagonistic effect was obtained in ethyl acetate extract for PTS8 isolate against two MRSA (ATCC 43300, 700699) with 20 ± 0.27 and 22 ± 0.47 mm zones of inhibition, respectively, among different solvent extracts. The extract had MICs of 3.12 ± 0.05 and 1.56 ± 0.05 μg/mL, and MBCs of 50 ± 0.01 and 12.5 ± 0.04 μg/mL, respectively. Antagonism against VRE was 18 ± 0.23 mm Zone of Inhibition (ZOI) with MIC and MBC of 6.25 ± 0.25 and 25 ± 0.01 μg/mL. When ethyl acetate extract was coupled with antibiotics, the chequerboard assay demonstrated a synergistic impact against MDR bacteria. In an antioxidant test, it had an inhibitory impact of 87 ± 0.5% and 88.5 ± 0.5% in 2,2-Diphenyl-1-Picrylhydrazyl and reducing power assay, respectively, at 150 μg/mL concentration. PTS8 was identified as a Xenomyrothecium tongaense strain by 18S rRNA internal transcribed spacer (ITS) sequencing. To our insight, it is the foremost study to demonstrate the presence of an X. tongaense endophyte in the stem of P. tuberosa and the first report to study the antibacterial efficacy of X. tongaense which might serve as a powerful antibacterial source against antibiotic-resistant human infections.

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Section: Discussionmentioning

confidence: 99%

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Sundar,

Arunachalam

2024

Front. Microbiol.

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“…Biologically active natural compounds are molecules produced by plants or plant-related microbes, such as endophytes [18,19]. In recent times, plant extracts have garnered signifcant attention for their potential medicinal applications due to their diverse phytochemical composition and associated health benefts.…”

Section: Introductionmentioning

confidence: 99%

Impact of Grinding and Sorting Particle Size on Phytochemical Yield in Dipterocarpus alatus Leaf Extract

Senawong,

Katekaew,

Juntahum

et al. 2023

International Journal of Biomaterials

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The main objective of this study was to investigate the impact of grinding (pretreatment) with a pin mill on the crude extract yields of Dipterocarpus alatus (Yang-Na) leaves. A factorial design in a completely randomized design was conducted to study the combinational effects of sieve sizes (1.0, 1.5, and 3.0 mm) and feed rates (1.0, 1.5, and 3.0 kg min−1), examining the interaction of parameters for grinding oven-dried Yang-Na leaves. Ethanol extraction initially evaluated the influence of Yang-Na leaf powder with diverse particle sizes. When sorting particle size, the crude extract yield increased as the particle size decreased, with 0.038–0.150 mm particles yielding the highest extraction, although yields decline when the particle size is lower than 0.038 mm. The average particle sizes, production capacity, and fineness modulus all exhibited a significant decrease as the sieve size and feeding rate were reduced, while the specific energy consumption showed an inversely proportional relationship with these parameters. Intriguingly, the crude extract yield remained independent of the average particle size. Notably, the highest yield (14.79 g kg−1) was derived from a 0.31 mm average particle size, ground with a 1.5 mm sieve and a 3 kg min−1 feeding rate. This suggests that the pretreatment, involving both grinding conditions and sorting size, has an impact on the performance of the extraction process. However, this study offers an energy-efficient alternative, advocating for using average particle sizes without prior sorting, streamlining the extraction process while maintaining substantial yields. These insights underline the crucial influence of particle size and grinding techniques, advancing our understanding of efficient herbal extraction techniques for industrial applications.

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Paecilomyces: An Eco-Friendly Approach to Control Agricultural Pests and Pathogens

Chhipa,

Singh,

Deshmukh

2024

Entomopathogenic Fungi

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Antifungal Activity of Bioactive Compounds Produced by the Endophytic Fungus Paecilomyces sp. (JN227071.1) against Rhizoctonia solani

Cited by 12 publications

References 53 publications

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Xenomyrothecium tongaense PTS8: a rare endophyte of Polianthes tuberosa with salient antagonism against multidrug-resistant pathogens

Impact of Grinding and Sorting Particle Size on Phytochemical Yield in Dipterocarpus alatus Leaf Extract

Paecilomyces: An Eco-Friendly Approach to Control Agricultural Pests and Pathogens

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