Antifungal activity of Brevibacillus laterosporus JX-5 and characterization of its antifungal components

Abstract

The establishment of safe and effective methods for controlling fungal disease is an urgent issue in agriculture and forestry. Microbiological control of plant disease is expected to achieve better results than use of chemically derived fungicides. This study aimed to establish Brevibacillus laterosporus JX-5 as a potential microbiological control agent of poplar canker. The bacterium was isolated from the poplar rhizosphere and demonstrated significant growth inhibition of several pathogenic fungi in vitro. The antifungal components of Br. laterosporus JX-5 were isolated and identified. The fermentation broth of Br. laterosporus JX-5 and its main antifungal component, designated as component B, reduced Botryosphaeria dothidea associated canker of the excised poplar branch by 70 and 90%, respectively. Component B is considerably heat-stable, adaptable to a broad pH range, and UV-resistant. It could inhibit Bo. dothidea by permeating the fungal membrane, fracturing the nuclei, damaging the cell wall, and eventually killing the pathogenic fungus. The antifungal activity exhibited by Br. laterosporus JX-5 and its bioactive metabolic products indicate its feasibility as a potential biocontrol agent for plant diseases.

Broad‐spectrum antifungal activity of lipopeptide brevilaterin B and its inhibition effects against Fusarium oxysporum and Penicillium chrysogenum

“…Compared with its MIC in vitro, a much higher concentration of brevilaterin B was needed to prevent fungal infections in vivo. Similar results have been reported for many AFPs, such as bogorol B-JX (Jiang et al, 2015), PgAFP (Delgado et al, 2019) and BP76 (Badosa et al, 2009). Nevertheless, our results suggest that brevilaterin B is promising as a natural antifungal agent for use in the agricultural and food industries.…”

Brevibacillus composti sp. nov., isolated from hyperthermophilic compost

“…Members of this genus are Gram-stain positive, motile, rod-shaped, strictly aerobic or facultatively anaerobic, and produce oval endospores in swollen sporangia [3][4][5][6][7]. Most species of the genus Brevibacillus produce a variety of extracellular products that can inhibit the growth of bacteria [8,9], fungi [10][11][12] and invertebrates [13,14].…”

Streptomyces and Bacillus species utilize volatile organic compounds to impact Fusarium oxysporum f.sp. vasinfectum race 4 (Fov4) virulence and suppress Fusarium wilt in Pima cotton

“…The bacterial isolates collected and investigated in this research, including Bacillus [29,30], Streptomyces [15,16], Paenibacillus [31][32][33], and Brevibacillus [34][35][36] species, are widely acknowledged with their antifungal properties and some are commercially used as biocontrol organisms. Notably, Bacillus species have shown incredible potential as biocontrol agents because of their antimicrobial metabolites and enzymes [12-14, 30, 37].…”