Antifungal activity of carvacrol and antifungal agent combinations against non-albicans Candida species

Nóbrega, Jefferson Rodrigues; Sousa, Paula Mariane Silva; Mota, Kelly Samara de Lira; Cordeiro, Laísa Vilar; Júnior, Francisco Patrício de Andrade; Oliveira, Wylly Araújo de

doi:10.14808/sci.plena.2019.104601

Cited by 5 publications

(6 citation statements)

References 22 publications

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“…Compostos químicos constituintes dos OEs como o eugenol, timol, carvacrol, também são destaques em diversos estudos sobre ação antimicrobiana. Diferentes pesquisas revelam alta atividade fungicida e efeitos sinérgicos entre os compostos químicos no controle de fungos fitopatógenos como A. alternata, C. fructicola, Fusarium verticillioides, Rhizopus stolonifer e outros, como Candida não-albicans, por exemplo (NÓBREGA, et al, 2019;OCHOA-VELASCO, 2016). Assim como, o principal composto químico do OE de canela, cinamaldeído, tem sido utilizado por suas propriedades farmacológicas e biológicas, com poderosa atividade contra fungos e outros microrganismos (FIGUEIREDO et al, 2017).…”

Section: Discussionunclassified

ATIVIDADE ANTIFÚNGICA DE ÓLEOS ESSENCIAIS FRENTE A Macrophomina phaseolina

Figueiredo

Silva

Morais

2023

NAT

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Macrophomina phaseolina é um fitopatógeno de solo com ampla distribuição geográfica, responsável por ocasionar perdas econômicas graves incitando sintomas de podridões no sistema radicular e caule das plantas infectadas. Há uma demanda mundial crescente por novos tratamentos, que promovam a segurança alimentar e nutricional, com substâncias mais eficazes e menos danosas ao equilíbrio dos ecossistemas. O objetivo da pesquisa foi avaliar o efeito antifúngico in vitro de óleos essenciais (OEs) sobre o crescimento micelial de M. phaseolina. Os OEs utilizados foram testados nas doses 0,8; 1,6; 3,2; 6,4 e 12,8 μL por superfície do meio de cultura BDA (batata-dextrose-ágar). Foram analisadas a inibição do crescimento micelial e a taxa de crescimento micelial. As avaliações consistiram em aferições dos diâmetros de crescimento com 3 e 6 dias de incubação. Os OEs de canela, a partir da dose inicial testada; orégano e cravo, na dose 3,2 μL demonstraram atividade antifúngica contra M. phaseolina. Já os OEs de manjericão, melaleuca, citronela, menta e tomilho demonstraram atividade fungistática. Os OEs de gengibre e laranja inibiram menos de 40% o crescimento micelial. Atingiu-se a inibição total do crescimento micelial com os OEs de canela, orégano e cravo, portanto, apresentaram potencial de uso como agente biofungicida. Palavras-chave: produtos naturais, podridão radicular, química verde. Antifungal activity of essential oils against Macrophomina phaseolina ABSTRACT: Macrophomina phaseolina is a soil‐borne phytopathogen with has a wide geographical distribution, being responsible for causing serious economic losses inciting stem and root rot symptoms of infected plants. There is a growing worldwide demand for new treatments that promote food and nutritional security, with substances that are more effective and less harmful to the balance of ecosystems. Thus, this research aimed to evaluate the in vitro antifungal effect of essential oils (EOs) on the mycelial growth of M. phaseolina. The EOs used were tested at doses 0.8; 1.6; 3.2; 6.4 and 12.8 μL per surface of the PDA (potato-dextrose-agar) culture medium. Mycelial growth inhibition and mycelial growth rate were analyzed. The evaluations consisted of measurements of the growth diameters with 3 and 6 days of incubation. The cinnamon EO, from the initial tested concentration; oregano and cloves EOs, at a concentration of 3.2 μL demonstrated antifungal activity against M. phaseolina. The EOs of basil, tea tree, citronella, mint and thyme showed a fungistatic activity. Ginger and sweet Orange EOs inhibited less than 40% of mycelial growth. The complete inhibition of mycelial growth was achieved with cinnamon, oregano and clove EOs, therefore, they presented potential for use as a biofungicide agent. Keywords: natural products; root rot; green chemistry.

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Section: Discussionunclassified

ATIVIDADE ANTIFÚNGICA DE ÓLEOS ESSENCIAIS FRENTE A Macrophomina phaseolina

Figueiredo

Silva

Morais

2023

NAT

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“…Antimicrobial combinations can result in synergism, antagonism, indifference, or additivity, and using the checkerboard method it is possible to evaluate these interactions against different pathogens [42][43][44].…”

Section: Discussionmentioning

confidence: 99%

“…The association between natural products and antifungal agents is employed as a feasible approach to bypass the limitations of antifungal monotherapy [44][45][46], seeking to broaden the spectrum of therapeutic efficacy, prevent the development of antifungal resistance, and reduce toxicity. Thus, this strategy seeks to achieve greater success in treatment, which has already been observed in combined therapies of certain antifungals [46][47][48].…”

Section: Discussionmentioning

confidence: 99%

(R)-(+)-β-Citronellol and (S)-(−)-β-Citronellol in Combination with Amphotericin B against Candida Spp.

Silva

Neto

Cordeiro

et al. 2020

IJMS

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The enantiomers (R)-(+)-β-citronellol and (S)-(−)-β-citronellol are present in many medicinal plants, but little is understood about their bioactivity against Candida yeasts. This study aimed to evaluate the behavior of positive and negative enantiomers of β-citronellol on strains of Candida albicans and C. tropicalis involved in candidemia. The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined. The evaluation of growth kinetics, mechanism of action, and association studies with Amphotericin B (AB) using the checkerboard method was also performed. R-(+)-β-citronellol and S-(−)-β-citronellol presented a MIC50% of 64 µg/mL and a MFC50% of 256 µg/mL for C. albicans strains. For C. tropicalis, the isomers exhibited a MIC50% of 256 µg/mL and a MFC50% of 1024 µg/mL. In the mechanism of action assay, both substances displayed an effect on the fungal membrane but not on the fungal cell wall. Synergism and indifference were observed in the association of R-(+)-β-citronellol and AB, while the association between S-(−)-β-citronellol and AB displayed synergism, additivity, and indifference. In conclusion, both isomers of β-citronellol presented a similar profile of antifungal activity. Hence, they can be contemplated in the development of new antifungal drugs providing that further research is conducted about their pharmacology and toxicity.

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“…No antagonist effect was observed. A synergistic effect was observed with the combination of carvacrol/ketoconazole and Amphotericin B against C. guilliermondii LM-103 and carvacrol/fluconazole against C. parapsilosis ATCC 22019 [39].…”

Section: Antimicrobial Activity Of Carvacrolmentioning

confidence: 94%

Carvacrol—A Natural Phenolic Compound with Antimicrobial Properties

et al. 2023

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The main purpose of this article is to present the latest research related to selected biological properties of carvacrol, such as antimicrobial, anti-inflammatory, and antioxidant activity. As a monoterpenoid phenol, carvacrol is a component of many essential oils and is usually found in plants together with its isomer, thymol. Carvacrol, either alone or in combination with other compounds, has a strong antimicrobial effect on many different strains of bacteria and fungi that are dangerous to humans or can cause significant losses in the economy. Carvacrol also exerts strong anti-inflammatory properties by preventing the peroxidation of polyunsaturated fatty acids by inducing SOD, GPx, GR, and CAT, as well as reducing the level of pro-inflammatory cytokines in the body. It also affects the body’s immune response generated by LPS. Carvacrol is considered a safe compound despite the limited amount of data on its metabolism in humans. This review also discusses the biotransformations of carvacrol, because the knowledge of the possible degradation pathways of this compound may help to minimize the risk of environmental contamination with phenolic compounds.

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Antifungal activity of carvacrol and antifungal agent combinations against non-albicans Candida species

Cited by 5 publications

References 22 publications

ATIVIDADE ANTIFÚNGICA DE ÓLEOS ESSENCIAIS FRENTE A Macrophomina phaseolina

ATIVIDADE ANTIFÚNGICA DE ÓLEOS ESSENCIAIS FRENTE A Macrophomina phaseolina

(R)-(+)-β-Citronellol and (S)-(−)-β-Citronellol in Combination with Amphotericin B against Candida Spp.

Carvacrol—A Natural Phenolic Compound with Antimicrobial Properties

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