Antifungal Activity of Centella asiatica on Radial Growth of Aspergillus species

Abstract

The study was conducted to observe the antifungal activity of Centella asiatica against Aspergillus sp. Samples were collected from the local area of the Tangail district in Bangladesh. The bread was taken for isolating Aspergillus sp. in addition, Potato dextrose agar and malt extract agar were used as a media for culture and pure culture. One control group and two sample group (1%, 5%) hydrophilic extract of C. asiatica has been used. The routine observation was done for a total of 5 days after 24 hours. After routine observation, it has noticed that colony diameter and number of colonies increased rapidly from day 1 to day 5. The fungal growth was null of 5% solution both of PDA, MEA media at the first day and these increased with rising time on 5th days there were 1900 and 1600 respectively for black species and 1500 and 2500 respectively for green sp. Whereas compared with control group the growth was measured 1000, 1200 respectively at day 1 and these increased with rising time these numbers were 4000, 4200 respectively at day 5 for black. On the other hand, at 1st day the growth was measured 1100, 1200 respectively and these increased with rising time on 5th days there were 4200, 4300 respectively for green sp. However, at the end of the study, a remarkable change was observed between the control group and sample group after 1st, 2nd, 3rd, 4th and 5th days. Finally, the study showed that the bioactive compounds of C. asiatica are a potential source of preservative that inhibits the growth of fungus.