Antifungal Activity of Chitinases from Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9

Nguyen, Nam Van; Kim, Young‐Ju; Oh, Kyung-Taek; Jung, Woo‐Jin; Park, Ro-Dong

doi:10.1007/s00284-007-9033-4

Cited by 25 publications

(13 citation statements)

References 15 publications

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“…The antifungal activity exhibited a close relationship with production of chitinase and siderophores (Cazorla et al , ; Pliego et al , ). Chitinolytic enzymes have been studied as potential antifungal agents against chitin‐bearing plant pathogens because the enzymes play a key role in the mechanism of parasitic entry into host cells (Nguyen et al , ). Siderophores bind to the available form of iron (Fe 3+ ) in the rhizosphere, thus making it unavailable to the phytopathogens and protecting the plant health (Ramos‐Solano et al , ).…”

Section: Discussionsupporting

confidence: 90%

Phosphate solubilisation and plant growth promoting potential by stress tolerant Bacillus sp. isolated from rhizosphere of apple orchards in trans Himalayan region of Himachal Pradesh

Mehta

Walia

Chauhan

et al. 2013

Annals of Applied Biology

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Phosphate-solubilising ability and co-production of plant growth promoting traits of stress tolerant Bacillus subtilis CB 8 A isolated from apple rhizosphere was tested under in vitro conditions against a wide range of temperature (30-45°C), pH (7-9) and salt (0-5%) stresses. Under the extremes of temperature (45°C), pH-9 and salt concentration (5%), production of soluble phosphate, indole acetic acid, siderophore and antifungal activity against Dematophora necatrix were reduced by 71.09%, 75.29%, 90.3% and 88.47%, respectively. Per cent decrease in P-solubilisation at extreme temperature (45°C) and normal pH (7) without salt concentration was 36.23%; at extreme pH (9) and normal temperature (37°C) without salt concentration was 23.45% and at extreme salt concentration (5%), optimum temperature (37°C) and pH (7) was 36.7%. P-solubilisation by CB 8 A was inversely correlated with pH (r = −0.78) and positively correlated with siderophore production (r = 0.81), indole acetic acid (r = 0.58) and antifungal activity (r = 0.63). Gluconic acid (1.43%) and citric acid (0.67%) were detected as major organic acids. P-solubilisation and nitrogen fixing abilities of B. subtilis CB 8 A were confirmed by amplification of gdh and nif H genes. The ability of CB8A showing plant growth promoting rhizobacteria (PGPR) traits at a wide range of temperature, pH and varying salt concentration can be exploited for developing multifunctional biofertiliser in apple orchards.

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Section: Discussionsupporting

confidence: 90%

Phosphate solubilisation and plant growth promoting potential by stress tolerant Bacillus sp. isolated from rhizosphere of apple orchards in trans Himalayan region of Himachal Pradesh

Mehta

Walia

Chauhan

et al. 2013

Annals of Applied Biology

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“…This low value may be due to synergistic action of different isoforms present in the crude supernatant and the loss of chitinase activity during the purification process. Those chitinases had a size range of 40-88 kDa which was typically larger than the sizes of plant (25-40 kDa) and bacterial (20-60 kDa) chitinases [13,16,17]. It has been also reported that the optimum temperature of insect chitinases ranged from 20 to 60°C toward different substrates such as colloidal chitin, GlcNAc 5 and glycol chitin [5,10,16].…”

Section: Discussionmentioning

confidence: 96%

“…Culture supernatant of Bacillus thuringiensis (Bt; strain BUPM255) was reported to have high antifungal activity against Aspergillus niger [11]. Further, the chitinases from two fungal strains can degrade the cell walls of Fusarium solani and also inhibit microconidial germination in this species [17]. Chitinases are also potentially useful as food preservatives.…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Chitinases from Silkworm (Bombyx mori) and Bollworm (Helicoverpa armigera)

Zhang

Liu

Tian

et al. 2011

Cell Biochem Biophys

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Chitinases play an important role in the degradation of the cuticular chitin during the process of ecdysis. In this study, we compared the chitinases of two insect species, Bombyx mori (silkworm) and Helicoverpa armigera (bollworm), to assess the relation between characteristics and chitinase patterns. Differences between two chitinases were observed after purification using ammonium sulfate precipitation, affinity chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay. Although the specific activities of the purified enzymes were different, the purification yields were similar. One band of 88 kDa was observed for B. mori, and the other band of 75 kDa was detected for H. armigera. When a range of properties was tested, it was found that the optimum temperatures of B. mori and H. armigera chitinases were 45 and 50°C, respectively; the optimum pH value was 6.0 for both chitinases. Mn(2+) played catalytic role while Cu(2+) and SDS strongly inhibited activities of both enzymes. Between two chitinases, differences in K (M) were also observed. K (M) of chitinase from silkworm and bollworm was found to be 22.3 and 41.0 μmol/l, respectively. Both the chitinases significantly inhibited the spore germination of two fungal species, Saccharomyces cerevisiae and Penicillium.

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“…Chitin is the major structural component of various organisms, including fungi, insects, and crustaceans (Chernin et al, 1998). Chitinase is an enzyme that functions as a protective component of some bacteria in fighting against fungal or other pathogens with chitinous exoskeleton (Neiendam Nielsen & Sorensen, 1999;Nguyen et al, 2008). In C. violaceum, chitinase production was regulated by QS and upregulated by antibiotics, indicating that the antibiotics at SICs contribute to the success of C. violaceum in its competition with other inhabitants by improving QS-regulated behaviors.…”

Section: Discussionmentioning

confidence: 99%

Antibiotics at subinhibitory concentrations improve the quorum sensing behavior ofChromobacterium violaceum

Liu

Wang

Zhu

et al. 2013

FEMS Microbiol Lett

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Increasing evidence has shown that antibiotics function as intermicrobial signaling molecules instead of killing weapons. However, mechanisms and key factors that are involved in such functions remain poorly understood. Earlier findings have associated antibiotic signaling with quorum sensing (QS); however, results varied among experiments, antibiotics, and bacterial strains. In this study, we found that antibiotics at subinhibitory concentrations improved the violacein-producing ability of Chromobacterium violaceum ATCC 12472. Quantitative real-time polymerase chain reaction of QS-associated gene transcripts and bioassay of violacein production in a QS mutant strain demonstrated that antibiotics enhanced the production of N-acyl-L-homoserine lactones (AHLs; QS signaling molecules) and increased AHL-inducing QS-mediated virulence, including chitinase production and biofilm formation. Moreover, a positive flagellar activity and an increased bacterial clustering ability were found, which are related to the antibiotic-induced biofilm formation. Our findings suggested that antibiotic-mediated interspecific signaling also occurs in C. violaceum, thereby expanding the knowledge and language of cell-to-cell communication.

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Antifungal Activity of Chitinases from Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9

Cited by 25 publications

References 15 publications

Phosphate solubilisation and plant growth promoting potential by stress tolerant Bacillus sp. isolated from rhizosphere of apple orchards in trans Himalayan region of Himachal Pradesh

Phosphate solubilisation and plant growth promoting potential by stress tolerant Bacillus sp. isolated from rhizosphere of apple orchards in trans Himalayan region of Himachal Pradesh

Comparative Characterization of Chitinases from Silkworm (Bombyx mori) and Bollworm (Helicoverpa armigera)

Antibiotics at subinhibitory concentrations improve the quorum sensing behavior ofChromobacterium violaceum

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