Antifungal activity of hop extracts and compounds against the wheat pathogen Zymoseptoria tritici

Bocquet, Laetitia; Rivière, Céline; Dermont, Charles; Samaillie, Jennifer; Hilbert, Jean‐Louis; Halama, Patrice; Siah, Ali; Şahpaz, Sevser

doi:10.1016/j.indcrop.2018.05.061

Cited by 69 publications

(63 citation statements)

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“…Currently, the research on alternative natural products with potential use in pest management 49 strategies is very active (Bocquet et al, 2018; Eloff et al, 2017; Wu et al, 2018). To this purpose, 50 phenolic-rich plant extracts were shown to display antifungal activity against different pathogenic 51 fungi, including Fusarium oxysporum, Rhizoctonia solani, Phytophthora nicotianae, Alternaria 52 alternata and Aspergillus species (Eloff et al, 2017; de Rodríguez et al, 2015; Wu et al, 2018).…”

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confidence: 99%

Phenolic compounds profile of water and ethanol extracts of Euphorbia hirta L. leaves showing antioxidant and antifungal properties

Mekam

Martini

Julienne

et al. 2019

South African Journal of Botany

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1The bioactive chemical constituents of water and ethanol extracts of Euphorbia hirta L. leaves have 2 been identified and quantified using an un-targeted mass spectrometric approach. The study allowed 3 the tentative identification of 123 individual phenolic compounds and 18 non-phenolic 4 phytochemicals, most of them described in Euphorbia hirta L. leaves for the first time. 5 Gallotannins, hydroxybenzoic and hydroxycinnamic acids were the most abundant phenolic classes 6 in Euphorbia hirta L. leaves, representing together the 71. 5% (26.3%, 25.2% and 20%, 7 respectively) of the total amount of identified phenolics. The main phenolic compounds detected 8 were tri-O-galloyl-glucose isomers, feruloyl-coniferin, tetra-O-galloyl-glucose isomers, di-O-9 galloyl-glucose isomers, ethyl-gallic acid, protocatechuic acid-O-pentoside-O-hexoside, 5-O-10 caffeoyl-quinic acid trans isomer and digalloyl-quinic acid. Feruloyl-coniferin was found to be 11 approximately six times more concentrated in the ethanol extract with respect to the water extract. 12 The ethanol extract exhibited higher ABTS (1338.3 ± 85.3 and 802.3 ± 91.0 μmol ascorbic acid 13 equivalent/gram of dry extract, respectively) and superoxide anion (2014.6 ± 78.6 and 1528.0 ± 14 111.7 μmol ascorbic acid equivalent/gram of dry extract, respectively) scavenging abilities than the 15 water extract. Additionally, the ethanol extract also showed a remarkable anti-fungal effect against 16 Fusarium oxysporum f. sp. vasinfectum, Alternaria solani and Rhizoctonia solani. This study 17 provides new information about Euphorbia hirta L., offering reasons to promote this plant species 18 as rich source of phenolics and an excellent source of antifungal molecules that might have a 19 prospective use in controlling fungal diseases of vegetable crops. 20 Euphorbia hirta L. (E. hirta) is a plant species commonly found in all tropical countries worldwide, 23 including Cameroon. E. hirta belongs to the spurge family of Euphorbiaceae. Although it can be 24 seen lying down sometimes, it is usually upright, slender-stemmed, spreading up to 80 cm tall (Abu 25 et al., 2011). 26E. hirta is a very popular medicinal herb and has been used since ancient times as decoction or 27 infusion in traditional remedies to treat gastro-intestinal diseases and disorders (e.g. intestinal 28 parasites, diarrhoea and peptic ulcer), skin problems and asthma (Huang et al., 2012). More 29 recently, extracts from E. hirta have shown a broad range of biological properties, including 30 antimicrobial, antifungal, anti-inflammatory, antioxidant, anticancer and antidiabetic activities 31 (Almosnid et al., 2018;Kumar et al., 2010;Li et al., 2015). Several phytochemicals have been 32 already extracted and identified from E. hirta leaves, such as terpenoids, coumarins, lignans and 33 phenolic compounds (Kumar et al., 2010;Li et al., 2015;Yi et al., 2012). The latter components, 34 widely known for their antioxidant and biological activities, have been rarely investigated. In this 3...

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confidence: 99%

Phenolic compounds profile of water and ethanol extracts of Euphorbia hirta L. leaves showing antioxidant and antifungal properties

Mekam

Martini

Julienne

et al. 2019

South African Journal of Botany

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“…In addition, numerous antimicrobial activities of hops essential oils and crude leaf extracts are described (reviewed in [ 48 , 51 ]), including antibacterial effects towards Gram-positive and Gram-negative bacteria, inhibition of replication and proliferation of certain viruses, and potent activity of some prenylated chalcone derivatives against malaria-causing Plasmodium falciparum . Hops phenolics also display antifungal activity against Trichophyton mentagrophytes and T. rubrum , weak inhibition of Fusarium oxysporum and Zymoseptoria tritici , and very low activity against Candida albicans , C. glabarta , and some Pencillium and Aspergillus species [ 48 , 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, only a few studies have reported on phenolics from hop leaves, stems, and roots [ 52 , 53 , 54 , 55 ], especially in relation to their antifungal activity [ 52 ]. Here, we quantified the total polyphenols in roots and stems of 14 hop varieties before flowering and at the mature hops stage.…”

Section: Discussionmentioning

confidence: 99%

“…Compared to hops essential oil (IC 50 = 0.36 g/L; 100% inhibition) or crude hops extracts (IC 50 = 0.73 g/L; 85% inhibition), the inhibitory activity of methanol extracts from hop leaves, stems, and roots was relatively low (25–35% inhibition) when assessed against the wheat pathogen Zymoseptoria tritici [ 52 ]. In our study, dose response curves of methanol extracts from hop roots and stems revealed that the total polyphenols from stems (IC 50 of 130–211 mg/L) were ten times more active against V. nonalfalfae than those from roots (IC 50 of 1171–1804 mg/L).…”

Section: Discussionmentioning

confidence: 99%

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Hop Polyphenols in Relation to Verticillium Wilt Resistance and Their Antifungal Activity

Berne

Kovačević

Kastelec

et al. 2020

Plants

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(1) Background: Verticillium wilt (VW) of hop is a devastating disease caused by the soil-borne fungi Verticillium nonalfalfae and Verticillium dahliae. As suggested by quantitative trait locus (QTL) mapping and RNA-Seq analyses, the underlying molecular mechanisms of resistance in hop are complex, consisting of preformed and induced defense responses, including the synthesis of various phenolic compounds. (2) Methods: We determined the total polyphenolic content at two phenological stages in roots and stems of 14 hop varieties differing in VW resistance, examined the changes in the total polyphenols of VW resistant variety Wye Target (WT) and susceptible Celeia (CE) on infection with V. nonalfalfae, and assessed the antifungal activity of six commercial phenolic compounds and total polyphenolic extracts from roots and stems of VW resistant WT and susceptible CE on the growth of two different V. nonalfalfae hop pathotypes. (3) Results: Generally, total polyphenols were higher in roots than stems and increased with maturation of the hop. Before flowering, the majority of VW resistant varieties had a significantly higher content of total polyphenols in stems than susceptible varieties. At the symptomatic stage of VW disease, total polyphenols decreased in VW resistant WT and susceptible CE plants in both roots and stems. The antifungal activity of total polyphenolic extracts against V. nonalfalfae was higher in hop extracts from stems than those from roots. Among the tested phenolic compounds, only p-coumaric acid and tyrosol markedly restricted fungal growth. (4) Conclusions: Although the correlation between VW resistance and total polyphenols content is not straightforward, higher levels of total polyphenols in the stems of the majority of VW resistant hop varieties at early phenological stages probably contribute to fast and efficient activation of signaling pathways, leading to successful defense against V. nonalfalfae infection.

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“…Moreover, besides medical purposes (see for other refs [ 10 , 11 ]), the interest in this plant is increasing in the light of its possible use also in pest control. Recent papers, in fact, highlighted activities of hop against detrimental fungi [ 12 ], mites [ 13 ], and insects [ 14 , 15 ]. This aspect appears extremely urgent since, as known, the traditional control of stored-product insect pests has relied primarily on synthetic insecticides like organochlorines, organophosphates, methyl bromide, and phosphine [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of Humulus lupulus (L.) Essential Oil and Its Main Components against Sitophilus granarius (L.)

et al. 2020

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Besides its use in the brewing industry, hop cones appear as a powerful source of biologically active compounds, already checked for their putative anticancer, antimicrobial, and other bioactivities. Conversely, hop use in pest control remains to date under-investigated. Therefore, the biological activity of hop essential oil (EO) and its main constituents was investigated here against Sitophilus granarius. Adult contact toxicity was found 24 h after treatment with hop EO (LD50/LD90 13.30/40.23 µg/adult), and its three most abundant components, α-humulene, β-myrcene, and β-caryophyllene (LD50/LD90 41.87/73.51, 75.91/126.05, and 138.51/241.27 µg/adult, respectively); negligible variations at 48 h, except for α-humulene (LD50/LD90 26.83/49.49 µg/adult), were found. The fumigant toxicity of the EO and terpenes was also checked: in the absence of wheat grains, β-myrcene showed the highest inhalation toxicity (LC50/LC90 72.78/116.92 mg/L air), whereas α-humulene, β-caryophyllene, and the EO induced similar values (LC50/LC90 about 130/200 mg/L air); with the exception for EO, the wheat presence increased (30–50%) LC50/LC90 values. Moreover, EO and terpenes were perceived by insect antennae and elicited repellent activity. Only β-caryophyllene showed an anticholinesterase effect, this suggesting that different mechanisms of action should be responsible for hop EO toxicity. Therefore, hop EO appears suitable for developing control means against this pest.

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Antifungal activity of hop extracts and compounds against the wheat pathogen Zymoseptoria tritici

Cited by 69 publications

References 32 publications

Phenolic compounds profile of water and ethanol extracts of Euphorbia hirta L. leaves showing antioxidant and antifungal properties

Phenolic compounds profile of water and ethanol extracts of Euphorbia hirta L. leaves showing antioxidant and antifungal properties

Hop Polyphenols in Relation to Verticillium Wilt Resistance and Their Antifungal Activity

Biological Activity of Humulus lupulus (L.) Essential Oil and Its Main Components against Sitophilus granarius (L.)

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