Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates

Mijatovic, Stefan; Antić-Stanković, Jelena; Čalovski, Ivana Čolović; Dubljanin, Eleonora; Pljevljakušić, Dejan; Bigović, Dubravka; Džamić, Aleksandar

doi:10.3390/molecules27196300

Cited by 15 publications

(6 citation statements)

References 38 publications

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“…3). However, the observed MLC values were higher than the MLC values of other medicinal plants reported in literature [25][26][27][28].The authors did not come across…”

Section: Antimicrobial Activitymentioning

confidence: 54%

Assessment of the phytochemical composition and antimicrobial properties of Tapinanthus bangwensis leaves hosted by the branches of Persea americana

Sakyiamah

Gordon²,

Bolah³

et al. 2023

BMC Complement Med Ther

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Background Medicinal plants represent a valuable source for new effective and safe antimicrobial drugs making them an alternative therapy. Existing antimicrobial agents are costly and mostly associated with possible side effects. The aim of the present study therefore, was to assess the antimicrobial property and phytochemical composition of hydroethanolic extract of Tapinanthus bangwensis leaves and its fractions. Method T. bangwensis leaves (harvested from its host plant, Persea americana) was extracted by cold maceration with 70% ethanol and further fractionated with different organic solvents using the solvent partitioning method to obtain the crude extract, petroleum ether, chloroform, ethyl acetate and the resulting aqueous fractions. The phytochemical constituents of the extracts were screened and quantified. Also, the TLC of the extracts were analyzed to serve as a fingerprint. Using the agar diffusion and broth dilution methods, the antimicrobial properties of the extracts were assessed. Results The study showed that the hydroethanolic (70%) crude extract of T. bangwensis leaves and its fractions contain phenolic compounds, flavonoids, saponins, phytosterols and reducing sugars. The phytoconstituents were well extracted into the ethyl acetate fraction than the other fractions evidenced in the high levels (p < 0.0001) of saponins (66.47 ± 1.72% w/w), phenolic compounds (77.75 ± 1.06 mg/100 mg GAE) and flavonoids (44.34 ± 0.06 mg/100 mg QE) contents. From the antimicrobial studies, all the microorganisms tested exhibited varying degrees of susceptibility to the extracts with MIC values between 0.78 to 12.5 mg/mL. The crude extract of T. bangwensis leaves, its ethyl acetate and chloroform fractions also exhibited lethal antimicrobial activity with MLC between 6.25 to 50 mg/mL. Conclusion The crude extract of T. bangwensis leaves and its fractions demonstrated antimicrobial properties against Escherichia coli, Staphylococcus aureus, Staphylococcus saprophyticus and Candida albicans, thereby representing a potential source of natural antimicrobial agent. Further study is required to identify and isolate antimicrobial compounds from the plant for the development of the natural bioactive antimicrobial agents.

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“…3). However, the observed MLC values were higher than the MLC values of other medicinal plants reported in literature [25][26][27][28].The authors did not come across…”

Section: Antimicrobial Activitymentioning

confidence: 54%

Assessment of the phytochemical composition and antimicrobial properties of Tapinanthus bangwensis leaves hosted by the branches of Persea americana

Sakyiamah

Gordon²,

Bolah³

et al. 2023

BMC Complement Med Ther

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“…Both fluconazole susceptible and fluconazole resistant isolates, as well as wild-type (WT) and non-WT caspofungin isolates, respond to the same concentration. Despite the fact that systemic antifungal medications are the foundation of the preventative treatment of invasive candidiasis, further research is required to judge the efficacy of using essential oils as aromatherapy with highrisk patients [23].…”

Section: Antifungal Activitymentioning

confidence: 99%

Khuzāma (Lavandula angustifolia Mill.): Pharmacological Action and Therapeutic Uses in Perspective of Unani Medicine: A Review

Kalam,

Habib,

Arzoo

et al. 2024

JOCAMR

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Lavandula angustifolia Mill., commonly known as lavender, is a highly regarded medicinal herb in Unani Medicine. Its aerial parts, including leaves and flowers, are utilized for their therapeutic properties, and the plant is a common ingredient in cosmetic products. The essential oil derived from lavender is known for its sedative, carminative, anti-depressive, anti-inflammatory, and antimicrobial properties. These pharmacological effects are attributed to various bioactive compounds like betulin, betulinic acid, lactone, 3 beta-formyl ursolic acid, essential oil, glycolic acid, valeric acid, and linalyl acetate. This review aims to comprehensively explore Lavandula angustifolia Mill., covering identification, pharmacological actions, traditional uses, and recent scientific investigations. Utilizing a diverse range of sources, including scientific literature and research studies, the review adopts a comprehensive approach to compile information. The diverse array of bioactive constituents in lavender contributes to its medicinal profile, making it a versatile herbal remedy. Emphasis is placed on documenting and highlighting various pharmacological activities, reinforcing lavender's status as a potent therapeutic agent. Beyond its medicinal applications, the review delves into lavender's historical uses in Unani Medicine and its contemporary role in cosmetic formulations. Lavandula angustifolia Mill. emerges as a herbal powerhouse, offering a holistic approach to health and well-being. This review serves as a valuable resource for practitioners of traditional medicine and modern science alike, bridging the gap between historical wisdom and contemporary research in the realm of herbal medicine.

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“…In accordance with previous research [35,36], a single larger colony of C. albicans cells in the exponential growth phase was picked from the medium. After washing with PBS three times, a standard turbidimeter was used to adjust the cell suspension to 15 × 10 4 CFU/ml, which was then immediately diluted with RPMI 1640 to 5 × 10 4 CFU/ml.…”

Section: Time-kill Curve Testmentioning

confidence: 99%

Mechanism and bioinformatics analysis of the effect of berberine-enhanced fluconazole against drug-resistant Candida albicans

Wu,

Jia,

et al. 2024

BMC Microbiol

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Biofilms produced by Candida albicans present a challenge in treatment with antifungal drug. Enhancing the sensitivity to fluconazole (FLC) is a reasonable method for treating FLC-resistant species. Moreover, several lines of evidence have demonstrated that berberine (BBR) can have antimicrobial effects. The aim of this study was to clarify the underlying mechanism of these effects. We conducted a comparative study of the inhibition of FLC-resistant strain growth by FLC treatment alone, BBR treatment alone, and the synergistic effect of combined FLC and BBR treatment. Twenty-four isolated strains showed distinct biofilm formation capabilities. The antifungal effect of combined FLC and BBR treatment in terms of the growth and biofilm formation of Candida albicans species was determined via checkerboard, time-kill, and fluorescence microscopy assays. The synergistic effect of BBR and FLC downregulated the expression of the efflux pump genes CDR1 and MDR, the hyphal gene HWP1, and the adhesion gene ALS3; however, the gene expression of the transcriptional repressor TUP1 was upregulated following treatment with this drug combination. Furthermore, the addition of BBR led to a marked reduction in cell surface hydrophobicity. To identify resistance-related genes and virulence factors through genome-wide sequencing analysis, we investigated the inhibition of related resistance gene expression by the combination of BBR and FLC, as well as the associated signaling pathways and metabolic pathways. The KEGG metabolic map showed that the metabolic genes in this strain are mainly involved in amino acid and carbon metabolism. The metabolic pathway map showed that several ergosterol (ERG) genes were involved in the synthesis of cell membrane sterols, which may be related to drug resistance. In this study, BBR + FLC combination treatment upregulated the expression of the ERG1, ERG3, ERG4, ERG5, ERG24, and ERG25 genes and downregulated the expression of the ERG6 and ERG9 genes compared with fluconazole treatment alone (p < 0.05).

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Antifungal Activity of Lavandula angustifolia Essential Oil against Candida albicans: Time-Kill Study on Pediatric Sputum Isolates

Cited by 15 publications

References 38 publications

Assessment of the phytochemical composition and antimicrobial properties of Tapinanthus bangwensis leaves hosted by the branches of Persea americana

Assessment of the phytochemical composition and antimicrobial properties of Tapinanthus bangwensis leaves hosted by the branches of Persea americana

Khuzāma (Lavandula angustifolia Mill.): Pharmacological Action and Therapeutic Uses in Perspective of Unani Medicine: A Review

Mechanism and bioinformatics analysis of the effect of berberine-enhanced fluconazole against drug-resistant Candida albicans

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