Antifungal Activity of Partially Purified Bacterial Chitinase Against Alternaria alternata

Dikbaş, Neslihan; Uçar, Sevda; Tozlu, Elif; Kotan, Merve Şenol; Kotan, Recep

doi:10.1007/s10341-022-00716-4

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…The analysis of enzymes exhibiting a variety of antifungal activities indicated that most of them were hydrolases acting on polysaccharides present in the structure of the fungal cell wall or involved in the formation of biofilms. The greatest effect was observed in the case of chitinases [67][68][69][70][71][72][73][74][75][76][77][78], among which there were both exo-and endochitinases. At the same time, their simultaneous presence in the enzyme complexes used to suppress the growth of fungi was the most successful [77,79].…”

Section: Antifungal Enzymes Using Cell Structural Components Of Fungi...mentioning

confidence: 97%

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Lyagin

Aslanli

Domnin

et al. 2023

IJMS

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Active research of metal-containing compounds and enzymes as effective antifungal agents is currently being conducted due to the growing antifungal resistance problem. Metals are attracting special attention due to the wide variety of ligands that can be used for them, including chemically synthesized and naturally obtained variants as a result of the so-called “green synthesis”. The main mechanism of the antifungal action of metals is the triggering of the generation and accumulation of reactive oxygen species (ROS). Further action of ROS on various biomolecules is nonspecific. Various hydrolytic enzymes (glucanases and proteases), in turn, exhibit antifungal properties by affecting the structural elements of fungal cells (cell walls, membranes), fungal quorum sensing molecules, fungal own protective agents (mycotoxins and antibiotics), and proteins responsible for the adhesion and formation of stable, highly concentrated populations in the form of biofilms. A wide substrate range of enzymes allows the use of various mechanisms of their antifungal actions. In this review, we discuss the prospects of combining two different types of antifungal agents (metals and enzymes) against mycelial fungi and yeast cells. Special attention is paid to the possible influence of metals on the activity of the enzymes and the possible effects of proteins on the antifungal activity of metal-containing compounds.

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Section: Antifungal Enzymes Using Cell Structural Components Of Fungi...mentioning

confidence: 97%

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Lyagin

Aslanli

Domnin

et al. 2023

IJMS

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“…Chitinases hydrolyze chitin polymers into smaller oligosaccharides or monomers, playing a crucial role in various biological processes, including nutrient recycling, plant defense, and pathogen inhibition (Ezeilo et Bacteria are known to produce chitinases with antifungal properties, which have potential applications in agriculture, medicine, and industry. Among these bacteria, Bacillus subtilis and Serratia marcescens have been extensively studied for their ability to produce chitinases with antifungal activity against a range of fungal pathogens (Jendri Mamangkey et al 2022;Dikbaş et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Investigating the Antifungal Potential of Genetically Modified Hybrid Chitinase Enzymes Derived from Bacillus subtilis and Serratia marcescens

Bouqellah,

Jamil,

Faraag

2024

Preprint

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Chitinases are glycosyl hydrolase enzymes that break down chitin, an integral component of fungal cell walls. Bacteria such as Bacillus subtilis and Serratia marcescens produce chitinases with antifungal properties. In this study, we aimed to generate hybrid chitinase enzymes with enhanced antifungal activity by combining functional domains from native chitinases produced by B. subtilis and S. marcescens. Chitinase genes were cloned from both bacteria and fused together using overlap extension PCR. The hybrid constructs were expressed in E. coli and the recombinant enzymes purified. Gel electrophoresis and computational analysis confirmed the molecular weights and isoelectric points of the hybrid chitinases were intermediate between the parental enzymes. Antifungal assays demonstrated that the hybrid chitinases inhibited growth of the fungus Fusarium oxysporumsignificantly more than the native enzymes. Our results indicate domain shuffling of bacterial chitinases is a promising approach to engineer novel antifungal proteins. This study provides insight into structure-function relationships of chitinases and strategies for generating biotherapeutics with enhanced bioactive properties.

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Fermentation of waste water from agar processing with Bacillus subtilis by metabolomic analysis

Wu,

Duan,

Lin

et al. 2024

Appl Microbiol Biotechnol

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Antifungal Activity of Partially Purified Bacterial Chitinase Against Alternaria alternata

Cited by 5 publications

References 23 publications

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Investigating the Antifungal Potential of Genetically Modified Hybrid Chitinase Enzymes Derived from Bacillus subtilis and Serratia marcescens

Fermentation of waste water from agar processing with Bacillus subtilis by metabolomic analysis

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