The antifungal and antimycotoxigenic activities of the essential oils (EO) from Cuminum cyminum and Laurus nobilis, and their respective principal compounds, cuminaldehyde and 1,8-cineole, were evaluated against fungi of the genus Aspergillus: A.carbonarius, A.niger, A.ochraceus and A.westerdijkiae. The antifungal activity was determined by the contact method and the mycelial growth of the fungi was evaluated. Scanning Electron Microscopic (SEM) images were obtained to suggest modes of action of the compounds analyzed. The antimycotoxigenic activity was determined by HPLC. A.carbonarius was completely inhibited by cumin EO (500 µL L−1), by laurel EO and by cuminaldehyde (5000 µL L−1). The cumin EO (500 µL L−1) completely inhibited the growth of A.niger. All the samples inhibited the mycelial growth of A.ochraceus, especially cumin EO and cuminaldehyde (250 µL L−1). A.westerdijkiae was completely inhibited by cumin EO and cuminaldehyde (1000 µL L−1), by laurel EO and 1,8-cineole (10000 µL L−1). A decrease in the production of ochratoxin A (OTA) was observed post-treatment, except in A. ochraceus, only inhibited by laurel EO. SEM images showed morphological changes in fungal structures and spore inhibition post-treatment. The results confirmed the antifungal and antimycotoxigenic effect of EO and their principal constituents on fungi evaluated.