Antifungal Activity of the Ethanol Extract from <i>Flos Rosae Chinensis</i> with Activity against Fluconazole‐Resistant Clinical <i>Candida</i>

Zhang, Lulu; Lin, Hui Wen; Liu, Wei; Dai, Bao-Di; Yan, Lan; Cao, Yongbing; Jiang, Yuanying

doi:10.1155/2017/4780746

Cited by 7 publications

(9 citation statements)

References 39 publications

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“… 20 Many plant-derived substances have shown their effectiveness to fight Candida infections, particularly those less susceptible to current antifungal drugs. 45 The use of alcoholic extract of Flos rosae chinensis has been shown to increase the activity of FLZ against the drug-resistant C. albicans . 46 The extract of Punica granatum and its purified compound punicalagin showed a synergistic effect with FLZ.…”

Section: Discussionmentioning

confidence: 99%

Coadministration of Ginger Extract and Fluconazole Shows a Synergistic Effect in the Treatment of Drug-Resistant Vulvovaginal Candidiasis

Khan

Azam

Allemailem

et al. 2021

IDR

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Background Azoles are the most common antifungal drugs used in the treatment of vulvovaginal candidiasis (VVC). The frequency of azole-resistant Candida isolates has increased dramatically in the last two decades. Here, we assessed the antifungal activity of a combination of fluconazole (FLZ) and methanolic extract of ginger (Meth-Gin) against drug-resistant vulvovaginal candidiasis (VVC) in a murine model. Methods The in vitro activity of FLZ or a combination of FLZ and Meth-Gin was determined against Candida albicans by the agar well diffusion, macrodilution, time-kill and the biofilm eradication methods. The therapeutic efficacy of the formulations was assessed by analyzing the fungal load, pro-inflammatory cytokines, percent apoptotic cells and the histological changes in the vaginal tissues of the mice. Moreover, the renal toxicity the drug formulation was evaluated by analyzing the levels of the blood urea nitrogen (BUN) and creatinine. Results The results of in vitro study demonstrated that FLZ did not show any activity against C. albicans , whereas a combination of FLZ and Meth-Gin demonstrated greater activity as shown by the data of the zone of growth inhibition, MIC and time-kill assay. FLZ or Meth-Gin treatment could not completely cure VVC, whereas a combination of FLZ and Meth-Gin was greatly effective in the treatment of VVC. The vaginal tissue from mice of the infected control group had the highest fungal load of 155370 ± 20617 CFUs. Treatment with FLZ at a dose of 40 mg/kg reduced the fungal load to 120863 ± 10723 CFUs. Interestingly, the mice treated with a combination of FLZ (40 mg/kg) and Meth-Gin (200 mg/kg) had a fungal load of 256 ± 152 CFUs. Besides, FLZ and Meth-Gin combination effectively reduced the pro-inflammatory cytokines (IL-1β, TNF-α and IL-17) and the percentage of apoptotic cells in the vaginal tissues. Likewise, the histological analysis revealed the epithelial necrosis, shedding and ulceration in the vaginal tissue, whereas treatment with FLZ and Meth-Gin combination reversed the histopathological changes in the vaginal epithelium and lamina propria. Conclusion The findings of the current study suggest that the co-administration of Meth-Gin and FLZ may have a potential therapeutic effect in the treatment of azole-resistant candidiasis.

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Section: Discussionmentioning

confidence: 99%

Coadministration of Ginger Extract and Fluconazole Shows a Synergistic Effect in the Treatment of Drug-Resistant Vulvovaginal Candidiasis

Khan

Azam

Allemailem

et al. 2021

IDR

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“…, Mahonia aquifolium Nutt. , Glycyrrhiza glabra L. and so on, were reported to exert antifungal activity [8–13]. In our screening study, we found that Maianthemum japonicum ( A.…”

Section: Introductionmentioning

confidence: 87%

Antifungal Activity of Crude Extract from the Rhizome and Root ofSmilacina japonicaA. Gray

Liu

Sun

Yang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate the antifungal activity of hydroalcoholic extract from Smilacina japonica A. Gray (SJA) against different fungi. The minimum inhibitory concentration (MIC) for SJA was determined by the broth microdilution method. The antifungal effects of SJA against Candida albicans were further confirmed by cell growth test and time-kill curve test. The effects of SJA on the fungal morphology and ultrastructure were also evaluated. SJA has a broad-spectrum antifungal activity. The MICs of SJA against different fungi, including fluconazole-sensitive and -resistant Candida albicans, other Candida species, and Cryptococcus neoformans, ranged from 208 μg/ml to 1665 μg/ml. Furthermore, SJA displayed fungicidal activity against varied fungi and obviously inhibited the hyphal growth of fungi. The mechanism study revealed that the antifungal activity of SJA might be associated with its effect on the cell morphology and ultrastructure.

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“…For the time-kill curve experiments, 25 C. albicans ZY23 was cultured in YPD liquid medium for 16 h, and then the fungal suspension (100 μL) was transferred to fresh YPD liquid medium (10 mL). After culturing for another 4 h, the fungi were washed with PBS three times and resuspended in RPMI medium to a final concentration of 1×10 3 CFU/mL.…”

Section: Methodsmentioning

confidence: 99%

Cotreatment with Aspirin and Azole Drugs Increases Sensitivity of Candida albicans in vitro

Feng¹,

Yang²,

Ma³

et al. 2021

IDR

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Purpose This study aimed to investigate the effects of aspirin (acetyl salicylic acid [ASA]) combined with fluconazole (FCA), itraconazole (ITR), or voriconazole (VRC) on Candida albicans under planktonic and biofilm conditions. Methods A total of 39 clinical C. albicans strains were used to perform the in vitro drug sensitivity assay under different conditions using the M27-A4 broth microdilution method. The minimal inhibitory concentrations (MICs) and fractional inhibitory concentration index (FICI) values were calculated. C. albicans ZY23 was chosen for the further analyses. Results Under planktonic conditions, the half maximal MIC (MIC 50 ) values of FCA, ITR, and VRC were 64–0.5 μg/mL, 32–0.0625 μg/mL, and 16–0.125 μg/mL, respectively, when applied, whereas in combination with ASA, the values decreased to 32–0.25 μg/mL, 8–0.0313 μg/mL, and 8–0.0313 μg/mL, respectively. Under biofilm conditions, FCA, ITR, or VRC alone showed MIC 50 values of 128–8 μg/mL, 32–4 μg/mL, and 32–0.5 μg/mL, whereas in combination with ASA the values were decreased to 32–0.5 μg/mL, 16–0.5 μg/mL, and 8–0.0625 μg/mL, respectively. Analysis of the FICI showed that the sensitization rate of ASA to FCA, ITR, and FCA under planktonic conditions was 43.59%, whereas the sensitization rates of ASP to FCA, ITR, and FCA under biofilm conditions were 46.15%, 46.15%, and 48.72%, respectively. Additionally, the time-growth and time-kill curves of C. albicans ZY23 further verified the synergistic effects of ASA on azole drugs. Conclusion ASA may act as an enhancer of the inhibitory effects of azole drugs on the growth of clinical C. albicans under planktonic and biofilm conditions.

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Antifungal Activity of the Ethanol Extract from Flos Rosae Chinensis with Activity against Fluconazole‐Resistant Clinical Candida

Cited by 7 publications

References 39 publications

Coadministration of Ginger Extract and Fluconazole Shows a Synergistic Effect in the Treatment of Drug-Resistant Vulvovaginal Candidiasis

Coadministration of Ginger Extract and Fluconazole Shows a Synergistic Effect in the Treatment of Drug-Resistant Vulvovaginal Candidiasis

Antifungal Activity of Crude Extract from the Rhizome and Root ofSmilacina japonicaA. Gray

Cotreatment with Aspirin and Azole Drugs Increases Sensitivity of Candida albicans in vitro

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