Antifungal Activity of Volatile Components from Ceratocystis fimbriata and Its Potential Biocontrol Mechanism on Alternaria alternata in Postharvest Cherry Tomato Fruit

Xing, Shijun; Gao, Yating; Li, Xue; Ren, Huan; Gao, Yang; Yang, Hui; Liu, Yanmei; ShuQi, He; Huang, Qiwei

doi:10.1128/spectrum.02713-22

Cited by 17 publications

(8 citation statements)

References 56 publications

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“…Arg381, Tyr118, and His468 are the active sites of antifungal proteins. Binding energies in Kcal/mol are primarily negative and do not exceed the − 5.000 kcal/mol threshold, demonstrating molecular stability 52 . These results are consistent with those of Jeddi et al 53 , who demonstrated antioxidant and antibacterial effects toward the same targeted receptors, NADPH oxidase and FimH lectin proteins, for two major compounds of the essential oil extracted from the mill of Lavandula angustifolia.…”

Section: Resultsmentioning

confidence: 93%

Comprehensive analysis of different solvent extracts of Ferula communis L. fruit reveals phenolic compounds and their biological properties via in vitro and in silico assays

Nouioura,

El fadili,

El Barnossi

et al. 2024

Sci Rep

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Although giant fennel is recognized as a “superfood” rich in phytochemicals with antioxidant activity, research into the antibacterial properties of its fruits has been relatively limited, compared to studies involving the root and aerial parts of the plant. In this study, seven solvents—acetone, methanol, ethanol, ethyl acetate, chloroform, water, and hexane—were used to extract the chemical constituents of the fruit of giant fennel (Ferula communis), a species of flowering plant in the carrot family Apiaceae. Specific attributes of these extracts were investigated using in silico simulations and in vitro bioassays. High-performance liquid chromatography equipped with a diode-array detector (HPLC–DAD) identified 15 compounds in giant fennel extract, with p-coumaric acid, 3-hydroxybenzoic acid, sinapic acid, and syringic acid being dominant. Among the solvents tested, ethanol demonstrated superior antioxidant activity and phenolic and flavonoid contents. F. communis extracts showed advanced inhibition of gram-negative pathogens (Escherichia coli and Proteus mirabilis) and variable antifungal activity against tested strains. Molecular docking simulations assessed the antioxidative, antibacterial, and antifungal properties of F. communis, facilitating innovative therapeutic development through predicted compound–protein interactions. In conclusion, the results validate the ethnomedicinal use and potential of F. communis. This highlights its significance in natural product research and ethnopharmacology.

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Section: Resultsmentioning

confidence: 93%

Comprehensive analysis of different solvent extracts of Ferula communis L. fruit reveals phenolic compounds and their biological properties via in vitro and in silico assays

Nouioura,

El fadili,

El Barnossi

et al. 2024

Sci Rep

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“…A total of 129 and 130 VOCs were detected at five and nine days, respectively, in the range of measured masses (mass protonated range m / z = 20–300) after the subtraction of peaks associated with the PTR-MS ion source and their isotopes. VOCs’ putative identification is reported in Supplementary Table S2 [ 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. Overall, all samples’ released VOCs were blends composed of similar compounds, but in different proportions.…”

Section: Resultsmentioning

confidence: 99%

Trichoderma harzianum Volatile Organic Compounds Regulated by the THCTF1 Transcription Factor Are Involved in Antifungal Activity and Beneficial Plant Responses

Rubio

Monti

Gualtieri

et al. 2023

JoF

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The transcription factor THCTF1 from Trichoderma harzianum, previously linked to the production of 6-pentyl-2H-pyran-2-one (6-PP) derivatives and antifungal activity against Fusarium oxysporum, has been related in this study to conidiation, production of an array of volatile organic compounds (VOCs) and expression of methyltransferase genes. VOCs emitted by three T. harzianum strains (wild type T34, transformant ΔD1-38 that is disrupted in the Thctf1 gene encoding the transcription factor THCTF1, and ectopic integration transformant ΔJ3-16) were characterized by Proton Transfer Reaction-Quadrupole interface-Time-Of-Flight-Mass Spectrometry (PTR-Qi-TOF-MS). Thctf1 disruption affected the production of numerous VOCs such as the antifungal volatiles 2-pentyl furan and benzaldehyde which were under-emitted, and acetoine, a plant systemic defense inductor, which was over-emitted. Biological assays show that VOCs regulated by THCTF1 are involved in the T. harzianum antifungal activity against Botrytis cinerea and in the beneficial effects leading to Arabidopsis plant development. The VOC blend from the disruptant ΔD1-38: (i) inhibited Arabidopsis seed germination for at least 26 days and (ii) when applied to Arabidopsis seedlings resulted in increased jasmonic acid- and salicylic acid-dependent defenses.

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“…Compound 1,8-dimethoxynaphthalene (37, synthesized from commercial 1,8-dihydroxynaphthalene) and a mixture of terpenes (commercial standard item containing β-caryophyllene) were tested at different concentrations to evaluate the effect on M. citricolor pseudopilei (Figure 4A,B). The mixture of terpenes (20,200, and 2000 μg) did not produce any effect over the pseudopilei. On the other hand, the pseudopilei enclosed in the Petri dishes along with the filter discs loaded with 500 and 1000 μg of the compound 1,8dimethoxynaphthalene (37) experienced the "burned-like" effect after 24 h at room temperature, which was similar to the damage observed when the pathogen was confronted against D. eschscholtzii GU11N (Figure 4C).…”

Section: ■ Introductionmentioning

confidence: 81%

“…Polydimethylsiloxane (PDMS) is the commonly used nonpolar coating for SPME, thanks to its thermal stability and nonpolar affinity . In recent studies on VOCs produced by fungi, the PDMS fiber has been the preferred option for their detection. − …”

Section: Introductionmentioning

confidence: 99%

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Differential Volatile Organic Compound Expression in the Interaction ofDaldinia eschscholtziiandMycena citricolor

Escudero-Leyva,

Quirós-Guerrero,

Vásquez-Chaves

et al. 2023

ACS Omega

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Fungi exhibit a wide range of ecological guilds, but those that live within the inner tissues of plants (also known as endophytes) are particularly relevant due to the benefits they sometimes provide to their hosts, such as herbivory deterrence, disease protection, and growth promotion. Recently, endophytes have gained interest as potential biocontrol agents against crop pathogens, for example, coffee plants (Coffea arabica). Published results from research performed in our laboratory showed that endophytic fungi isolated from wild Rubiaceae plants were effective in reducing the effects of the American leaf spot of coffee (Mycena citricolor). One of these isolates (GU11N) from the plant Randia grandifolia was identified as Daldinia eschscholtzii (Xylariales). Its antagonism mechanisms, effects, and chemistry against M. citricolor were investigated by analyzing its volatile profile alone and in the presence of the pathogen in contactless and dual culture assays. The experimental design involved direct sampling of agar plugs in vials for headspace (HS) and headspace solid-phase microextraction (HS-SPME) gas chromatography-mass spectrometry (GC-MS) analysis. Additionally, we used ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS) to identify nonvolatile compounds from organic extracts of the mycelia involved in the interaction. Results showed that more volatile compounds were identified using HS-SPME (39 components) than those by the HS technique (13 components), sharing only 12 compounds. Statistical tests suggest that D. eschscholtzii inhibited the growth of M. citricolor through the release of VOCs containing a combination of 1,8-dimethoxynapththalene and terpene compounds affecting M. citricolor pseudopilei. The damaging effects of 1,8-dimethoxynaphthalene were corroborated in an in vitro test against M. citricolor pseudopilei; scanning electron microscopy (SEM) photographs confirmed structural damage. After analyzing the UHPLC-HRMS/MS data, a predominance of fatty acid derivatives was found among the putatively identified compounds. However, a considerable proportion of features (37.3%) remained unannotated. In conclusion, our study suggests that D. eschscholtzii has potential as a biocontrol agent against M. citricolor and that 1,8-dimethoxynaphthalene contributes to the observed damage to the pathogen’s reproductive structures.

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Antifungal Activity of Volatile Components from Ceratocystis fimbriata and Its Potential Biocontrol Mechanism on Alternaria alternata in Postharvest Cherry Tomato Fruit

Abstract: In this research, the volatile organic compounds (VOCs) produced based on C. fimbriata exhibited strong antifungal activity against the fungal pathogen A. alternata . Our aim is to explore their bacteriostatic components.

Cited by 17 publications

References 56 publications

Comprehensive analysis of different solvent extracts of Ferula communis L. fruit reveals phenolic compounds and their biological properties via in vitro and in silico assays

Comprehensive analysis of different solvent extracts of Ferula communis L. fruit reveals phenolic compounds and their biological properties via in vitro and in silico assays

Trichoderma harzianum Volatile Organic Compounds Regulated by the THCTF1 Transcription Factor Are Involved in Antifungal Activity and Beneficial Plant Responses

Differential Volatile Organic Compound Expression in the Interaction ofDaldinia eschscholtziiandMycena citricolor

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