Antifungal activity of volatile compounds produced by Staphylococcus sciuri strain MarR44 and its potential for the biocontrol of Colletotrichum nymphaeae, causal agent strawberry anthracnose

Alijani, Zahra; Amini, Jahanshir; Ashengroph, Morahem; Bahramnejad, Bahman

doi:10.1016/j.ijfoodmicro.2019.108276

Cited by 65 publications

(57 citation statements)

References 63 publications

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“…S. saprophyticus L-38 inhibited A. flavus conidial germination and mycelial proliferation, through the production of 3,3-dimethyl-1,2-epoxybutane [48]. The inhibition of the mycelial growth and conidial germination of the plant pathogen Colletotrichum nymphaeae by Staphylococcus sciuri MarR44 has been also attributed mainly to volatile compounds, but without ruling out the role of other metabolites such as siderophore, chitinase, protease, hydrogen cyanide, indole-3-acetic acid, and gibberellin and the biofilm formation [49]. In this sense, the production of extracellular metabolites, including volatile compounds, proteins, lytic enzymes or organic acids has been reported in a range of microorganisms with antifungal activity [44,[50][51][52].…”

Section: Discussionmentioning

confidence: 99%

Selection and Evaluation of Staphylococcus xylosus as a Biocontrol Agent against Toxigenic Moulds in a Dry-Cured Ham Model System

et al. 2020

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Toxigenic moulds can develop on the surface of dry-cured meat products during ripening due to their ecological conditions, which constitutes a risk for consumers. A promising strategy to control this hazard is the use of antifungal microorganisms usually found in these foods. However, to date, the effectiveness of gram-positive catalase-positive cocci (GCC+) has not been explored. The aim of this work was to select GCC+ isolates with antifungal activity to study its effectiveness in a dry-cured ham model system at the environmental conditions reached during the ripening. Forty-five strains of GCC+ were evaluated and the isolate Staphylococcus xylosus Sx8 was selected to assess its efficacy at two different concentrations (106 and 104 cfu/mL) against Penicillium nordicum, Aspergillus flavus, Aspergillus parasiticus, and Penicillium griseofulvum at 15, 20, and 25 °C. The results showed that the inoculation of 106 cfu/mL of S. xylosus completely inhibited the growth of most fungi. In addition, in the presence of this strain at 104 cfu/mL, a significant reduction in fungal growth and mycotoxins production was observed at the three temperatures studied. In conclusion, S. xylosus Sx8 possesses great potential as a biological agent to control toxigenic moulds in dry-cured meat products.

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Section: Discussionmentioning

confidence: 99%

Selection and Evaluation of Staphylococcus xylosus as a Biocontrol Agent against Toxigenic Moulds in a Dry-Cured Ham Model System

et al. 2020

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“…The antagonism of fungal pathogens by microorganisms mainly depends on their secretion of antimicrobial active compounds [11], competition for nutrients and living space [12], hyperparasitism and induction of systemic resistance responses in the host [13], and secretion of cell wall degrading enzymes (CWDEs) [14]. Additionally, volatile organic compounds (VOCs) released by microorganisms receive attention due to their antimicrobial effects [15,16]. Recently, VOCs produced by microorganisms were proposed as an alternative control method for fungal plant diseases because they can influence pathogenic fungal growth, deforming fungal hyphae and spores [17].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Volatile Organic Compounds Emitted from Endophytic Burkholderia cenocepacia ETR-B22 by SPME-GC-MS and Their Inhibitory Activity against Various Plant Fungal Pathogens

et al. 2020

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The use of antagonistic microorganisms and their volatile organic compounds (VOCs) to control plant fungal pathogens is an eco-friendly and promising substitute for chemical fungicides. In this work, endophytic bacterium ETR-B22, isolated from the root of Sophora tonkinensis Gagnep., was found to exhibit strong antagonistic activity against 12 fungal pathogens found in agriculture. Strain ETR-B22 was identified as Burkholderia cenocepacia based on 16S rRNA and recA sequences. We evaluated the antifungal activity of VOCs emitted by ETR-B22. The VOCs from strain ETR-B22 also showed broad-spectrum antifungal activity against 12 fungal pathogens. The composition of the volatile profiles was analyzed based on headspace solid phase microextraction (HS-SPME) gas chromatography coupled to mass spectrometry (GC-MS). Different extraction strategies for the SPME process significantly affected the extraction efficiency of the VOCs. Thirty-two different VOCs were identified. Among the VOC of ETR-B22, dimethyl trisulfide, indole, methyl anthranilate, methyl salicylate, methyl benzoate, benzyl propionate, benzyl acetate, 3,5-di-tert-butylphenol, allyl benzyl ether and nonanoic acid showed broad-spectrum antifungal activity, and are key inhibitory compounds produced by strain ETR-B22 against various fungal pathogens. Our results suggest that the endophytic strain ETR-B22 and its VOCs have high potential for use as biological controls of plant fungal pathogens.

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“…The methods for prevention and control of banana fusarium include chemical control (Bauer et al 2016), breeding of resistant varieties (Ambrico and Trupo 2017), agricultural control measures (Ben Abdallah et al 2018) and biological control (Alijani et al 2019). The first three measures have disadvantages, such as environmental pollution, high costs and unstable control effects (Jeong et al 2016;Hennessy et al 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The basis of biological control is the screening of highly effective biocontrol strains (Qu et al 2016). Many studies have reported screening biocontrol bacteria, including Streptomyces (Xue et al 2013), Bacillus (Keikha et al 2015), Pseudomonas (Abidin et al 2016), Trichoderma (Suksaard et al 2017) and nonpathogenic F. oxysporum (Alijani et al 2019), which can prevent and control fusarium wilt. China started late in the research on banana fusarium wilt prevention.…”

Section: Introductionmentioning

confidence: 99%

“…Karimi et al (2017) showed that the antibacterial protein produced by Bacillus subtilis B25 metabolism can make F. oxysporum hyphae or spores swell and twist or deform, its contents can be clearly seen, and the growth and reproduction of pathogenic bacteria are inhibited. Streptomyces grow by attaching to and invading the mycelium of pathogenic bacteria, and release active substances to cause host granulation of the pathogenic bacteria and coagulation of protoplasts, eventually causing the mycelia of the pathogenic bacteria to dissolve (Alijani et al 2019). In addition, Streptomyces can secrete a variety of cell wall hydrolases, such as chitinase (Pageni et al 2014), glucanase (Passari et al 2015), cellulose (Singh and Gaur 2016), nuclease (He et al 2020) and so on.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal mechanism of Streptomyces ma. FS‐4 on fusarium wilt of banana

Duan

Chen

Pang

et al. 2020

J. Appl. Microbiol.

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Aim: Research on prevention and cure of banana wilt is important to ensure the healthy development of the banana industry. In this study, antifungal mechanism of Streptomyces ma. FS-4 on fusarium wilt of banana was investigated. Methods and Results: The physiological strain of banana fusarium pathogen Fusarium oxysporum f. sp. cubense Race 4 (FOC.4) was used as the target fungus, and the antifungal mechanism of the crude extract of Streptomyces ma. FS-4 was investigated. Eighteen different compounds identified by gas chromatography-mass spectrometry were composed of aldehydes, methyl, hydrocarbons, amides, esters and acids. FS-4 significantly inhibited the spore germination of the target fungi, with an EC 50 of 22Á78 μg ml −1. After treatment with 100 μg ml −1 FS-4 crude extract, the N-acetylglucosamine content in the mycelium increased 1Á95-fold. However, the extract had no significant effect on β-1,3-glucanase. At the FS-4 crude extract dose of 100 μg ml −1 , the total sugar and protein contents decreased by 28Á6 and 29Á1% respectively, and the fat content was 41Á3%. FS-4 significantly inhibited the activity of the mitochondrial complex III of Foc4, which was reduced by 52Á45%. Moreover FS-4 reduced the activity of succinate dehydrogenase, a key enzyme in the Krebs cycle, by 60Á2%. However, FS-4 had no significant effect on malate dehydrogenase. The membrane potential on the mitochondrial inner membrane was significantly reduced at the test concentration of 100 μg ml −1. ROS gradually accumulated in the Foc4 hypha, and the burst was 3Á97 times higher than the control. Conclusions: This study demonstrated that the antifungal mechanism of Streptomyces ma. FS-4 against Foc4 includes the destruction of the plasma membrane and mitochondrial dysfunction and finally induction of cell apoptosis. Significance and Impact of the Study: These results may indicate the prevention and control of banana wilt, which is of great significance to the healthy development of banana industry system.

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Antifungal activity of volatile compounds produced by Staphylococcus sciuri strain MarR44 and its potential for the biocontrol of Colletotrichum nymphaeae, causal agent strawberry anthracnose

Cited by 65 publications

References 63 publications

Selection and Evaluation of Staphylococcus xylosus as a Biocontrol Agent against Toxigenic Moulds in a Dry-Cured Ham Model System

Selection and Evaluation of Staphylococcus xylosus as a Biocontrol Agent against Toxigenic Moulds in a Dry-Cured Ham Model System

Characterization of Volatile Organic Compounds Emitted from Endophytic Burkholderia cenocepacia ETR-B22 by SPME-GC-MS and Their Inhibitory Activity against Various Plant Fungal Pathogens

Antifungal mechanism of Streptomyces ma. FS‐4 on fusarium wilt of banana

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