Antifungal and anticancer effects of a polysaccharide-protein complex from the gut bacterium<i>Raoultella ornithinolytica</i>isolated from the earthworm<i>Dendrobaena veneta</i>

Fiołka, Marta J.; Lewtak, Kinga; Rzymowska, Jolanta; Grzywnowicz, Krzysztof; Hułas-Stasiak, Monika; Sofińska-Chmiel, Weronika; Skrzypiec, Krzysztof

doi:10.1111/2049-632x.12056

Cited by 17 publications

(20 citation statements)

References 57 publications

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“…This is not the first observation of the selective action of compounds derived from D. veneta earthworm against tumor cells but not against normal cells. Earlier reports demonstrated the selective anticancer activity (toward HeLa, T47D, and TOV-112D cell lines) of complexes obtained from metabolites produced by symbiotic intestinal bacteria of this species (40,41). However, the metabolites were obtained outside the earthworm body in laboratory conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The MTT cytotoxicity assay was used to determine the cytotoxicity of unheated and heated (40,50,60,70,80, and 90°C for 10 min) CF. A549 non-small cell lung carcinoma from the European Collection of Cell Cultures (ECACC, Salisbury, UK) catalog number 86012804 and BEAS-2B bronchial epithelial cell line ATCC CRL-9609 kindly provided by Dr. D.…”

Section: Mtt Cytotoxicity Assaymentioning

confidence: 99%

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Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Fiołka¹,

Rzymowska

Bilska

et al. 2019

APMIS

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It is known that earthworm coelomic fluid (CF) can affect not only cancer but also normal cells. The study demonstrated that the CF of the earthworm Dendrobaena veneta exhibited cytotoxicity against A549 lung cancer cells but did not toward the bronchial epithelial cell line BEAS‐2B. The selective effect on the tumor cells was achieved after a short‐term CF heat pre‐treatment at 70 °C. The cytotoxic effect of the CF was time‐ and concentration‐dependent. The CF noticeably decreased the viability and affected the morphology of the A549 cells. Scanning electron microscopy revealed a different degree of destruction of the nucleus and cytoplasm of A549 cells. As determined by atomic force microscopy, the cell surface roughness increased while the cell stiffness was reduced upon the CF treatment. A twofold increase in the caspase 3, 4, 5, and 10 levels was observed in the A549 cells after the incubation with the CF. The results obtained by flow cytometry using Annexin V confirmed the proapoptotic effect of the earthworm CF on A549 lung cancer cells. The D. veneta CF and active fraction obtained with cytotoxicity toward A549 lung cancer is an interesting and promising preparation for further biological, chemical, and biomedical research.

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Section: Discussionmentioning

confidence: 99%

Section: Mtt Cytotoxicity Assaymentioning

confidence: 99%

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Fiołka¹,

Rzymowska

Bilska

et al. 2019

APMIS

Self Cite

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“…In addition, higher concentrations of earthworm extracts are nontoxic and have excellent antiinflammatory and antipyretic properties [7] . Further researches have revealed the potential for using earthworm extracts as antibacterial [8] , antifungal [9] , antiviral [10] and antitumor [8,9] agents. In this study, earthworms were studied to determine their active components used in the traditional prescriptions as starting materials.…”

Section: Research Papermentioning

confidence: 99%

Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A from Eisenia foetida on Breast Cancer Cell Line MCF-7

Liu¹,

Chen²,

Pan³

et al. 2017

pharmaceutical-sciences

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Liu, et al.: Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A on MCF-7Earthworm fibrinolytic enzyme from Chinese earthworm Eisenia foetida was isolated to investigate its antitumor activity in breast cancer cells. The protein isolated was characterised as earthworm fibrinolytic enzyme using the fibrin plate method. The molecular weight of earthworm fibrinolytic enzyme component was determined to be 25 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis assay using a standard protein ladder. Earthworm fibrinolytic enzyme can markedly inhibit the growth and migration of MCF-7 cells in a dose and time-dependent manner. In addition, MCF-7 cells treated with earthworm fibrinolytic enzyme (40 μg/ml) began to undergo apoptosis after 24 h. Expression of focal adhesion kinase and CD44v6 measured using reverse transcription polymerase chain reaction and Western blot was down-regulated in a concentration-dependent manner (20-80 μg/ml), resulting in the suppression of MCF-7 cells adhesion. The obtained earthworm fibrinolytic enzyme displayed an antitumor effect on MCF-7 cells in vitro, revealing the therapeutic potential of E. foetida.

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“…Regarding its anti-cancer activity, the polysaccharide-protein complex isolated from metabolites of bacteria inhibiting gut of Raoultella ornithinolytica earthworms have anti-fungal and anticancer activity against breast ductal carcinoma and endometrioid ovarian cancer in vitro (Fiołka et al 2013). Furthermore, earthworm aqueous extracts have been utilized in the green synthesis of gold nanoparticles (AuNPs).…”

Section: Earthwormsmentioning

confidence: 99%

Helminthes and insects: maladies or therapies

El-Tantawy

2014

Parasitol Res

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By definition, parasites cause harm to their hosts. But, considerable evidence from ancient traditional medicine has supported the theory of using parasites and their products in treating many diseases. Maggots have been used successfully to treat chronic, long-standing, infected wounds which failed to respond to conventional treatment by many beneficial effects on the wound including debridement, disinfection, and healing enhancement. Maggots are also applied in forensic medicine to estimate time between the death and discovery of a corpse and in entomotoxicology involving the potential use of insects as alternative samples for detecting drugs and toxins in death investigations. Leeches are segmented invertebrates, famous by their blood-feeding habits and used in phlebotomy to treat various ailments since ancient times. Leech therapy is experiencing resurgence nowadays in health care principally in plastic and reconstructive surgery. Earthworms provide a source of medicinally useful products with potential antimicrobial, antiviral, and anticancer properties. Lumbrokinases are a group of fibrinolytic enzymes isolated and purified from earthworms capable of degrading plasminogen-rich and plasminogen-free fibrin and so can be used to treat various conditions associated with thrombotic diseases. Helminth infection has been proved to have therapeutic effects in both animal and human clinical trials with promising evidence in treating many allergic diseases and can block the induction of or reduce the severity of some autoimmune disorders as Crohn's disease or ulcerative colitis. What is more, venomous arthropods such as scorpions, bees, wasps, spiders, ants, centipedes, snail, beetles, and caterpillars. The venoms and toxins from these arthropods provide a promising source of natural bioactive compounds which can be employed in the development of new drugs to treat diseases as cancer. The possibility of using these active molecules in biotechnological processes can make these venoms and toxins a valuable and promising source of natural bioactive compounds. The therapeutic use of helminthes and insects will be of great value in biomedicine and further studies on insect toxins will contribute extensively to the development of Biomedical Sciences.

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Antifungal and anticancer effects of a polysaccharide-protein complex from the gut bacteriumRaoultella ornithinolyticaisolated from the earthwormDendrobaena veneta

Cited by 17 publications

References 57 publications

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Antitumor activity and apoptotic action of coelomic fluid from the earthworm Dendrobaena veneta against A549 human lung cancer cells

Antitumor Studies of Earthworm Fibrinolytic Enzyme Component A from Eisenia foetida on Breast Cancer Cell Line MCF-7

Helminthes and insects: maladies or therapies

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