Antifungal and antimicrobial proteins and peptides of potato (Solanum tuberosum L.) tubers and their applications

Bártová, Veronika; Bárta, Jan; Jarošová, Markéta

doi:10.1007/s00253-019-09887-9

Cited by 55 publications

(32 citation statements)

References 105 publications

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“…The results of another study that also coincide with our findings is the study by Bártová et al [ 17 ] who reported that the protease inhibitors of potato I and II reduced the growth of P. infestans, Rhizoctonia solani , and Botrytis cinerea and of the fungi of the genus Fusarium . These inhibitors were also able to inhibit certain microorganisms such as Staphylococcus aureus, Listeria monocytogenes, Escherichia coli , and Candida albicans .…”

Section: Discussionsupporting

confidence: 92%

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175^TM: A Comparative In Vitro Study

Enciso

Medina

Mauricio

et al. 2020

International Journal of Dentistry

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Objective. To determine the in vitro antibacterial effect of four concentrations of the hydroalcoholic extract of Solanum tuberosum “tocosh” (HET) against Streptococcus mutans ATCC 25175TM. Methods. This was a prospective, experimental, comparative study. Fermented tocosh was subjected to hydric stress to obtain a hydroalcoholic extract at four different concentrations: 100%, 50%, 75%, and 25%. S. mutans strains were cultured in brain heart infusion agar using the swab technique. The antibacterial effectiveness of HET was evaluated following the Kirby–Bauer disk diffusion method and compared with 0.12% chlorhexidine (positive control group). Results. The highest mean inhibitory effect was achieved with HET at 100% (33.1 ± 2.2 mm, showing a gradual reduction in the other HET groups at 75%, 50%, and 25% (29.7 ± 1.3 mm, 26.6 ± 2.0, and 20.1 ± 1.8 mm, respectively)). Inferential analysis found statistically significant differences among all the experimental groups (p=0.001). The post hoc analysis also showed significant differences among all the experimental groups evaluated; however, there were no significant differences between HET 50% and chlorhexidine 0.12% (p>0.05). Conclusions. It was found that the highest antibacterial effectiveness was obtained by HET 100%, being even higher than the 0.12% chlorhexidine positive control, and was statistically significant. Post hoc analysis showed that almost all the concentrations showed optimal efficacy against S. mutans.

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Section: Discussionsupporting

confidence: 92%

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175^TM: A Comparative In Vitro Study

Enciso

Medina

Mauricio

et al. 2020

International Journal of Dentistry

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“…Bacteria use patatins to target host cell membrane as effectors via the type III secretion system ( Finck-Barbançon et al, 1997 ; Phillips et al, 2003 ; Sato et al, 2003 ) and are activated by ubiquitin ( Anderson et al, 2015 ). The eukaryotic patatins are known to have antimicrobial activities (e.g., Phytophthora infestans inhibition) ( Bártová et al, 2019 ). Tomato and potato, belonging to the family Solanaceae , interact with microbes via patatin and patatin-like proteins, and we will further explore plant-microbe interactions mediated by these proteins.…”

Section: Resultsmentioning

confidence: 99%

Testing the Two-Step Model of Plant Root Microbiome Acquisition Under Multiple Plant Species and Soil Sources

et al. 2020

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The two-step model for plant root microbiomes considers soil as the primary microbial source. Active selection of the plant’s bacterial inhabitants results in a biodiversity decrease toward roots. We collected sixteen samples of in situ ruderal plant roots and their soils and used these soils as the main microbial input for single genotype tomatoes grown in a greenhouse. Our main goal was to test the soil influence in the structuring of rhizosphere microbiomes, minimizing environmental variability, while testing multiple plant species. We massively sequenced the 16S rRNA and shotgun metagenomes of the soils, in situ plants, and tomato roots. We identified a total of 271,940 bacterial operational taxonomic units (OTUs) within the soils, rhizosphere and endospheric microbiomes. We annotated by homology a total of 411,432 (13.07%) of the metagenome predicted proteins. Tomato roots did follow the two-step model with lower α-diversity than soil, while ruderal plants did not. Surprisingly, ruderal plants are probably working as a microenvironmental oasis providing moisture and plant-derived nutrients, supporting larger α-diversity. Ruderal plants and their soils are closer according to their microbiome community composition than tomato and its soil, based on OTUs and protein comparisons. We expected that tomato β-diversity clustered together with their soil, if it is the main rhizosphere microbiome structuring factor. However, tomato microbiome β-diversity was associated with plant genotype in most samples (81.2%), also supported by a larger set of enriched proteins in tomato rhizosphere than soil or ruderals. The most abundant bacteria found in soils was the Actinobacteria Solirubrobacter soli , ruderals were dominated by the Proteobacteria Sphingomonas sp. URGHD0057, and tomato mainly by the Bacteroidetes Ohtaekwangia koreensis , Flavobacterium terrae, Niastella vici , and Chryseolinea serpens. We calculated a metagenomic tomato root core of 51 bacterial genera and 2,762 proteins, which could be the basis for microbiome-oriented plant breeding programs. We attributed a larger diversity in ruderal plants roots exudates as an effect of the moisture and nutrient acting as a microbial harbor. The tomato and ruderal metagenomic differences are probably due to plant domestication trade-offs, impacting plant-bacteria interactions.

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“…The eukaryotic patatins are known to have antimicrobial activities (e.g., Phytophthora infestans inhibition) (Bártová et al, 2019). Tomato and potato, both belonging to the family Solanaceae, interact with microbes via patatin and patatin-like proteins, and we will further explore plant-microbe interactions mediated by these proteins.…”

Section: Discussionmentioning

confidence: 99%

Testing the two-step model of plant root microbiome acquisition under multiple plant species and soil sources

Barajas

Martínez-Sánchez

Romero

et al. 2020

Preprint

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The two-step model for plant root microbiomes considers soil as the primary microbial source. Active selection of the plant's bacterial inhabitants results in a biodiversity decrease towards roots. We collected in situ ruderal plant roots and their soils and used these soils as the main microbial input for single genotype tomatoes grown in a greenhouse. We massively sequenced the 16S rRNA and shotgun metagenomes of the soils, in situ plants, and tomato roots. Tomato roots did follow the two-step model, while ruderal plants did not. Ruderal plants and their soils are closer than tomato and its soil, based on protein comparisons. We calculated a metagenomic tomato root core of 51 bacterial genera and 2,762 proteins, which could be the basis for microbiome-oriented plant breeding programs. The tomato and ruderal metagenomic differences are probably due to plant domestication trade-offs, impacting plantmicrobe interactions.

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Antifungal and antimicrobial proteins and peptides of potato (Solanum tuberosum L.) tubers and their applications

Cited by 55 publications

References 105 publications

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175^TM: A Comparative In Vitro Study

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175^TM: A Comparative In Vitro Study

Testing the Two-Step Model of Plant Root Microbiome Acquisition Under Multiple Plant Species and Soil Sources

Testing the two-step model of plant root microbiome acquisition under multiple plant species and soil sources

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Antifungal and antimicrobial proteins and peptides of potato (Solanum tuberosum L.) tubers and their applications

Cited by 55 publications

References 105 publications

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study

Testing the Two-Step Model of Plant Root Microbiome Acquisition Under Multiple Plant Species and Soil Sources

Testing the two-step model of plant root microbiome acquisition under multiple plant species and soil sources

Contact Info

Product

Resources

About

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175^TM: A Comparative In Vitro Study

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175^TM: A Comparative In Vitro Study