Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of
            <i>Markhamia tomentosa</i>

Ibrahim, Mutiat B.; Kaushik, Nutan; Sowemimo, Abimbola; Chhipa, Hemraj; Koekemoer, Trevor; Venter, Maryna van de; Odukoya, Onatolu

doi:10.1080/13880209.2016.1263671

Cited by 34 publications

(23 citation statements)

References 34 publications

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“…From the processed leaves, endophytic fungal isolation was carried out on potato dextrose agar (PDA) (potato infusion form 200 g/L, dextrose 20 g/L, agar 15 g/L, pH 5.6 ± 0.2) as per previous method. 30 Here, the last wash of surface sterilization was spread plated on PDA as control to check the surface sterility. Both the isolation and control plates were further incubated at 28 C for 10 days and the obtained fungal isolates were purified by sub-culturing.…”

Section: Endophytic Fungi Isolationmentioning

confidence: 99%

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Aswani

Jasim

Vishnu

et al. 2020

Biotechnology Progress

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In the study, endophytic fungi isolated from Ophiorrhiza mungos were screened for camptothecin (CPT) biosynthetic potential by high performance liquid chromatography (HPLC). Among the 16 fungi screened, OmF3, OmF4, and OmF6 were identified to synthesize CPT. Further LC-MS analysis also showed the presence of CPT specific m/z of 349 for the extracts from OmF3, OmF4, and OmF6. However, the fragmentation masses with m/z of 320, 305, 277 and 220 specific to the CPT could be identified only for the OmF3 and OmF4. These CPT producing fungi were further identified as Meyerozyma sp. OmF3 and Talaromyces sp. OmF4. The cultures of these two fungi were then supplemented with nanoparticles and analyzed for the quantitative enhancement of CPT production by LC-MS/MS. From the result, Meyerozyma sp. OmF3 was found to produce 947.3 ± 12.66 μg/L CPT, when supplemented with 1 μg/ mL zinc oxide nanoparticles and the same for uninduced parental strain OmF3 was only 1.77 ± 0.13 μg/L. At the same time, Talaromyces sp. OmF4 showed the highest production of 28.97 ± 0.37 μg/L of CPT when cultured with 10 μg/mL silver nanoparticles and the same for uninduced strain was 1.19 ± 0.24 μg/L. The observed quantitative enhancement of fungal CPT production is highly interesting as it is a rapid and cost effective method. The study is remarkable due to the identification of novel fungal sources for CPT production and its enhancement by nanoparticle supplementation.

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Section: Endophytic Fungi Isolationmentioning

confidence: 99%

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Aswani

Jasim

Vishnu

et al. 2020

Biotechnology Progress

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“…In both these assays, formation of an inhibition halo after incubation around the spots reveals the antifungal activity. A dual culture technique has also been used to test the antagonistic effect of a potential antifungal mold against a target mold, in which agar plugs containing the target and the antifungal molds, respectively, were inoculated at the opposite sides over an agar plate [ 70 , 72 ]. Another screening approach corresponds to the investigation of the enzymatic activities required to degrade the cell wall of the fungal target as shown by Tokpah et al (2016) that screened bacteria for control of a rice pathogen, Magnaporthe grisea .…”

Section: Antifungal Microorganisms In Foodmentioning

confidence: 99%

Antifungal Microbial Agents for Food Biopreservation—A Review

et al. 2017

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Food spoilage is a major issue for the food industry, leading to food waste, substantial economic losses for manufacturers and consumers, and a negative impact on brand names. Among causes, fungal contamination can be encountered at various stages of the food chain (e.g., post-harvest, during processing or storage). Fungal development leads to food sensory defects varying from visual deterioration to noticeable odor, flavor, or texture changes but can also have negative health impacts via mycotoxin production by some molds. In order to avoid microbial spoilage and thus extend product shelf life, different treatments—including fungicides and chemical preservatives—are used. In parallel, public authorities encourage the food industry to limit the use of these chemical compounds and develop natural methods for food preservation. This is accompanied by a strong societal demand for ‘clean label’ food products, as consumers are looking for more natural, less severely processed and safer products. In this context, microbial agents corresponding to bioprotective cultures, fermentates, culture-free supernatant or purified molecules, exhibiting antifungal activities represent a growing interest as an alternative to chemical preservation. This review presents the main fungal spoilers encountered in food products, the antifungal microorganisms tested for food bioprotection, and their mechanisms of action. A focus is made in particular on the recent in situ studies and the constraints associated with the use of antifungal microbial agents for food biopreservation.

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“…Antifungal activity has been detected in a diversity of living organisms including microbes, animals and plants, and antifungal peptides have been isolated from them [13,14,15]. For the sake of safety and availability, in our search for antifungal peptides, we have looked more at plants that have long been used as foods.…”

Section: Introductionmentioning

confidence: 99%

Purification of an Antifungal Peptide from Seeds of Brassica oleracea var. gongylodes and Investigation of Its Antifungal Activity and Mechanism of Action

Wang

Zhang

et al. 2019

Molecules

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In this study, a 8.5-kDa antifungal peptide designated as BGAP was purified from the crude extract of the seeds of Brassica oleracea var. gongylodes by employing a protocol that comprised cation exchange chromatography on SP-Sepharose, cation exchange chromatography on Mono S and gel filtration chromatography on Superdex peptide. BGAP showed the highest amino acid sequence similarity to defensin peptides by mass spectrometric analysis. BGAP showed a broad spectrum of antifungal activity with a half maximal inhibitory concentration at 17.33 μg/mL, 12.37 μg/mL, 16.81 μg/mL, and 5.60 μg/mL toward Colletotrichum higginsianum, Exserohilum turcicum, Magnaporthe oryzae and Mycosphaerella arachidicola, respectively. The antifungal activity of BGAP remained stable (i) after heat treatment at 40–100 °C for 15 min; (ii) after exposure to solutions of pH 1–3 and 11–13 for 15 min; (iii) after incubation with solutions containing K+, Ca2+, Mg2+, Mn2+ or Fe3+ ions at the concentrations of 20–150 mmol/L for 2 h; and (iv) following treatment with 10% methyl alcohol, 10% ethanol, 10% isopropanol or 10% chloroform for 2 h. Fluorescence staining experiments showed that BGAP brought about an increase in cell membrane permeability, a rise in reactive oxygen species production, a decrease in mitochondrial membrane potential, and an accumulation of chitin at the hyphal tips of Mycosphaerella arachidicola.

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Antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of Markhamia tomentosa

Cited by 34 publications

References 34 publications

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Nanoelicitor based enhancement of camptothecin production in fungi isolated from Ophiorrhiza mungos

Antifungal Microbial Agents for Food Biopreservation—A Review

Purification of an Antifungal Peptide from Seeds of Brassica oleracea var. gongylodes and Investigation of Its Antifungal Activity and Mechanism of Action

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