Antifungal, antiradical and cytotoxic activities of extractives obtained from Tagetes patula L. (Asteraceae), a potential acaricide plant species

Abstract

Tagetes patula L. shows a complex chemical composition, ranging from glycosylated flavonoids and thiophenes in extracts until terpenoids in the essential oil. In the present study, due to this rich flavonoidic constitution, its antioxidant potential was determined, having shown values of antiradical percentage superior to reference compounds, mainly the extracts prepared with flowers. Previous studies performed emphasized the acaricide potential of T. patula and thus, the present study aimed to verify the action of extractives obtained from aerial parts on growth of entomopathogenic fungi related to biological control of brown dog tick Rhipicephalus sanguineus and the action against pathogenic fungi closely associated with pets. None of the samples inhibited the growth of strains of Beauveria bassiana or Metarhizium anisopliae, enabling feasible future studies of synergism on acaricide activity of formulations containing fungi and extracts. The antimicrobial activity of ethanolic extract of flowers (FlEtOH70%) against Microsporum canis and Trichophyton rubrum was significant (193.3 μg/mL and 253.9 μg/mL, respectively), as well as ethanolic extract from aerial parts (APEtOH70%) against T. rubrum (312.5 μg/mL). In order to ensure the safety of a topical formulation containing the extractives of T. patula, the cytotoxic potential of these samples were tested in murine macrophages cells. At higher concentrations all extracts were quite lethal, with IC50 ranging from 210.96 μg/mL to 468.75 μg/mL for APEtOH70% and FlEtOH70%, respectively. These results suggest that the application of a product containing T. patula extractives in the control of ticks could be used, at principle, only on the environment.

Tagetes spp. Essential Oils and Other Extracts: Chemical Characterization and Biological Activity

“…Politi et al [ 41 ] used 70% (5 mg/mL) ethanolic extracts from the aerial parts (stems, leaves, and flowers) of T. patula to evaluate the antifungal potential of clinically important fungi by microdilution using amphotericin B (16 mg/mL) as a positive control. Extracts from the aerial parts with flowers, without flowers, and with flower extracts were evaluated.…”

“…T. patula presented antioxidant and cytotoxic activity in previous studies; however, the extract did not display good results for antimicrobial action against fungi ( Trichophyton rubrum , Trichophyton mentagrophytes , Microsporum canis , Microsporum anisopliae , and Beauveria bassiana ) [ 41 ]. Conversely, T. minuta essential oil (EO) showed inhibition against Aspergillus niger and Candida albicans in addition to Gram-positive bacteria [ 42 ].…”

Antibacterial, antioxidant and enzyme inhibition activity capacities of Doronicum macrolepis (FREYN&SINT): An endemic plant from Turkey

“…These results suggest that the essential oil of this plant could be used as an antimicrobial agent. In previous studies carried out on similar species, it was reported that the essential oils of the plants showed higher antimicrobial activity when compared to the plant extracts (Politi et al, 2016, Shirazi et al, 2014, Candan et al, 2003).…”

Antineoplastic potential and chemical evaluation of essential oils from leaves and flowers of Tagetes ostenii Hicken

“…After 24 hours of treatment, the viability inhibition of HaCat cells was between 2% to 95.4% for EO 1 and IC50 of 54.45 ng/mL and 3.8% to 96% for EO 2 with an IC50 of 20.83 ng/mL (Figure 1b and c). Other species of the genus, Tagetes patula, also demonstrated cytotoxic potential in a murine macrophage strain for ethanolic extract of the aerial parts and ethanolic extract of flowers (Politi et al 2016).…”