Antifungal Coating Based on Pyocyanin Nanoparticles (Np-Pyo)

Abstract

Pyocyanin is a pigment produced by 95% of Pseudomonas aeruginosa strains and exhibits antimicrobial properties that can be used for different purposes. In this work, PMMA-based nanoparticles that were encapsulated into 200 µg/mL of pyocyanin (Np-Pyo) were produced by the nanoprecipitation method. They were evaluated with respect to antifouling activity against Aspergillus sp. and Penicillium sp. With an encapsulation efficiency of 56%, the NpPyo remained stable for 90 days. Their characteristics were satisfactory for the following parameters: average size (616.90±38.30 nm; blank: 282.58±22.89 nm), polydispersion index (0.51±0.01; blank: 0.45±0.78), zeta potential (-5.13±0.41 mV; blank: -6.44±1.12 mV) and pH (6.18±0.03; blank: 6.42±0.01). The in vitro biofilm formation assay was performed on dolomite coupons measuring 1 cm2, on which the formulation was applied. There were tested conditions with and without immersion for 72h at 30 ºC. In the tests with the immersed coupons, there was fungal colonization; this was, however, lower than that observed in the control. A. niger decreased by 3 log units. No growth was observed on the coupons that were not immersed. The results were promising and demonstrated viability as a means of antifouling protection, particularly on dry surfaces.

Preliminary insights into the action of non-antibiotic pharmaceuticals in the colonization of Pseudomonas aeruginosa TGC04

“…This study is part of a research project to characterize the strain P. aeruginosa TGC04, a wild type hydrocarbonclastic pseudomonad (29), a high pyocyanin producer (38,39,40), resistant to exposure to different wavelengths of the visible spectrum (41) and capable of using pesticides (42) and polycyclic aromatic hydrocarbons (43) as a carbon source. In addition, the characterization observed here concerns the susceptibility of P. aeruginosa TGC04 surface colonization to non-antibiotic pharmaceuticals.…”

Métodos físicos, químicos e biológicos de controle de biofilmes bacterianos

“…A aplicação industrial de antibióticos na função de biocidas é muito difundida, com destaque para a indústria alimentícia (NG et al, 2020), farmacêutica (COOK e WRIGHT, 2022), assim como de tintas e vernizes (HOQUE et al, 2015;GLADIS et al, 2010). O uso de produtos sintéticos Brazilian Journal of Development, Curitiba, v.8, n.12, p. 78646-78664, dec., 2022 causa sérios problemas ambientais, e o emprego de nanopartículas incorporadas de bioativos naturais pode ser uma opção mais sustentável (SILVA et al, 2022).…”