Antifungal compound, methyl hippurate from Bacillus velezensis CE 100 and its inhibitory effect on growth of Botrytis cinerea

Maung, Chaw Ei Htwe; Lee, Hyung Gwon; Cho, Jeong-Yong; Kim, Kil Yong

doi:10.1007/s11274-021-03046-x

Cited by 18 publications

(9 citation statements)

References 40 publications

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“…Bacillus species are special candidates owing to their extensive suppression of a wide range of phytopathogens. Recently, several studies revealed that B. velezensis is an effective antifungal agent against many pathogenic fungi including Botrytis cinerea , Fusarium verticillioides , F. oxysporum , F. solani , Magnaporthe oryzae, and Ralstonia solanacearum [ 21 , 34 , 36 , 37 , 38 ]. In the present study, the growth of anthracnose causal agents, C. acutatum , C. gloeosporioides , C. dematium , and C. coccodes, was restricted by the antagonistic bacterium, B. velezensis CE 100.…”

Section: Discussionmentioning

confidence: 99%

“…Prapagdee et al [ 43 ] also demonstrated that crude antifungal compounds extracted from culture of B. subtilis SSE4 were highly effective in controlling the growth of C. gloeosporioides, resulting in a reduction in disease severity index of anthracnose on Dendrobium leaves. Moreover, our previous study revealed that mycelial growth of B. cinerea was strongly inhibited by different concentrations of crude extract from chloroform, ethyl acetate, and n-butanol soluble layer of B. velezensis CE 100 culture [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, Bacillus velezensis CE 100 (accession number: KACC 81101BP; Korean Agricultural Culture Collection, KACC) was used and this bacterial strain was isolated from the soil of tomato plant [ 34 ]. Strain CE 100 was sub-cultured onto tryptone soy agar (TSA) medium and a single colony was used to inoculate again in tryptone soy broth (TSB) medium at 30 °C and 120 rpm for 2 days in a shaking incubator.…”

Section: Methodsmentioning

confidence: 99%

“…The plates were incubated at 25 °C for 7–10 days depending on the growth of fungal pathogen. Mycelial growth inhibition was calculated with the following equation, mycelial growth inhibition (%) = R − r/R × 100 (where R is the radius of the fungal colony in the control plate, and r is the radius of the fungal colony in the dual-culture plate) [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…The lipopeptides including iturins, fengycin, and surfactin act as key players in direct antagonism against plant pathogenic bacteria and fungi [ 32 ]. In our previous studies, B. velezensis CE 100 was revealed as an effective agent for controlling phytopathogenic fungi, C. gloeosporioides and Botrytis cinerea through the action of an antifungal tetrapeptide, cyclo-(prolyl-valyl-alanyl-isoleucyl) and methyl hippurate [ 33 , 34 ]. Therefore, the objectives of the present study were to evaluate the efficacy of B. velezensis CE 100 in controlling different Colletotrichum species and to characterize its active antifungal substance involved in the suppression of Colletotrichum species.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Potential of Bacillus velezensis CE 100 for the Control of Different Colletotrichum Species through Isolation of Active Dipeptide, Cyclo-(D-phenylalanyl-D-prolyl)

Kim

Hwang

Noh

et al. 2022

IJMS

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Colletotrichum species are important fungal pathogens causing anthracnose of tropical and subtropical fruit and vegetable crops. Dual culture assay indicated that Bacillus velezensis CE 100 was a strong antagonist against C. acutatum, C. coccodes, C. dematium, and C. gloeosporioides. The volatile organic compounds produced by B. velezensis CE 100 affected mycelial growth of Colletotrichum species tested in our study and caused twisted hyphal structures of all these fungal species. Chloroform crude compounds of B. velezensis CE 100 inhibited four Colletotrichum species in a concentration-dependent manner and induced severe damage in hyphal morphology of these fungal pathogens, including swelling, bulging, and multiple branching. Moreover, the active cyclic dipeptide, cyclo-(D-phenylalanyl-D-prolyl), was isolated from chloroform crude extract and identified by nuclear magnetic resonance (NMR) and mass spectrometry. The inhibitory effect of cyclo-(D-phenylalanyl-D-prolyl) on conidial germination of C. gloeosporioides occurred in a concentration-dependent manner. The conidial germination rate was completely inhibited by a concentration of 3 mg/mL of cyclo-(D-phenylalanyl-D-prolyl). Scanning electron micrographs revealed that the exposure to cyclic dipeptide resulted in seriously deformed hyphae and conidia with shriveled surfaces in dipeptide-treated C. gloeosporioides. Therefore, active dipeptide-producing B. velezensis CE 100 is a promising biocontrol agent for Colletotrichum species causing anthracnose.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antifungal Potential of Bacillus velezensis CE 100 for the Control of Different Colletotrichum Species through Isolation of Active Dipeptide, Cyclo-(D-phenylalanyl-D-prolyl)

Kim

Hwang

Noh

et al. 2022

IJMS

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Enhancing tomato disease resistance through endogenous antifungal proteins and introduced nematode‐targeting dsRNA of biocontrol agent Bacillus velezensisHS‐3

Han,

Zhu,

Liu

et al. 2024

Pest Management Science

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BACKGROUNDAs a type of biological control agents (BCAs), Bacillus velezensis possesses the efficacy of inhibiting pathogenic microorganisms, promoting plant growth, and overcoming continuous cropping obstacles (CCOs). However, there is limited reporting on the optimization of the cultivation conditions for such biocontrol agents and their role as dsRNA delivery vectors.RESULTSIn this study, a B. velezensis strain HS‐3 was isolated from the root zone of tomato plants with in vitro anti‐Botrytis cinerea activity. The investigation into active compounds revealed that HS‐3 predominantly employs proteins with molecular weights greater than 3 kDa for its antifungal activity. LC–MS/MS analysis identified various proteases and chitosanase, further suggesting that HS‐3 most likely employs these enzymes to degrade fungal cell walls for its antifungal effect. To optimize the production of extracellular proteins, fermentation parameters for HS‐3 were systematically optimized, leading to an optimized medium (OP‐M). HS‐3 cultured in OP‐M demonstrated enhanced capacity to assist tomato plants in withstanding CCO. However, the presence of excessive nematodes in diseased soil resulted in disease severity index (DSI) remaining high. An RNA interference mechanism was further introduced to HS‐3, targeting the nematode tyrosine phosphatase (TP) gene. Ultimately, HS‐3 expressing dsRNA of TP in OP‐M effectively assisted tomatoes in mitigating CCO, reducing DSI to 2.2% and 17.8% of the control after 45 and 90 days of growth, respectively.CONCLUSIONThe advantages of Bacillus velezensis in crop disease management and the mitigation of CCOs become even more pronounced when utilizing both optimized levels of endogenous enzymes and introduced nematode‐targeting dsRNA.

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Exploring the Effects of Methyl Jasmonate and Lipopeptides against Gray Mold in Strawberry fruit (Fragaria x ananassa Duch.)

Malik,

AL-Huqail,

Alghanem

et al. 2024

J Plant Growth Regul

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Antifungal compound, methyl hippurate from Bacillus velezensis CE 100 and its inhibitory effect on growth of Botrytis cinerea

Cited by 18 publications

References 40 publications

Antifungal Potential of Bacillus velezensis CE 100 for the Control of Different Colletotrichum Species through Isolation of Active Dipeptide, Cyclo-(D-phenylalanyl-D-prolyl)

Antifungal Potential of Bacillus velezensis CE 100 for the Control of Different Colletotrichum Species through Isolation of Active Dipeptide, Cyclo-(D-phenylalanyl-D-prolyl)

Enhancing tomato disease resistance through endogenous antifungal proteins and introduced nematode‐targeting dsRNA of biocontrol agent Bacillus velezensisHS‐3

Exploring the Effects of Methyl Jasmonate and Lipopeptides against Gray Mold in Strawberry fruit (Fragaria x ananassa Duch.)

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