Antifungal curcumin promotes chitin accumulation associated with decreased virulence of Sporothrix schenckii

Huang, Lilin; Zhang, Jing; Song, Tian-Zhang; Yuan, Long; Zhou, Junjie; Yin, Hongling; He, Tailong; Gao, Wenchao; Sun, Yao; Hu, Xuchu; Huang, Huaiqiu

doi:10.1016/j.intimp.2016.03.010

Cited by 28 publications

(14 citation statements)

References 27 publications

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“…growth (53). Curcumin is also active against Sporothrix schenckii, the cause of sporotrichosis, redistributing chitin in its cell wall (38). The activity of this compound can be modified in combination with other molecules, such as dodecanol or ascorbic acid (42,95).…”

Section: Polyphenolsmentioning

confidence: 99%

Plant Phytochemicals in Food Preservation: Antifungal Bioactivity: A Review

Redondo-Blanco

Fernández

López-Ibáñez

et al. 2020

Journal of Food Protection

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Synthetic food additives generate a negative perception in consumers. This fact generates an important pressure on food manufacturers, searching for safer natural alternatives. Phytochemicals (such as polyphenols and thiols) and plant essential oils (terpenoids) possess antimicrobial activities that are able to prevent food spoilage due to fungi (e.g., Aspergillus, Penicillium) and intoxications (due to mycotoxins), both of which are important economic and health problems worldwide. This review summarizes industrially interesting antifungal bioactivities from the three main types of plant nutraceuticals: terpenoids (as thymol), polyphenols (as resveratrol) and thiols (as allicin) as well as some of the mechanisms of action. These phytochemicals are widely distributed in fruits and vegetables and are very useful in food preservation as they inhibit growth of important spoilage and pathogenic fungi, affecting especially mycelial growth and germination. Terpenoids and essential oils are the most abundant group of secondary metabolites found in plant extracts, especially in common aromatic plants, but polyphenols are a more remarkable group of bioactive compounds as they show a broad array of bioactivities. HIGHLIGHTS

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Section: Polyphenolsmentioning

confidence: 99%

Plant Phytochemicals in Food Preservation: Antifungal Bioactivity: A Review

Redondo-Blanco

Fernández

López-Ibáñez

et al. 2020

Journal of Food Protection

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“…This suggests that cell wall integrity was altered, leading to the exposure of chitin, the main structural polysaccharide of internal wall. Namely, the fluorescence increment might be explained by the modification or loss in the outermost cell surface proteins, which expose internal cell wall components (Kubitschek-Barreira et al, 2013; Huang et al, 2016). Moreover, when conidia treated with harmol were observed by TEM, the most evident effect was wall distortion, with the consequent leak of cytoplasmic content at least in B. cinerea .…”

Section: Discussionmentioning

confidence: 99%

UVA Photoactivation of Harmol Enhances Its Antifungal Activity against the Phytopathogens Penicillium digitatum and Botrytis cinerea

et al. 2017

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Phytopathogenic fungi responsible for post-harvest diseases on fruit and vegetables cause important economic losses. We have previously reported that harmol (1-methyl-9H-pyrido[3,4-b]indol-7-ol) is active against the causal agents of green and gray molds Penicillium digitatum and Botrytis cinerea, respectively. Here, antifungal activity of harmol was characterized in terms of pH dependency and conidial targets; also photodynamic effects of UVA irradiation on the antimicrobial action were evaluated. Harmol was able to inhibit the growth of both post-harvest fungal disease agents only in acidic conditions (pH 5), when it was found in its protonated form. Conidia treated with harmol exhibited membrane integrity loss, cell wall disruption, and cytoplasm disorganization. All these deleterious effects were more evident for B. cinerea in comparison to P. digitatum. When conidial suspensions were irradiated with UVA in the presence of harmol, antimicrobial activity against both pathogens was enhanced, compared to non-irradiated conditions. B. cinerea exhibited a high intracellular production of reactive oxygen species (ROS) when was incubated with harmol in irradiated and non-irradiated treatments. P. digitatum showed a significant increase in ROS accumulation only when treated with photoexcited harmol. The present work contributes to unravel the antifungal activity of harmol and its photoexcited counterpart against phytopathogenic conidia, focusing on ROS accumulation which could account for damage on different cellular targets.

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“…Este resultado sugere que a CUR possivelmente regulou a síntese de quitina através da via de sinalização PKC, não sendo possível confirmar se o mecanismo da CUR é exatamente a acumulação, mas ofereceu evidências que indicam essa relação. A acumulação e a exposição de quitina, descrito no estudo, potencializou a depuração dos fungos e a diminuição dos fungos em camundongos, sugerindo uma baixa na virulência de S. schenckii causada pela CUR (Huang et al, 2016). Dessa forma, a perturbação da quitina e da sua biossíntese mostrou que ainda é uma área terapêutica promissora a ser explorada e vêm exibindo ótimos resultados.…”

Section: Parede Celularunclassified

“…A CUR foi citada em duas publicações que avaliavam sua ação sobre regiões distintas, porém ambas foram positivas (MP e parede celular). A dualidade pode ser de extrema serventia caso este composto venha a evoluir nas pesquisas e se torne um agente antifúngico utilizado clinicamente, entretanto mais estudos são necessários para esclarecer esta possibilidade (Khan et al, 2012;Huang et al, 2016). Tanto a MP quanto a parede celular caracterizam envoltórios importantes para a célula fúngica e possuem importantes pontos de interesse terapêutico, a primeira tem suas funções voltadas ao controle químico entre o meio intracelular e extracelular, já a segunda está principalmente ligada a proteção física, patogenicidade e reconhecimento celular.…”

Section: Parede Celularunclassified