Antifungal efficacy of pyocyanin produced from bioindicators of nosocomial hazards

Sudhakar, Tata; Karpagam, S.

doi:10.1109/gtec.2011.6167673

Cited by 8 publications

(10 citation statements)

References 8 publications

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“…While in two additional studies, the MIC values ranged between 40 and 128 μg/ml against Candida spp. and Cryptococcus neoformans and proved to be 64 μg/ml against A. fumigatus and A. flavus isolates ( Sudhakar and Karpagam, 2011 ; Sudhakar et al, 2013 ). In the present study, we focused on the previously measured peak levels of pyocyanin in the sputum ( Wilson et al, 1988 ), which are generally lower than the MICs determined by previous reports.…”

Section: Discussionmentioning

confidence: 99%

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

et al. 2019

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Members of the Scedosporium apiospermum species complex are the second most frequently isolated pathogens after Aspergillus fumigatus from cystic fibrosis (CF) patients with fungal pulmonary infections. Even so, the main risk factors for the infection are unrevealed. According to previous studies, bacterial infections might reduce the risk of a fungal infection, but an antibacterial therapy may contribute to the airway colonization by several fungal pathogens. Furthermore, corticosteroids, which are often used to reduce lung inflammation in children and adults with CF, are also proved to enhance the growth of A. fumigatus in vitro. Considering all the above discussed points, we aimed to test how Pseudomonas aeruginosa influences the growth of scedosporia and to investigate the potential effect of commonly applied antibacterial agents and corticosteroids on Scedosporium species. Direct interactions between fungal and bacterial strains were tested using the disk inhibition method. Indirect interactions via volatile compounds were investigated by the plate-in-plate method, while the effect of bacterial media-soluble molecules was tested using a modified cellophane assay and also in liquid culture media conditioned by P. aeruginosa. To test the effect of bacterial signal molecules, antibacterial agents and corticosteroids on the fungal growth, the broth microdilution method was used. We also investigated the germination ability of Scedosporium conidia in the presence of pyocyanin and diffusible signal factor by microscopy. According to our results, P. aeruginosa either inhibited or enhanced the growth of scedosporia depending on the culture conditions and the mode of interactions. When the two pathogens were cultured physically separately from each other in the plate-in-plate tests, the presence of the bacteria was able to stimulate the growth of several fungal isolates. While in direct physical contact, bacterial strains inhibited the fungal growth. This effect might be attributed to bacterial signal molecules, which also proved to inhibit the germination and growth of scedosporia. In addition, antibacterial agents showed growth-promoting, while corticosteroids exhibited growth inhibitory effect on several Scedosporium isolates. These data raise the possibility that a P. aeruginosa infection or a previously administered antibacterial therapy might be able to increase the chance of a Scedosporium colonization in a CF lung.

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Section: Discussionmentioning

confidence: 99%

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

et al. 2019

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“…S5 ) was employed together with the β-galactosidase gene from E. coli as a reporter. Both the main end product pyocyanin and other pathway-related phenazines including phenazine-1-carboxylic acid, or short PCA, display antibacterial and antifungal properties [44] , [45] , [46] . The genes phzA-G , spanning 6.4 kb and encoding PCA biosynthesis, as well as the promoter-less β-galactosidase encoding lacZ gene (3.1 kb) were PCR amplified.…”

Section: Resultsmentioning

confidence: 99%

Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX

Domröse

Weihmann

Thies

et al. 2017

Synthetic and Systems Biotechnology

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Microbial secondary metabolites represent a rich source of valuable compounds with a variety of applications in medicine or agriculture. Effective exploitation of this wealth of chemicals requires the functional expression of the respective biosynthetic genes in amenable heterologous hosts. We have previously established the TREX system which facilitates the transfer, integration and expression of biosynthetic gene clusters in various bacterial hosts. Here, we describe the yTREX system, a new tool adapted for one-step yeast recombinational cloning of gene clusters. We show that with yTREX, Pseudomonas putida secondary metabolite production strains can rapidly be constructed by random targeting of chromosomal promoters by Tn5 transposition. Feasibility of this approach was corroborated by prodigiosin production after yTREX cloning, transfer and expression of the respective biosynthesis genes from Serratia marcescens. Furthermore, the applicability of the system for effective pathway rerouting by gene cluster adaptation was demonstrated using the violacein biosynthesis gene cluster from Chromobacterium violaceum, producing pathway metabolites violacein, deoxyviolacein, prodeoxyviolacein, and deoxychromoviridans. Clones producing both prodigiosin and violaceins could be readily identified among clones obtained after random chromosomal integration by their strong color-phenotype. Finally, the addition of a promoter-less reporter gene enabled facile detection also of phenazine-producing clones after transfer of the respective phenazine-1-carboxylic acid biosynthesis genes from Pseudomonas aeruginosa. All compounds accumulated to substantial titers in the mg range. We thus corroborate here the suitability of P. putida for the biosynthesis of diverse natural products, and demonstrate that the yTREX system effectively enables the rapid generation of secondary metabolite producing bacteria by activation of heterologous gene clusters, applicable for natural compound discovery and combinatorial biosynthesis.

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“…Also, Rahman et al (2009) found that pyocyanin extracted from P. aeruginosa DSO-19 has antibacterial activity against S. aureus, Staphylococcus epidermis, Bacillus subtilis and Micrococcus luteus. Moreover, Sudhakar et al (2013) found that pigment isolated from P. aeruginosa SU1 has the maximum On the side of pyocyanin's antifungal activity, it was found that pyocyanin isolated from several P. aeruginosa species has antifungal activity against Aspergillus and Candida spp., i.e. Aspergillus fumigatus, A. niger and C. albicans (Kerr 1994;Kerr et al 1999).…”

Section: Antimicrobial Activity Of Pyocyanin Isolated From P Aeruginosa As6mentioning

confidence: 99%

“…There was clinical evidence that pyocyanin suppressed the growth of different C. albicans in patients with lung infection (Pal and Revathi 1998;Kaleli et al 2007), and other diseases (Marrez and Haitham 2020). Sudhakar et al (2013) reported that pyocyanin separated from P. aeruginosa WS1 encountered phytopathogens with MIC value of 64 lg mL À1 against A. flavus and Aspergillus fumigates, and 128 lg mL À1 against Candida species.…”

Section: Antimicrobial Activity Of Pyocyanin Isolated From P Aeruginosa As6mentioning

confidence: 99%

“…However, others demonstrated that phenazine antibiotics' activity is concentration dependence; therefore, when the concentration of phenazine increased, the biological activity would be increased (Stephen 1981). Sudhakar et al (2013) also stated that pyocyanin isolated from P. aeruginosa SU1 has the highest antibacterial activity against E. coli.…”

Section: Feed Samplesmentioning

confidence: 99%

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Impacts of dietary supplementation of pyocyanin powder on growth performance, carcase traits, blood chemistry, meat quality and gut microbial activity of broilers

Ashour

Farsi

Alaidaroos

et al. 2021

Italian Journal of Animal Science

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Natural antioxidants play an important role in maintaining and improving poultry's well-being, survival and productive and reproductive performance. Pyocyanin, a secondary blue redox-active metabolite, is one of these natural antioxidants that exert several biological activities to improve birds' performance. This study estimated the effect of dietary supplementation with pyocyanin powder (PP) on broiler's growth, carcase and serum properties, meat quality and microbial load activity. A total of 180 1-week-old unsexed chicks were randomly allocated into three groups. The first group served as a control (C) and fed the basal diet, while the second and third groups (P75 and P150) were fed the basal diet supplemented with 75.0 and 150.0 mg PP/kg, respectively. Results showed that overall body weight gain (BWG) was improved (p<.05) in P75 compared to P150 and control groups. Feed intake and feed conversion ratio (FCR) of chicks fed the diet containing PP levels were not altered during all experimental periods compared to the control. Dietary treatments did not affect all carcase traits, blood biochemical parameters and meat sensory characteristics at five weeks of age. Antioxidative status was improved by pyocyanin supplementation as serum malondialdehyde (MDA) was reduced while GST and GSH levels were elevated in P75 and P150 compared to the control. Dietary PP supplements increased (p<.05) the pH, lightness and redness of pectoralis major muscle and reduced (p<.05) the yellowness of the muscle and its contents of thiobarbituric acid and total volatile basic nitrogen. The PP addition showed antibacterial and antifungal activities against several pathogenic bacteria and mycotoxigenic fungi. It could be concluded that pyocyanin can be introduced as a natural, functional and phytogenic feed additive to boost broiler's growth, improve their meat quality, produce nutritious meat products and reduce pathogenic bacteria without adverse impacts on their productivity. HIGHLIGHTSThis work investigated the effect of pyocyanin powder on broiler chickens. The addition of pyocyanin at 75.0 mg/kg diet improved Body weight gain (BWG) and meat quality characteristics. Pyocyanin exerted antibacterial and antifungal activity against several pathogenic bacteria. Pyocyanin could be used as a natural and functional additive to poultry diets.

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Antifungal efficacy of pyocyanin produced from bioindicators of nosocomial hazards

Cited by 8 publications

References 8 publications

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

In vitro Interactions of Pseudomonas aeruginosa With Scedosporium Species Frequently Associated With Cystic Fibrosis

Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX

Impacts of dietary supplementation of pyocyanin powder on growth performance, carcase traits, blood chemistry, meat quality and gut microbial activity of broilers

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