Antifungal Halogenated Cyclopentenones from the Endophytic Fungus <i>Saccharicola bicolor</i> of <i>Bergenia purpurascens</i> by the One Strain-Many Compounds Strategy

Zhao, Min; Guo, Da‐Le; Liu, Guanghui; Fu, Xianpeng; Gu, Yu‐Cheng; Ding, Li‐Sheng; Zhou, Yan

doi:10.1021/acs.jafc.9b06594

Cited by 20 publications

(18 citation statements)

References 32 publications

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“…MAPKs are a family of serine/threonine protein kinases that mediate fundamental biological processes, signal transduction and cell responses and phosphorylate various substrate proteins, including proinflammatory transcription factors; thus, MAPKs are potential targets for anti-inflammatory therapeutics ( Liang et al, 2014 ). In mammals, The MAPK family includes the extracellular signal-regulated kinase (ERK), Jun N-terminal kinase (JNK) and p38 pathways ( Zhao et al, 2020 ). Together with the findings obtained in this study, we found that after Kp infection, Bm could regulate the MAPK pathway by inactivating JNK, ERK and p38.…”

Section: Discussionmentioning

confidence: 99%

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

et al. 2021

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Background:Klebsiella pneumonia has emerged as a critical pathogen causing severe clinical problems, such as pneumonia and sepsis. Meanwhile, intensified drug resistance induced by antibiotic therapy necessitates discovering novel and active molecules from Traditional Chinese Medicine (TCM) for treatment.Methods and results: In this study, the isolated Bergenin monohydrate showed an anti-inflammatory effect in Klebsiella-infected mice. We initially investigated the anti-inflammatory effects and cytoprotection against oxidative stress in vitro and in vivo. Interestingly, a specific dose of Bm can effectively ameliorate lung injury and suppress the expression of inflammatory cytokines such as TNF-α, IL-6, IL-1β and PEG2. Moreover, Bm was also shown to reduced the levels of MPO, MDA and increased SOD and GSH activities. Moreover, we assessed the intracellular signaling molecules including p38, ERK, JNK, IκB, NF-κB-p65 by western blotting and verified through MAPK and NF-κB pathways inhibition experiments. These results reveal that Bm executed its effects via the classical MAPK signaling pathway and NF-κB pathway.Conclusion: Given its underlying anti-inflammatory effect, Bm may be used as a promising therapeutic against Klebsiella-induced infection, thus providing a benefit for the future clinical therapy of pneumonia and medicine design.

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Section: Discussionmentioning

confidence: 99%

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

et al. 2021

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“…Plant diseases caused by microorganisms lead to significant crop losses worldwide ( Oerke, 2006 ; Cantrell et al, 2012 ; Tan et al, 2018 ; Sang and Kim, 2020 ). It is estimated that the fungal diseases cause approximately 20% of yield reductions in commercial crops and major foods worldwide ( Wang et al, 2016 ; Fisher et al, 2018 ; Zhao et al, 2020 ). In Brazil, economically important crops such as citrus and maize are affected by different phytopathogens that can cause losses in production or render products unviable for export ( Ferrigo et al, 2016 ; Guarnaccia et al, 2017 ; Ference et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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Plant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi of leaves and petioles of medicinal plants Vochysia divergens and Stryphnodendron adstringens located in two regions of high diversity in Brazil, Pantanal, and Cerrado, respectively. We recovered 1,304 fungal isolates and based on the characteristics of the culture, were assigned to 159 phenotypes. One isolate was selected as representative of each phenotype and studied for antimicrobial activity against phytopathogens. Isolates with better biological activities were identified based on DNA sequences and phylogenetic analyzes. Among the 159 representative isolates, extracts from 12 endophytes that inhibited the mycelial growth (IG) of Colletotrichum abscissum (≥40%) were selected to expand the antimicrobial analysis. The minimum inhibitory concentrations (MIC) of the extracts were determined against citrus pathogens, C. abscissum, Phyllosticta citricarpa and Xanthomonas citri subsp. citri and the maize pathogen Fusarium graminearum. The highest activity against C. abscissum were from extracts of Pseudofusicoccum stromaticum CMRP4328 (IG: 83% and MIC: 40 μg/mL) and Diaporthe vochysiae CMRP4322 (IG: 75% and MIC: 1 μg/mL), both extracts also inhibited the development of post-bloom fruit drop symptoms in citrus flowers. The extracts were promising in inhibiting the mycelial growth of P. citricarpa and reducing the production of pycnidia in citrus leaves. Among the isolates that showed activity, the genus Diaporthe was the most common, including the new species D. cerradensis described in this study. In addition, high performance liquid chromatography, UV detection, and mass spectrometry and thin layer chromatography analyzes of extracts produced by endophytes that showed high activity, indicated D. vochysiae CMRP4322 and P. stromaticum CMRP4328 as promising strains that produce new bioactive natural products. We report here the capacity of endophytic fungi of medicinal plants to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria. The description of the new species D. cerradensis, reinforces the ability of medicinal plants found in Brazil to host a diverse group of fungi with biotechnological potential.

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“…In antifungal assay, 10 μg/μL concentration of 115 showed inhibition of Bipolaris Sorokiniana (57.14%), A. flavus (52.5%), and A. alternata (91.25%) as compared to control ( Goutam et al, 2017 ). Six new halogenated cyclopentenones, including four chlorinated, bicolorins A (116), B (117), D (118), and E (119), and two brominated bicolorins G (120) and H (121), were isolated from the endophytic fungus Saccharicola bicolor of Bergenia purpurascens ( Zhao et al, 2020 ). Compounds 116-121 possessed weak to moderate activity against five pathogenic fungi- Uromyces viciae-fabae, Pythium dissimile, G. zeae, A. niger , and S. sclerotiorum , with MICs of 26.8-380.9 μM.…”

Section: Chemical Diversity Of Antifungal Secondary Metabolites From Endophytic Fungimentioning

confidence: 99%

“…117 and 118, in particular, exhibited moderate activity against P. dissimile with the MICs of 33.0 and 44.7 μM, respectively, compared with the positive control cycloheximide (MIC 30.6 μM). Additionally, 118 was proven to be potently antifungal against S. sclerotiorum in vivo , indicating its potential as a candidate of new natural fungicides ( Zhao et al, 2020 ). A new dibenzo-α-pyrone rhizopycnin D (122) and a known congener TMC-264 (123) were isolated from the endophytic fungus Rhizopycnis vagum Nitaf22 obtained from Nicotiana tabacum ( Lai et al, 2016 ).…”

Section: Chemical Diversity Of Antifungal Secondary Metabolites From Endophytic Fungimentioning

confidence: 99%

Antifungal Secondary Metabolites Produced by the Fungal Endophytes: Chemical Diversity and Potential Use in the Development of Biopesticides

Zhao

et al. 2021

Front. Microbiol.

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Plant diseases caused by phytopathogenic fungi can lead to huge losses in the agricultural fields and therefore remain a continuous threat to the global food security. Chemical-based fungicides contributed significantly in securing crop production. However, indiscriminate application of fungicides has led to increased chemical resistance and potential risks to human health and environment. Thus, there is an urgent need for searching for new bioactive natural products and developing them into new biopesticides. Fungal endophytes, microorganisms that reside in the fresh tissues of living plants, are regarded as untapped sources of novel natural products for exploitation in agriculture and/or medicine. Chemical examination of endophytic fungi has yielded enormous antifungal natural products with potential use in the development of biopesticides. This review summarizes a total of 132 antifungal metabolites isolated from fungal endophytes in the past two decades. The emphasis is on the unique chemical diversity of these metabolic products, together with their relevant antifungal properties. Moreover, some “star molecules,” such as griseofulvin and trichothecene, as well as their synthetic derivatives that possess high potential as candidates of new natural fungicides, are also presented herein.

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Antifungal Halogenated Cyclopentenones from the Endophytic Fungus Saccharicola bicolor of Bergenia purpurascens by the One Strain-Many Compounds Strategy

Cited by 20 publications

References 32 publications

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Antifungal Secondary Metabolites Produced by the Fungal Endophytes: Chemical Diversity and Potential Use in the Development of Biopesticides

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