Antifungal mechanisms of silver nanoparticles on mycotoxin producing rice false smut fungus

Wen, Hui; Shi, Huanbin; Jiang, Nan; Qiu, Jian; Lin, Fu‐Cheng; Kou, Yanjun

doi:10.1016/j.isci.2022.105763

Cited by 28 publications

(10 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the mycelia were collected and fixed with 2.5% (v/v) glutaraldehyde solution, stained with 1% (w/v) osmium tetroxide and dehydrated with a series of ethanol solutions (30-100%) as described. 37 The morphology of mycelia and biosynthesized AgNPs was captured with SEM (SU8010, Hitachi, Japan) and TEM (H7650, JEOL, Japan).…”

Section: Scanning Electron Microscopy (Sem) and Transmission Electron...mentioning

confidence: 99%

Antifungal activity and mechanisms of AgNPs and their combination with azoxystrobin against Magnaporthe oryzae

Shi¹,

Wen²,

Xie³

et al. 2023

Environ. Sci.: Nano

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Scanning Electron Microscopy (Sem) and Transmission Electron...mentioning

confidence: 99%

Antifungal activity and mechanisms of AgNPs and their combination with azoxystrobin against Magnaporthe oryzae

Shi¹,

Wen²,

Xie³

et al. 2023

Environ. Sci.: Nano

Self Cite

View full text Add to dashboard Cite

show abstract

“…With regard to ustilaginoidins, the ustilaginoidin synthesis (Ugs) gene cluster has been identified to be responsible for their biosynthesis . As reported, silver nanoparticles could upregulate several Ugs genes, resulting in an increase of ustilaginoidin production . Herein, the transcriptome data revealed that 2,4-DTBP treatment resulted in 2.63-fold downregulation of UvPKS1 (UV8b_02086), which encodes the major polyketide synthase catalyzing the first step of ustilaginoidin biosynthesis .…”

Section: Resultsmentioning

confidence: 79%

“…2 As reported, silver nanoparticles could upregulate several Ugs genes, resulting in an increase of ustilaginoidin production. 49 Herein, the transcriptome data revealed that 2,4-DTBP treatment resulted in 2.63-fold downregulation of UvPKS1 (UV8b_02086), which encodes the major polyketide synthase catalyzing the first step of ustilaginoidin biosynthesis. 2 Thus, the downregulation of UvPKS1 suggested that 2,4-DTBP might inhibit the production of ustilaginoidin in U. virens, presenting more advantages and potential applications in pathogenic control.…”

Section: ■ Materials and Methodsmentioning

confidence: 90%

Antifungal Activity and Action Mechanisms of 2,4-Di-tert-butylphenol against Ustilaginoidea virens

Fan,

Yu,

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Ustilaginoidea virens is a destructive phytopathogenic fungus that causes false smut disease in rice. In this study, the natural product 2,4-di-tert-butylphenol (2,4-DTBP) was found to be an environmentally friendly and effective agent for the first time, which exhibited strong antifungal activity against U. virens, with an EC50 value of 0.087 mmol/L. The scanning electron microscopy, fluorescence staining, and biochemical assays indicated that 2,4-DTBP could destroy the cell wall, cell membrane, and cellular redox homeostasis of U. virens, ultimately resulting in fungal cell death. Through the transcriptomic analysis, a total of 353 genes were significantly upregulated and 367 genes were significantly downregulated, focusing on the spindle microtubule assembly, cell wall and membrane, redox homeostasis, mycotoxin biosynthesis, and intracellular metabolism. These results enhanced the understanding of the antifungal activity and action mechanisms of 2,4-DTBP against U. virens, supporting it to be a potential antifungal agent for the control of false smut disease.

show abstract

“…Metals such as Ag, Cu, Fe, Zn, Se, Ni, Au, Zr, Ce, Ti, and Pd have been studied in regard to compounds possessing antifungal activity [9][10][11][12]. At the same time, current scientific research on the antifungal properties of metals is mainly focused on the study of Ag and Au nanoparticles (NPs) [10][11][12][13][14][15] since the antimicrobial effectiveness of their action has been known for a long time.…”

Section: Introductionmentioning

confidence: 99%

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Lyagin

Aslanli

Domnin

et al. 2023

IJMS

View full text Add to dashboard Cite

Active research of metal-containing compounds and enzymes as effective antifungal agents is currently being conducted due to the growing antifungal resistance problem. Metals are attracting special attention due to the wide variety of ligands that can be used for them, including chemically synthesized and naturally obtained variants as a result of the so-called “green synthesis”. The main mechanism of the antifungal action of metals is the triggering of the generation and accumulation of reactive oxygen species (ROS). Further action of ROS on various biomolecules is nonspecific. Various hydrolytic enzymes (glucanases and proteases), in turn, exhibit antifungal properties by affecting the structural elements of fungal cells (cell walls, membranes), fungal quorum sensing molecules, fungal own protective agents (mycotoxins and antibiotics), and proteins responsible for the adhesion and formation of stable, highly concentrated populations in the form of biofilms. A wide substrate range of enzymes allows the use of various mechanisms of their antifungal actions. In this review, we discuss the prospects of combining two different types of antifungal agents (metals and enzymes) against mycelial fungi and yeast cells. Special attention is paid to the possible influence of metals on the activity of the enzymes and the possible effects of proteins on the antifungal activity of metal-containing compounds.

show abstract

Antifungal mechanisms of silver nanoparticles on mycotoxin producing rice false smut fungus

Cited by 28 publications

References 54 publications

Antifungal activity and mechanisms of AgNPs and their combination with azoxystrobin against Magnaporthe oryzae

Antifungal activity and mechanisms of AgNPs and their combination with azoxystrobin against Magnaporthe oryzae

Antifungal Activity and Action Mechanisms of 2,4-Di-tert-butylphenol against Ustilaginoidea virens

Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

Contact Info

Product

Resources

About