Antifungal mechanisms of γ-aminobutyric acid against the postharvest pathogen Alternaria alternata

Yu, Sainan; Zhen, Chaoying; Zhao, Pengyu; Li, Junjie; Qin, Zhen; Gao, Haiyan

doi:10.1016/j.lwt.2022.114314

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…While the precise biochemical, physiological, and molecular mechanisms underlying the antifungal activity of GABA and ß -alanine remain poorly investigated, numerous potential modes of action have been proposed. GABA may interfere with fungal cell wall synthesis and disrupt cellular signaling pathways ( Yu et al., 2023 ). For example, GABA inhibited the mycelial growth of Alternaria alternata , the causal agent of black spot disease in tomatoes, via downregulating several genes related to sporulation, toxin synthesis, and cell wall degradation enzymes ( Yu et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

“…GABA may interfere with fungal cell wall synthesis and disrupt cellular signaling pathways ( Yu et al., 2023 ). For example, GABA inhibited the mycelial growth of Alternaria alternata , the causal agent of black spot disease in tomatoes, via downregulating several genes related to sporulation, toxin synthesis, and cell wall degradation enzymes ( Yu et al., 2023 ). Likewise, BABA significantly inhibited mycelial growth, spore germination, and germ tube elongation of P. digitatum , the causal agent of green mold in orange fruit, via increasing the relative electrical conductivity, as well as malondialdehyde (MDA) levels in the mycelium, but caused a considerable reduction in the ergosterol content in the plasma membrane and the total lipid content of the mycelium ( Elsherbiny et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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Non-proteinogenic amino acids mitigate oxidative stress and enhance the resistance of common bean plants against Sclerotinia sclerotiorum

Nehela,

Mazrou,

El_Gammal

et al. 2024

Front. Plant Sci.

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White mold, caused by the necrotrophic fungus Sclerotinia sclerotiorum, is a challenging disease to common bean cultivation worldwide. In the current study, two non-proteinogenic amino acids (NPAAs), γ-aminobutyric acid (GABA) and ß-alanine, were suggested as innovative environmentally acceptable alternatives for more sustainable management of white mold disease. In vitro, GABA and ß-alanine individually demonstrated potent dose-dependent fungistatic activity and effectively impeded the radial growth and development of S. sclerotiorum mycelium. Moreover, the application of GABA or ß-alanine as a seed treatment followed by three root drench applications efficiently decreased the disease severity, stimulated plant growth, and boosted the content of photosynthetic pigments of treated S. sclerotiorum-infected plants. Furthermore, although higher levels of hydrogen peroxide (H2O2), superoxide anion (O2•−), and malondialdehyde (MDA) indicated that S. sclerotiorum infection had markedly triggered oxidative stress in infected bean plants, the exogenous application of both NPAAs significantly reduced the levels of the three studied oxidative stress indicators. Additionally, the application of GABA and ß-alanine increased the levels of both non-enzymatic (total soluble phenolics and flavonoids), as well as enzymatic (catalase [CAT], peroxidases [POX], and polyphenol oxidase [PPO]) antioxidants in the leaves of S. sclerotiorum-infected plants and improved their scavenging activity and antioxidant efficiency. Applications of GABA and ß-alanine also raised the proline and total amino acid content of infected bean plants. Lastly, the application of both NPAAs upregulated the three antioxidant-related genes PvCAT1, PvCuZnSOD1, and PvGR. Collectively, the fungistatic activity of NPAAs, coupled with their ability to alleviate oxidative stress, enhance antioxidant defenses, and stimulate plant growth, establishes them as promising eco-friendly alternatives for white mold disease management for sustainable bean production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Non-proteinogenic amino acids mitigate oxidative stress and enhance the resistance of common bean plants against Sclerotinia sclerotiorum

Nehela,

Mazrou,

El_Gammal

et al. 2024

Front. Plant Sci.

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“…Several species of the Alternaria genus are well-known as phytopathogens, which infect many genera of vegetables and fruits. These species have a saprophytic nature, and their pathogenicity actions were due to toxins and cell-wall degrading enzymes [ 57 ]. Among those are Alternaria dauci, A. solani, A. tenuis, A. brassicae , A. brassicicola , and A. alternata .…”

Section: Discussionmentioning

confidence: 99%

“…A. alternata is also considered one of the most important diseases affecting common bean plants under the temperate conditions, as a serious foliar ailment causing massive yield reductions [ 11 , 26 ]. In addition, other crops have been infected, such as faba bean Vicia faba L. [ 15 ], tomato [ 57 ], apple [ 12 ], and orange Citrus spp. [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Nanofungicides with Selenium and Silicon Can Boost the Growth and Yield of Common Bean (Phaseolus vulgaris L.) and Control Alternaria Leaf Spot Disease

et al. 2023

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There is an urgent need to reduce the intensive use of chemical fungicides due to their potential damage to human health and the environment. The current study investigated whether nano-selenium (nano-Se) and nano-silica (nano-SiO2) could be used against the leaf spot disease caused by Alternaria alternata in a common bean (Phaseolus vulgaris L.). The engineered Se and SiO2 nanoparticles were compared to a traditional fungicide and a negative control with no treatment, and experiments were repeated during two successive seasons in fields and in vitro. The in vitro study showed that 100 ppm nano-Se had an efficacy rate of 85.1% on A. alternata mycelial growth, followed by the combined applications (Se + SiO2 at half doses) with an efficacy rate of 77.8%. The field study showed that nano-Se and the combined application of nano-Se and nano-SiO2 significantly decreased the disease severity of A. alternata. There were no significant differences among nano-Se, the combined application, and the fungicide treatment (positive control). As compared to the negative control (no treatment), leaf weight increased by 38.3%, the number of leaves per plant by 25.7%, chlorophyll A by 24%, chlorophyll B by 17.5%, and total dry seed yield by 30%. In addition, nano-Se significantly increased the enzymatic capacity (i.e., CAT, POX, PPO) and antioxidant activity in the leaves. Our current study is the first to report that the selected nano-minerals are real alternatives to chemical fungicides for controlling A. alternata in common beans. This work suggests the potential of nanoparticles as alternatives to fungicides. Further studies are needed to better understand the mechanisms and how different nano-materials could be used against phytopathogens.

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“…In the case of Alternaria rot disease, the exogenous application of GABA on tomato plants led to the development of a resistance phenotype against this fungus (Yang et al, 2017). Additionally, a recent study has revealed that GABA also provides resistance against Alternaria alternata on fruits and vegetables during their post-harvest storage by decreasing their sporulation and downregulating the toxin synthesis (Yu et al, 2023). GABA also induced the activities of antioxidant enzymes, including CAT, SOD, and POD, to promote the detoxification of stress-induced ROS.…”

Section: Role Of Gaba In Alleviating the Fungal Pathogen Attackmentioning

confidence: 99%

GABA in plants: developmental and stress resilience perspective

Gahlowt,

Tripathi,

Singh

et al. 2023

Physiologia Plantarum

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Gamma‐aminobutyric acid (GABA), a ubiquitously present non‐proteinogenic amino acid, has recently emerged as a key regulator of growth and development in plants during normal as well as challenging environmental conditions. GABA biosynthesis has been reported at multiple stages of plant development, particularly during vegetative and reproductive stages and in response to stress conditions. Accumulating evidence has highlighted the crucial roles of various cell cycle regulators such as type‐D cyclins and CDK;A1, transcription factors such as E2Fa, as well as Ca2+/Calmodulin proteins in GABA biosynthesis in plants. GABA is known to improve stress tolerance by improving photosynthetic activity, C/N metabolism, stomatal conductance, and stress‐induced reactive oxygen species (ROS) detoxification. Here, we have reviewed recent studies that have explored the novel roles of GABA in plants with a focus on plant development and stress resilience.

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Antifungal mechanisms of γ-aminobutyric acid against the postharvest pathogen Alternaria alternata

Cited by 14 publications

References 37 publications

Non-proteinogenic amino acids mitigate oxidative stress and enhance the resistance of common bean plants against Sclerotinia sclerotiorum

Non-proteinogenic amino acids mitigate oxidative stress and enhance the resistance of common bean plants against Sclerotinia sclerotiorum

Nanofungicides with Selenium and Silicon Can Boost the Growth and Yield of Common Bean (Phaseolus vulgaris L.) and Control Alternaria Leaf Spot Disease

GABA in plants: developmental and stress resilience perspective

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