Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots

Nally, María Cristina; Pesce, Virginia Mercedes; Maturano, Yolanda Paola; Assaf, Leticia Anahí Rodríguez; Toro, María Eugenia; Figueroa, Lucía I. C. de; Vázquez, Fabio

doi:10.1016/j.ijfoodmicro.2015.03.024

Cited by 52 publications

(36 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among different microbial antagonists, yeasts have been of special interest due to their general lack of antibiotic production (Nally et al 2015;Pantelides et al 2015;Parafati et al 2015), rapid colonization of the fruit surface and wound sites (Castoria et al 2005), abiotic stress tolerance Wang et al 2010a), and compatibility with fungicides and other chemicals (Lima et al 2011;Lu et al 2014). Therefore, antagonistic yeasts are considered to have significant potential for reducing or replacing the use of chemical fungicides (Cao et al 2013;El Ghaouth et al 2003;Janisiewicz et al 2014;Lu et al 2014;Zhu et al 2015).…”

Section: Introductionmentioning

confidence: 99%

The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit

Yan

Wilson³

et al. 2015

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

Cold-adapted yeasts were isolated from soil samples collected in Tibet and evaluated as potential biocontrol agents against blue mold (Penicillium expansum) of pear fruit in cold storage. YC1, an isolate identified as Rhodotorula mucilaginosa, was found to exhibit the greatest biocontrol activity among the different isolates that were screened. A washed cell suspension of YC1 exhibited the best biocontrol activity among three different preparations that were used in the current study. A concentration of 10(8) cells/ml reduced the incidence of decay to 35 %, compared to the control where decay incidence was 100 %. A higher intracellular level of trehalose and a higher proportion of polyunsaturated acids present in YC1, was associated with increased the tolerance of this strain to low temperatures, relative to the other strains that were evaluated. The increased tolerance to low temperature allowed the YC1 strain of yeast to more effectively compete for nutrients and space in wounded pear fruit that had been inoculated with spores of P. expansum and placed in cold storage. The present study demonstrated the ability to select cold-adapted yeasts from cold climates and use them as biocontrol agents of postharvest diseases of fruit placed in cold storage.

show abstract

Section: Introductionmentioning

confidence: 99%

The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit

Yan

Wilson³

et al. 2015

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

show abstract

“…; Nally et al . ), bacteria–bacteria (Jaspers and Overmann ) and bacteria–filamentous fungi (Nesci et al . ) have been previously studied.…”

Section: Resultsmentioning

confidence: 99%

“…The major mode of action of these yeasts is the competition for nutrient and space (Nally et al . ). Few data have been published about the influence of yeast‐based biofungicides used in vineyards on grape fermentation (Calvo‐Garrido et al .…”

Section: Introductionmentioning

confidence: 97%

“…Among the various potential antagonists, yeasts have been studied as fungal biocontrol agents on grapes (Ponsone et al 2011(Ponsone et al , 2016Nally et al 2012Nally et al , 2013Calvo-Garrido et al 2013). The major mode of action of these yeasts is the competition Letters in Applied Microbiology 67, 89--96 © 2018 The Society for Applied Microbiology for nutrient and space (Nally et al 2015). Few data have been published about the influence of yeast-based biofungicides used in vineyards on grape fermentation (Calvo-Garrido et al 2013;Guzzon et al 2014).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of behaviour ofLachancea thermotoleransbiocontrol agents on grape fermentations

Nally

Ponsone

Pesce

et al. 2018

Lett Appl Microbiol

Self Cite

View full text Add to dashboard Cite

Generally it is not evaluated if the biofungicide yeasts sprayed on vegetables alter the quality of the fermented products. This work focused on the importance of assessing the possible effects of yeast-based fungicides used in vineyards on grape fermentation, especially on Saccharomyces cerevisiae growth. In this context, the competition between biofungicide yeasts and S. cerevisiae under winemaking conditions is investigated.

show abstract

“…For example, the production of exo-glucanase toxins in W. anomalus was reported by Parafati et al (2016) [156], but the production of certain volatile organic compounds (i.e., 2-phenylethanol) has also been suggested as determinant for the biocontrol activity against B. cinerea found in this species and in others such as Candida intermedia , Sporidiobolus pararoseus , Starmerella bacilaris and S. cerevisiae [157,158,159,160,161,162]. …”

Section: Applications Conclusion and Future Perspectives Of P Mmentioning

confidence: 93%

The Biology of Pichia membranifaciens Killer Toxins

Belda

Ruíz

Alonso

et al. 2017

Toxins

View full text Add to dashboard Cite

The killer phenomenon is defined as the ability of some yeast to secrete toxins that are lethal to other sensitive yeasts and filamentous fungi. Since the discovery of strains of Saccharomyces cerevisiae capable of secreting killer toxins, much information has been gained regarding killer toxins and this fact has substantially contributed knowledge on fundamental aspects of cell biology and yeast genetics. The killer phenomenon has been studied in Pichia membranifaciens for several years, during which two toxins have been described. PMKT and PMKT2 are proteins of low molecular mass that bind to primary receptors located in the cell wall structure of sensitive yeast cells, linear (1→6)-β-d-glucans and mannoproteins for PMKT and PMKT2, respectively. Cwp2p also acts as a secondary receptor for PMKT. Killing of sensitive cells by PMKT is characterized by ionic movements across plasma membrane and an acidification of the intracellular pH triggering an activation of the High Osmolarity Glycerol (HOG) pathway. On the contrary, our investigations showed a mechanism of killing in which cells are arrested at an early S-phase by high concentrations of PMKT2. However, we concluded that induced mortality at low PMKT2 doses and also PMKT is indeed of an apoptotic nature. Killer yeasts and their toxins have found potential applications in several fields: in food and beverage production, as biocontrol agents, in yeast bio-typing, and as novel antimycotic agents. Accordingly, several applications have been found for P. membranifaciens killer toxins, ranging from pre- and post-harvest biocontrol of plant pathogens to applications during wine fermentation and ageing (inhibition of Botrytis cinerea, Brettanomyces bruxellensis, etc.).

show abstract

Antifungal modes of action of Saccharomyces and other biocontrol yeasts against fungi isolated from sour and grey rots

Cited by 52 publications

References 72 publications

The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit

The ability of a cold-adapted Rhodotorula mucilaginosa strain from Tibet to control blue mold in pear fruit

Evaluation of behaviour ofLachancea thermotoleransbiocontrol agents on grape fermentations

The Biology of Pichia membranifaciens Killer Toxins

Contact Info

Product

Resources

About