Antifungal susceptibility testing of vulvovaginal &lt;em&gt;Candida&lt;/em&gt; species among women attending antenatal clinic in tertiary care hospitals of Peshawar

Khan, Maria; Ahmed, Jawad; Gul, Amina; Ikram, Aamer; Lalani, Farida Khurram

doi:10.2147/idr.s153116

Cited by 67 publications

(74 citation statements)

References 29 publications

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“…China, Brazil, Tunis, Kuwait, India, Greece and Turkey have reported that C.albicans remains the most commonly isolated yeast (60%-80%) in women diagnosed with VVC [21][22][23][24][25][26][27]. On the other hand, an increasing trend in the occurrence of NCAC (58%-60%) over time has also been observed in Pakistan and Burkina Faso [28,29]. The main identified NCAC in our study were C.glabrata (71%, N = 41) followed by C. krusei (29%, N = 17).…”

Section: Resultsmentioning

confidence: 98%

Prevalence and Antifungal Susceptibility of Candida albicans Causing Vaginal discharge Among Pregnant Women in Lebanon

Ghaddar

Anastasiadis

Halimeh

et al. 2019

Preprint

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Background: Vaginal candidiasis is frequently prevalent in pregnant women and is associated with sepsis and adverse neonatal outcomes. This study determined the presence of Candida species in symptomatic pregnant women and evaluated the antifungal susceptibility profile of the isolated strains. It also aimed to explore whether Candida species predicts gestational complications and adverse neonatal outcomes. Methods: A total of 258 pregnant women at 35 to 37 week of gestation participated in this study. Vaginal swabs from these patients were collected at various obstetrics and gynecology clinics in Lebanon for a period of 12 months. Candida isolates were identified at species level and antifungal susceptibility of Candida albicans to fluconazole (FCZ), amphotericin B (AMB), itraconazole (ICZ) and voriconazole (VCZ) was determined by the agar-based E-test method. Results: Among 258 women tested, 100 (39%) were positive for Candida species. C. albicans, C. glabrata and C. krusei were isolated from 42%, 41% and 17% of the women, respectively. C. albicans had significant positive associations with gestational diabetes while C. kreusi or C. glabrata had significant positive associations with gestational complications and vaginal discharge. The antifungal susceptibility tests of C. albicans isolates revealed 97.5%, 90%, 87.5% and 97.5% susceptibility to AMB, FCZ, ICZ and VCZ, respectively. Conclusion: The current study revealed high incidence of both C. albicans and non-C. albicans strains causing vulvovaginitis among pregnant women in Beirut, Lebanon. Whereas the susceptibility rates of C. albicans against AMB and VCZ were high, FCZ and ICZ proved comparatively less efficacious. The resistance profile of circulating C. albicans among pregnant women can predict the best outcome of appropriate prophylaxis or treatment of neonatal candidiasis. Vaginal candida colonization might lead to adverse neonatal outcome or gestational complications thus Candida screening as antennal follow up is advised.

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Section: Resultsmentioning

confidence: 98%

Prevalence and Antifungal Susceptibility of Candida albicans Causing Vaginal discharge Among Pregnant Women in Lebanon

Ghaddar

Anastasiadis

Halimeh

et al. 2019

Preprint

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“…According to various data (Bradford & Ravel, 2017;Bitew & Abebaw, 2018), the detection rate of C. albicans is at least 35-45%, which is confirmed by the results obtained by us. Accordingly, the total frequency of detection of nonalbicans Candida species is more than 50% (Liu et al, 2014;Khan et al, 2018). Separately, species such as C. glabrata and C. krusei are quite common.…”

Section: Discussionmentioning

confidence: 99%

“…It is necessary to perform correction of the conditions that lead to the development of genital candidiasis. Use of multivitamin complexes may be beneficial (Mendling et al, 2015;Pappas et al, 2016;Khan et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Vaginal candidiasis among women of reproductive age in Dnipro city (Ukraine): Species spectrum of Candida and their susceptibility to antimycotics

Starishko¹,

Voronkova²,

Ostanina³

et al. 2019

Regul. Mech. Biosyst.

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Candidiasis is one of the most common pathological processes, especially among women of reproductive age, especially pregnant. It can be expressed as Candida carriage and as an active form of infection. Although candidiasis is caused by opportunistic microorganisms, its effects can be quite significant, so it is necessary to treat it with the use of antimycotic drugs. In view of the spread among these microorganisms of resistance to antimicrobial drugs, the determining factor for the success of therapeutic measures, it is important to make a preliminary assessment of the susceptibility to antimycotics of strains isolated from persons with Candida carriage or active candidiasis. The aim of the research was to study the species range of fungi of the genus Candida isolated from the reproductive tract in women with dysbiosis and to study their susceptibility to antimycotics. Total of 227 samples of biological material from women aged 16 to 56 years have been investigated. Microscopic and cultural methods of detecting of fungi in the primary material were used for research. For the identification of species of Candida fungi, a polymerase chain reaction method in real-time was used. It was determined that the frequency of detection of Candida fungi in the composition of vaginal microbiome during inflammatory pathological processes was 76.7%. It was shown that the incidence of Candida carriage – 51.7% – slightly exceeded the frequency of detection of the active form of infection – 48.3%. The species spectrum of pathogens included: C. albicans (57.1%), C. krusei (12.6%), C. glabrata (19.5%) and Candida spp. (16.1%). In the case of active form of infection, C. albicans was prevalent – 64 (76.2%) cases. In the case of Candida carriage, other species were prevalent – 58 (64.4%) cases. The most effective drug against all isolates was amphotericin B – more than 87% of isolates were susceptible. The least effective drug was nystatin: less than 60% of isolates were susceptible. There is a rather high level of resistance to certain antimycotic drugs among the Candida species, which requires a prior preliminary study of the susceptibility of isolated strains to antimycotics for the purpose of choosing a rational and effective treatment scheme.

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“…Pregnant women in the third trimester were mostly affected by VVC unlike a similar study in Peshawar which reported most infections in the second trimester. [26] C infections. [31] In such cases, it's recommended that liposomal amphotericin B is used in addition to managing the source of the infection.…”

Section: Prevalence and Phenotypic Characterization Ofmentioning

confidence: 99%

Prevalence and Antifungal Susceptibility patterns of Candida isolated on CHROMagarTMCandida at a tertiary referral hospital, Eastern Uganda.

Watsemwa

Iramiot

Kalule

2019

Preprint

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Background Pregnant women are susceptible to vaginal candidiasis and maternal vulvovaginal candidiasis is a major risk factor for colonization and/or infection of the infant. The purpose of this study was to determine the prevalence and antifungal patterns of albicans and non-albicans Candida among pregnant women attending a tertiary referral hospital. Methods Vaginal discharge- cotton swabs were self-collected from pregnant women clinically proven to have vulvovaginal candidiasis at the antenatal clinic of a tertiary referral hospital between January and July 2018. Microscopy and culture on Sabouraud's Dextrose Agar with chloramphenicol was done on the vaginal discharge-cotton swabs. Confirmatory fungal identification was done using CHROM agarTM Candida. Antifungal susceptibility testing was carried out using the standardized Kirby Bauer dilution method. Results Candida were isolated from 50.81% (126/249) of the swabs and included C. albicans (80.16%, 101/126), C. glabrata (19.05% (24/126) and C. krusei (0.79%, 1/126). Of the women from whom Candida were isolated, 11.1% (14/126) were in the first trimester, 39.7% (50/126) were in the second, while 49.2% (62/126) were in the third. Of the Candida isolates, 80.16% (101/126) were C. albicans, 19.05% (24/126) were C. glabrata and 0.79% (1/126) were C. krusei. Overall, all the isolates were non-susceptible to Amphotericin B, while 60.3% (76/126), 50% (63/126), 62.7% (79/126), and 48.4% (61/126) were non-susceptible to itraconazole, fluconazole, nystatin, and clotrimazole respectively. All the non-albicans Candida were resistant to itraconazole, amphotericin B, and fluconazole. Conclusion Vulvovaginal candidiasis due to multidrug resistant C.glabrata among pregnant women will require that treatment regimes are adjusted to carter for the recurrent forms. The use of CHROMagarTMCandida media for identification of clinically relevant Candida should be adopted instead of conventional methods that are tedious and time consuming such that treatment is based on laboratory evidence.

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Antifungal susceptibility testing of vulvovaginal <em>Candida</em> species among women attending antenatal clinic in tertiary care hospitals of Peshawar

Cited by 67 publications

References 29 publications

Prevalence and Antifungal Susceptibility of Candida albicans Causing Vaginal discharge Among Pregnant Women in Lebanon

Prevalence and Antifungal Susceptibility of Candida albicans Causing Vaginal discharge Among Pregnant Women in Lebanon

Vaginal candidiasis among women of reproductive age in Dnipro city (Ukraine): Species spectrum of Candida and their susceptibility to antimycotics

Prevalence and Antifungal Susceptibility patterns of Candida isolated on CHROMagarTMCandida at a tertiary referral hospital, Eastern Uganda.

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