Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish

Asha, Arambam Devi; Nayak, Dhirendra Kumar; Shankar, K. M.; Mohan, C. V.

doi:10.1016/j.fsi.2003.08.001

Cited by 31 publications

(19 citation statements)

References 35 publications

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“…The results also showed that this approach has to be applied on the right age, because too young animals still developed a kind of "neonatal" tolerance (immune-suppression). Other attempts to protect antigens for degradation in the gut are the use of (Eudragit) coated bacteria [151], microbial biofilms [152,153] and recently bacterial ghosts [154]. A variety of microparticles have been tested and most studies report a better delivery in the endgut, elevated antibody production and/or higher protection using alginate microparticles [12,155,156], liposomes [157,158], poly D,L-lactide-co-glycolic acid (PLGA) microparticles [33e35, 159,160] and chitosan microparticles [161,162].…”

Section: Oral Vaccination Strategiesmentioning

confidence: 99%

Teleost intestinal immunology

Rombout¹,

Abelli²,

Picchietti³

et al. 2011

Fish & Shellfish Immunology

510

284

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Teleosts clearly have a more diffuse gut associated lymphoid system, which is morphological and functional clearly different from the mammalian GALT. All immune cells necessary for a local immune response are abundantly present in the gut mucosa of the species studied and local immune responses can be monitored after intestinal immunization. Fish do not produce IgA, but a special mucosal IgM isotype seems to be secreted and may (partly) be the recently described IgZ/IgT. Fish produce a pIgR in their mucosal tissues but it is smaller (2 ILD) than the 4-5 ILD pIgR of higher vertebrates. Whether teleost pIgR is transcytosed and cleaved off in the same way needs further investigation, especially because a secretory component (SC) is only reported in one species. Teleosts also have high numbers of IEL, most of them are CD3-ɛ+/CD8-α+ and have cytotoxic and/or regulatory function. Possibly many of these cells are TCRγδ cells and they may be involved in the oral tolerance induction observed in fish. Innate immune cells can be observed in the teleost gut from first feeding onwards, but B cells appear much later in mucosal compartments compared to systemic sites. Conspicuous is the very early presence of putative T cells or their precursors in the fish gut, which together with the rag-1 expression of intestinal lymphoid cells may be an indication for an extra-thymic development of certain T cells. Teleosts can develop enteritis in their antigen transporting second gut segment and epithelial cells, IEL and eosinophils/basophils seem to play a crucial role in this intestinal inflammation model. Teleost intestine can be exploited for oral vaccination strategies and probiotic immune stimulation. A variety of encapsulation methods, to protect vaccines against degradation in the foregut, are reported with promising results but in most cases they appear not to be cost effective yet. Microbiota in fish are clearly different from terrestrial animals. In the past decade a fast increasing number of papers is dedicated to the oral administration of a variety of probiotics that can have a strong health beneficial effect, but much more attention has to be paid to the immune mechanisms behind these effects. The recent development of gnotobiotic fish models may be very helpful to study the immune effects of microbiota and probiotics in teleosts.

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Section: Oral Vaccination Strategiesmentioning

confidence: 99%

Teleost intestinal immunology

Rombout¹,

Abelli²,

Picchietti³

et al. 2011

Fish & Shellfish Immunology

510

284

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“…BF cells of A. hydrophila showed repression of nine proteins and additional expression of three proteins of 72, 36 and 22 kDa (Asha et al 2004). Similar observations were also made in Pseudomonas putida where 15 proteins were up-regulated and 30 proteins were down-regulated following attachment to silicon surface (Sauer and Camper 2001).…”

Section: Resultsmentioning

confidence: 97%

“…Changes in the protein profile of BF cells in comparison with free cells (FC) of A. hydrophila used in oral vaccination of fish have been reported (Asha et al 2004). Cellsurface structures, cell wall composition, exopolysaccharide and capsular polysaccharides present in the bacteria vary with growth conditions.…”

Section: Introductionmentioning

confidence: 99%

Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp

et al. 2010

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Biofilm (BF) of Vibrio alginolyticus was developed on chitin flakes. BF formation was studied at various nutrient concentrations and incubation time. Highest colonyforming units of BF cells were obtained with 0.15% trypticase soya broth and at 3 days of incubation. The BF cells could be completely inactivated at 80°C in 10 min and with 10% formalin in 24 h. SDS-PAGE profile of BF cells revealed repression of four proteins and expression of three new proteins compared to free cells (FC). The preliminary immune response studies showed that BF cells were superior to FC in stimulating the non-specific immune response of Penaeus monodon.

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“…Immunization with polyvalent vaccines, heat-killed and live attenuated Aeromonas hydrophila has been reported [6]- [9]. Biofilm of Aeromonas hydrophila has been shown to offer protection against bacterial challenge [10]. However, studies on immunization with a specific antigen(s) have remained limited to laboratories only.…”

Section: Introductionmentioning

confidence: 99%

Heterologous Soluble Expression of Recombinant OmpR of <i>Aeromonas hydrophila</i> and Its Immunogenic Potential

Yadav¹,

Marbaniang²,

Sharma³

et al. 2015

ABB

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Aeromonas hydrophila, a gram negative bacterium is a major fish pathogen and causes major economic losses to aquaculture industry. Outer membrane proteins play a significant role in its survival during different environmental conditions and bacterial pathogenesis. The outer membrane protein R (OmpR) is a member of the two-component regulatory system of Aeromonas hydrophila which differentially regulates the expression of OmpF or OmpC depending on the osmolarity conditions. Role of OmpR has been demonstrated in its virulence in other infectious bacteria and it is found to be a potential drug target/vaccine candidate. However, the OmpR of A. hydrophila has not been characterized. In the present study, we report recombinant expression, purification of the OmpR of A. hydrophila strain Ah17 in salt inducible E. coli GJ1158 cells. Leaky expression of rOmpR was confirmed by Western blot analysis using anti-6 × His antibody. The histidine tagged recombinant OmpR (rOmpR) (~29 kDa) was purified using Ni-NTA affinity chromatography from the soluble fraction of induced E. coli cells. The rOmpR was found to be highly immunogenic with end point titres of greater than 1:80,000. The anti-rOmpR antisera were capable of agglutinating live A. hydrophila cells, thus showing vaccine potential of the rOmpR.

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Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish

Cited by 31 publications

References 35 publications

Teleost intestinal immunology

Teleost intestinal immunology

Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp

Heterologous Soluble Expression of Recombinant OmpR of <i>Aeromonas hydrophila</i> and Its Immunogenic Potential

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Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish

Cited by 31 publications

References 35 publications

Teleost intestinal immunology

Teleost intestinal immunology

Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp

Heterologous Soluble Expression of Recombinant OmpR of &lt;i&gt;Aeromonas hydrophila&lt;/i&gt; and Its Immunogenic Potential

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Heterologous Soluble Expression of Recombinant OmpR of <i>Aeromonas hydrophila</i> and Its Immunogenic Potential